Effect of Sulfur Supply on the Seed Globulin Composition of Various Species of Lupin

Gillespie, JM; Blagrove, R.J.; Randall, P. J.

doi:10.1071/pp9780641

Cited by 22 publications

(17 citation statements)

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“…1). In addition, the changes in the proportions of the two major types of storage proteins in sulfur-deficient soybeans closely followed the trends previously observed for equivalent legumin-like and vicilin-like proteins in other sulfur-deficient legumes (3,7) and were by contrast the reciprocal ofchanges observed in soybean cultured in the presence of excess methionine (4). In all species examined so far, the legumin-like proteins which contain the higher levels of sulfur amino acids were suppressed in plants grown on low sulfur, while the vicilin-like proteins with their lower levels of sulfur amino acids were enhanced.…”

Section: Discussionsupporting

confidence: 59%

Effects of Nutritional Stress on the Storage Proteins of Soybeans

Gayler

Sykes²

1985

Plant Physiol.

128

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The effects of sulfur deficiency on the complement of proteins laid down in developing seeds of soybean (Glycine max L. Meff) have been examined. Sulfur deficiency caused a 40% decrease in the level of glycinins and a contrasting elevation in the level of 6-conglycinins. The subunit composition of these proteins was also affected. There was in particular a 3-fold increase in the j-subunit of 6-conglycinins in the sulfur-deficient seeds, and this accumulated largely as the B,-isomer of ,B-conglycinins, a protein which while virtually devoid of methionine and cysteine retains the physical properties of a normal 7S storage protein.These data demonstrate that a high degree of selectivity can be exerted by environmental stress over the accumulation of proteins in developing seeds.The amino acid compositions ofglycinins and 13-conglycinins, the major types of storage proteins of soybean (Glycine max L., Merr), vary sufficiently to affect their nutritional value in animal diets. Glycinins, in common with other legumin-like proteins from peas (Pisum sativum), lupin (Lupinus angustifolius), and broad bean (Vicia faba), contain more of the limiting amino acids cysteine and methionine, than the fl-conglycinins and other vicilin-like proteins. The composition of the glycinins and f3-conglycinins present in different soybeans is, however, not constant.Both glycinins and ,.-conglycinins are families of proteins assembled from a number of different subunits and these subunits also differ in their contents of the sulfur containing amino acids. The range is from 0.6 to 3% (w/w) methionine plus cysteine between the extremes ofglycinin subunits (12) and from virtually zero to 0.48% (w/w) for the subunits of the f3-conglycinins (14). Such differences between subunits provide the basis for the production of storage protein complements of substantially different nutritional values, and this paper is part of a study on factors which can affect such production.In other legume species, alteration in the growth conditions of plants from a normal nutrient supply to suboptimal conditions has been shown to alter the proportions of the major storage proteins present. In both lupin (3) and peas (7), sulfur deficiency specifically depressed the synthesis of particular sulfur-rich proteins. In peas, sulfur deficiency operated specifically by repression of the levels of legumin mRNA (1).In deficiency causes the production of greatly enhanced levels of the B0-isomer2 of,-conglycinin, a protein which, while virtually devoid ofmethionine and cysteine, retains the physical properties of a normal 7S storage protein (13, 16). We have shown in addition that the proteins in soybean are responsive to even the mild sulfur deficiencies likely to occur under field conditions, and predict that the already low cysteine and methionine contents of the soybean will be further depressed under these conditions to the detriment of its nutritional value. Plants subjected to specific sulfur deficiency received an equivalent medium containing magnesium chlorid...

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Section: Discussionsupporting

confidence: 59%

Effects of Nutritional Stress on the Storage Proteins of Soybeans

Gayler

Sykes²

1985

Plant Physiol.

128

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“…Globulins, being the major seed storage proteins in legumes, had the highest relative content in mature soybean seeds and are synthesized during seed development on polysomes, transferred via lumen, sequestered and ultimately deposited in protein bodies (Kaviani and Kharabian, 2008). Increase in albumin and globulin content in chickpea seeds by various S sources including single super phosphate, gypsum and elemental S at all developmental stages (Ghalotra et al, 2007), by N and S in chickpea (Singh and Matta, 2005) and mungbean (Kumar et al, 2013) have been reported whereas seed globulin decreased in lupin seeds due to S deficiency (Gillespie et al, 1978). In the present studies, the increase in globulin and albumin contents in soybean seeds with N and S supply alone or in combination suggested that supply of various minerals can improve the storage protein fractions in soybean.…”

Section: Resultsmentioning

confidence: 99%

Effect of nitrogen and sulphur nutrition on yield parameters and protein composition in soybean [Glycine max (L.) Merrill].

