Effect of Sulphur Supply on the Seed Globulin Composition of Lupinus angustifolius

Blagrove, R.J.; Gillespie, JM; Randall, P. J.

doi:10.1071/pp9760173

Cited by 72 publications

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“…Legumin was virtually undetectable under severe S deficiency. Similar changes were found in the more S-rich com-47 ponent of the storage proteins of lupin under S deficiency conditions (1).…”

supporting

confidence: 67%

Regulation of Legumin Levels in Developing Pea Seeds under Conditions of Sulfur Deficiency

Chandler¹,

Higgins²,

Randall³

et al. 1983

Plant Physiol.

120

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It was shown previously that when peas (Pisum sativum L.) are grown with suboptimal sulfur supply the level of legumin (the more S-rich of the two major seed storage proteins) in the mature seed is selectively reduced (Randall, Thomson, Schroeder, 1979 Aust J Plant Physiol 6: [11][12][13][14][15][16][17][18][19][20][21][22][23][24]. This paper reports a study of the cellular mechanisms involved in regulating legumin synthesis under these conditions. Pulse and pulse-chase labeling experiments were carried out with excised, immature cotyledons from normal and S-deficient plants. Legumin Legumin and vicilin are the two storage proteins accumulated in large quantities during the formation of pea (Pisum sativum L.) seeds. In the cultivar used in our experiments, legumin makes up approximately 15% of the total protein in the seed at maturity (25). Legumin has a higher content of cysteine and methionine than vicilin and could therefore be considered to be the more desirable protein from the point of view of animal nutrition. Its physicochemical properties are typical of one of the storage proteins found in a range of legume seeds including broad bean ( Vicia faba), lupin (Lupinus angustifolius), and soybean (Glycine max) (8).It was shown earlier (19) that when peas are grown with suboptimal sulfur (S) nutrition there is a marked, selective reduction in the level of accumulated legumin relative to the total protein level. Legumin was virtually undetectable under severe S deficiency. Similar changes were found in the more S-rich com-47 ponent of the storage proteins of lupin under S deficiency conditions (1).This paper describes experiments that were designed to study the cellular mechanisms responsible for the reduction in the level of legumin in pea seeds developing under conditions of S deficiency. The following three mechanisms were tested: (a) a normal rate of legumin synthesis but an increased rate of degradation; (b) a normal level of legumin mRNA but reduced translation; and (c) reduced levels of legumin mRNA. The results indicate that a reduced level of legumin mRNA is the main factor responsible for the reduced accumulation of legumin. MATERIALS AND METHODSPlant Material. Peas (Pisum sativum L.) line PI/G 086, selected from cv Greenfeast, were grown in artificially lit cabinets at 20°C with a 16-h photoperiod as described (16) and pods of a known age, specified as DAF 1, were used. Plants were grown in sand with nutrient solution containing combined nitrogen (19). Sulfur was supplied as MgSO4 at I mm in the control nutrient throughout growth and 0.05 mm in the S-deficient nutrient until first flower and omitted thereafter. The level of Mg2+ was kept constant by varying MgCl2. Under these conditions, S-deficient plants showed characteristic symptoms and were smaller with fewer pods. Mean seed weight at maturity was little affected in pods from lower nodes but considerably reduced in later formed pods. For uniformity, only pods from the third to the sixth flowering node were used. Mature seeds from t...

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supporting

confidence: 67%

Regulation of Legumin Levels in Developing Pea Seeds under Conditions of Sulfur Deficiency

Chandler¹,

Higgins²,

Randall³

et al. 1983

Plant Physiol.

120

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“…In conditions of sulfur limitation, lupin seeds accumulate increased amounts of sulfur-poor storage proteins, and decreased amounts of relatively sulfur-rich proteins like conglutins-␣ and -␥ (Blagrove et al, 1976). Similarly, we have observed that mRNA coding for the very Cys-rich conglutin-␦ is diminished in control lupin seeds grown with low and medium sulfur compared with those grown in high sulfur medium (data not shown).…”

Section: Effects Of Manipulating Sulfur Supplysupporting

confidence: 52%

“…There are many reports of sulfur limitation evoking specific changes in protein profiles of seeds (Chandler et al, 1984;Gayler and Sykes, 1985), including narrow leaf lupin (Blagrove et al, 1976). In general, the accumulation of sulfur-rich proteins is down-regulated, whereas sulfur-poor proteins become overrepresented.…”

Section: Limits To the Accumulation Of Sulfur Amino Acids In Tg Lupinmentioning

confidence: 99%

“…4). These proteins probably correspond to endogenous sulfur-rich pro- Limits to Sulfur Accumulation in Transgenic Lupin Seeds teins whose abundance is controlled by sulfur availability (Blagrove et al, 1976).…”

Section: Protein Composition Of Mature Control and Tg Lupin Seedsmentioning

confidence: 99%

“…Conglutins gamma and delta are sulfur-rich storage proteins of lupin seeds (Blagrove et al, 1976;Lilley and Inglis, 1986). Northern-blot analysis revealed that the levels of mRNA coding for conglutin-␦ were strongly diminished in Tg:Med cotyledons expressing SSA, compared with control:Med cotyledons (Fig.…”

Section: Protein Composition Of Mature Control and Tg Lupin Seedsmentioning

confidence: 99%

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Limits to Sulfur Accumulation in Transgenic Lupin Seeds Expressing a Foreign Sulfur-Rich Protein

Tabe

Droux

2002

Plant Physiology

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The low sulfur amino acid content of legume seeds restricts their nutritive value for animals. We have investigated the limitations to the accumulation of sulfur amino acids in the storage proteins of narrow leaf lupin (Lupinus angustifolius) seeds. Variation in sulfur supply to lupin plants affected the sulfur amino acid accumulation in the mature seed. However, when sulfur was in abundant supply, it accumulated to a large extent in oxidized form, rather than reduced form, in the seeds. At all but severely limiting sulfur supply, addition of a transgenic (Tg) sink for organic sulfur resulted in an increase in seed sulfur amino acid content. We hypothesize that demand, or sink strength for organic sulfur, which is itself responsive to environmental sulfur supply, was the first limit to the methionine (Met) and cysteine (Cys) content of wild-type lupin seed protein under most growing conditions. In Tg, soil-grown seeds expressing a foreign Met-and Cys-rich protein, decreased pools of free Met, free Cys, and glutathione indicated that the rate of synthesis of sulfur amino acids in the cotyledon had become limiting. Homeostatic mechanisms similar to those mediating the responses of plants to environmental sulfur stress resulted in an adjustment of endogenous protein composition in Tg seeds, even when grown at adequate sulfur supply. Uptake of sulfur by lupin cotyledons, as indicated by total seed sulfur at maturity, responded positively to increased sulfur supply, but not to increased demand in the Tg seeds.

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Einfluß der Schwefeldüngung auf Ertrag und Qualitätsmerkmale bei einem Gefäßversuch mit Winterweizen

Sotiriou

Kick

1983

J Plant Nutrition & Soil

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Auf einem praktisch schwefelfreien Substrat in Gefäßversuchen wurden durch S‐Düngung Korn‐und Stroherträge sowie auch das Tausendkorngewicht von Winterweizen (Sorte: Breustedts Werla) signifikant erhöht und das N/S‐Verhältnis sowohl im Korn als auch im Stroh erniedrigt. Im Gegensatz dazu konnte bei den Versuchen ohne S‐Düngung der S‐Bedarf des angebauten Weizens aus der Atmosphäre als S‐Quelle nicht gedeckt werden. Die in dieser Arbeit vorgenommene Proteinfraktionierung in „freie Aminosäuren”︁, Albumin, Gliadin und Glutein ließ eine Veränderung der Proteinzusammensetzung durch S‐Düngung nicht erkennen. Dagegen wurde die Kleberqualität des Weizens, gemessen am Sedimentationstest (Mikro‐Zeleny‐Test), durch Schwefel merklich verbessert.

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Effect of Sulphur Supply on the Seed Globulin Composition of Lupinus angustifolius

Cited by 72 publications

References 0 publications

Regulation of Legumin Levels in Developing Pea Seeds under Conditions of Sulfur Deficiency

Regulation of Legumin Levels in Developing Pea Seeds under Conditions of Sulfur Deficiency

Limits to Sulfur Accumulation in Transgenic Lupin Seeds Expressing a Foreign Sulfur-Rich Protein

Einfluß der Schwefeldüngung auf Ertrag und Qualitätsmerkmale bei einem Gefäßversuch mit Winterweizen

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