Sharma

2014

JANS

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) and combined application of nitrogen (N) and sulphur (S) were investigated. Application of S alone or combined with N significantly increased plant height, number of seeds per pod and total seed yield. Sulphur-containing amino acids viz., methionine and cysteine increased significantly (P≤0.05) by all the treatments in comparison to control and maximum increase of 1.3 fold was observed by urea application. The contents of different protein fractions viz., albumin, globulin and glycinin (a subfraction of globulin) increased under the effect of N and S applied alone or in combination from 30 DAP to maturity with maximum accumulation at maturity as compared to control (without N or S application). Gypsum @ 20 kg S ha -1 resulted in higher accumulation of glycinin (11S) relative to β-conglycinin (7S) fraction of globulin protein in soybean seed thereby increasing 11S:7S ratio indicating improvement in soybean seed quality. The current study showed that improvement of soybean nutritional quality can be achieved by application of gypsum @ 20 kg S ha -1 along with recommended N doses under agroclimatic conditions of Punjab.

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“…Seeds from sulfur-stressed lupin plants accumulated the same amount of protein as sulfur-sufficient controls (4,10), but the sulfur-containing a-and y-subunits decreased in stressed plants, and the amount of the nonsulfur-containing lupin #-subunit apparently increased to compensate for the decline in aand 'y-subunits (4 (Table IV). Amino acid analysis showed that the a'-subunit components which accumulated in the presence ofmethionine had greater methionine contents than those which accumulated in controls (Table VIII).…”

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confidence: 99%

“…The ratio of sulfur to nitrogen decreases in proteins of sulfurstressed plants (1,4,(8)(9)(10)22 (29), composed a larger proportion of the 7S fraction of cotyledons on basal medium (>40%) ( Table IV) than seeds developed in vivo (25% to 30%) (Table III). Seeds from sulfur-stressed lupin plants accumulated the same amount of protein as sulfur-sufficient controls (4,10), but the sulfur-containing a-and y-subunits decreased in stressed plants, and the amount of the nonsulfur-containing lupin #-subunit apparently increased to compensate for the decline in aand 'y-subunits (4 (Table IV).…”

mentioning

confidence: 99%

“…Attempts have been made to increase protein sulfur in the vegetative tissues and seeds of a number of intact plants by applying high concentrations of sulfur fertilizers to the soil; however, excess sulfur does not increase the sulfur to nitrogen ratio of total protein (1,4,(8)(9)(10)22). There are no detectable changes in amino acid composition oftotal protein or in subunit content of the storage proteins of lupin seeds (4).…”

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confidence: 99%

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Effects of Exogenous Methionine on Storage Protein Composition of Soybean Cotyledons Cultured In Vitro

Holowach

Thompson²,

Madison³

1984

Plant Physiol.

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ABSTRAC1Supplemental methionine in a complete culture medium increased the methionine content of the protein fraction of cultured soybean (Glycine max L. Merrill) cotyledons (Thompson, Madison, Muenster 1981 Phytochemistry 20: 941-945). To explain the observed increase in protein methionine, we have measured the amounts and subunit compositions of 7S and 11S storage proteins and determined the amino acid compositions of the three major protein fractions (2-5S, 7S, 11S) of seeds developed on plants and of cultured cotyledons grown in the presence or absence of supplemental L-methionine. Development of cultured cotyledons was representative of development of seeds on plants. The ratios of 11S to 7S proteins, the subunit contents, and amino acid compositions of their storage protein fractions were similar, but not identical. Supplemental methionine increased the mole percent methionine in each of the three protein fractions of cultured cotyledons and changed the amounts of several other amino acids. Supplemental methionine inhibited expression of the 7S a-subunit gene. Concomitant with the absence of the a-subunit, which contains no methionine, was an increase in the ratio of 11S to 7S proteins, and an increase in the methionine content of the subunits composing these fractions. Inhibition of 6-subunit gene expression by methionine in cultured cotyledons provides a reproducible, easily controlled system for the study of eucaryotic gene expression.Soybean seed proteins lack sufficient quantities ofsulfur amino acids to satisfactorily meet the dietary requirements of some monogastric animals. In this respect, soybeans are typical legume seeds. Soybeans, however, accumulate about twice as much protein in their seeds as other legumes, and up to four times as much protein as grain seeds. The nutritional quality of soybean and other legume seeds could be improved by raising the sulfurcontaining amino acid content of their proteins (37). Since the 7S and Il S storage protein fractions constitute about 70% of total seed protein, they have been the focus of efforts to raise protein methionine content. The soybean 7S fraction consists of three major subunits, the a'-, a-, and a-subunits. (29,31) In vitro experiments were designed so that sets of 5 to 46 cotyledon pairs were subjected to control and experimental treatments. Individual seeds were divided so that one-half was placed in complete medium lacking methionine, and the other half in complete medium containing 8.4 mm L-methionine.Rates of growth and protein accumulation were variable in seeds grown on plants. Seeds matured more slowly in the winter than in the summer. Maturity was therefore measured by increments of seed fresh weight, which was found to be a more consistent measure of the time course of development than days after flowering (13,18

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Effect of Sulfur Supply on the Seed Globulin Composition of Various Species of Lupin

Cited by 22 publications

References 10 publications

Effects of Nutritional Stress on the Storage Proteins of Soybeans

Effects of Nutritional Stress on the Storage Proteins of Soybeans

Effect of nitrogen and sulphur nutrition on yield parameters and protein composition in soybean [Glycine max (L.) Merrill].

Effects of Exogenous Methionine on Storage Protein Composition of Soybean Cotyledons Cultured In Vitro

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