Effect of sulforaphane on apoptosis, reactive oxygen species and lipids peroxidation of human sperm during cryopreservation

Valipour, Jamal; Nashtaei, Maryam Shabani; Khosravizadeh, Zahra; Mahdavinezhad, Forough; Nekoonam, Saeid; Esfandyari, Sahar; Amidi, Fardin

doi:10.1016/j.cryobiol.2020.11.012

Cited by 21 publications

(19 citation statements)

References 55 publications

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“…Also, it increased TAC level. These results are in agreement with other studies (Feng et al, 2020; Shaygannia et al, 2020; Valipour et al, 2020).…”

Section: Discussionsupporting

confidence: 94%

“…The results showed that L-proline may protect chromatin integrity in cryopreserved spermatozoa. In line with these results, Santonastaso These results are in agreement with other studies (Feng et al, 2020;Shaygannia et al, 2020;Valipour et al, 2020).…”

Section: Discussionsupporting

confidence: 93%

“…However, there are positive correlation between over production of ROS and chromatin failure (Novotny et al, 2013). The oxidative stress is one of the primary reasons of chromatin integrity and all sperm parameters failures during cryopreservation (Valipour et al, 2020). Also, it seems that osmotic stress may play important role in overproduction of ROS (Ball, 2008).…”

Section: Discussionmentioning

confidence: 99%

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L‐proline as a novel additive to cryopreservation media improved post‐thaw quality of human spermatozoon via reducing oxidative stress

et al. 2021

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Sperm cryopreservation as a routine technique in assisted reproductive technique (ART) laboratories has detrimental effects on spermatozoa. Various methods have been introduced to improve it. The aim of this research was to evaluate the effects of L‐proline supplementation in cryopreservation medium on normozoospermic semen samples. A total of 30 semen samples were collected from normozoospermic men. Cryopreservation media were supplemented with different concentrations of L‐proline (0, 1, 2 and 4 mmol/L). The semen samples were cryopreserved. After thawing, sperm parameters and chromatin integrity (aniline blue (AB), toluidine blue (TB), sperm chromatin dispersion test (SCD) and chromomycin A3 (CMA3)), reactive oxygen species (ROS), and total antioxidant capacity (TAC) and malondialdehyde (MDA) levels were evaluated. A total of 4 mmol/L L‐proline significantly improved progressive motility and viability (p < 0.05). MDA and ROS levels significantly diminished in samples were cryopreserved by 4 mmol/L L‐proline supplemented cryopreservation media (p < 0.001). Also, it significantly increased TAC level. Also, chromatin damages (AB, TB and CMA3) significantly improved in samples were cryopreserved by 4 mmol/L L‐proline supplemented cryopreservation media (p < 0.05). The results support that the usage of L‐proline supplemented cryopreservation media to improve sperm quality after cryopreservation.

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“…Also, it increased TAC level. These results are in agreement with other studies (Feng et al, 2020; Shaygannia et al, 2020; Valipour et al, 2020).…”

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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L‐proline as a novel additive to cryopreservation media improved post‐thaw quality of human spermatozoon via reducing oxidative stress

et al. 2021

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“…ROS is one of the regulatory factors in chronic inflammation that can regulate other relevant factors in this process, such as activator protein 1 (AP‐1), NF‐κB, β‐catenin/Wnt (wingless related integration site), chemokines, inflammatory cytokines, peroxisome proliferator‐activated receptor‐gamma (PPAR‐γ), and growth factors. 63 , 64 , 65 , 66 In the presence of elevated ROS in the microenvironment, the secretion of VEGF and HIF1 would stimulate and activate cellular signaling related to migration and proliferation. 67 , 68 Therefore, targeting of ROS becomes another factor to consider as a therapeutic approach to modulate proliferative signaling and angiogenesis.…”

Section: The Molecular Mechanisms Behind the Microenvironment In Tumo...mentioning

confidence: 99%

The role of the tumor microenvironment in colorectal cancer and the potential therapeutic approaches

Zafari

Khosravi

Rezaee

et al. 2022

Clinical Laboratory Analysis

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Background: Colorectal cancer (CRC) with a high prevalence is recognized as the fourth most common cause of cancer-related death globally. Over the past decade, there has been growing interest in the network of tumor cells, stromal cells, immune cells, blood vessel cells, and fibroblasts that comprise the tumor microenvironment (TME) to identify new therapeutic interventions.Methods: Databases, such as Google Scholar, PubMed, and Scopus, were searched to provide an overview of the recent research progress related to targeting the TME as a novel therapeutic approach.Results: Tumor microenvironment as a result of the cross talk between these cells may result in either advantages or disadvantages in tumor development and metastasis, affecting the signals and responses from the surrounding cells. Whilst chemotherapy has led to an improvement in CRC patients' survival, the metastatic aspect of the disease remains difficult to avoid. Conclusions:The present review emphasizes the structure and function of the TME, alterations in the TME, its role in the incidence and progression of CRC, the effects on tumor development and metastasis, and also the potential of its alterations as therapeutic targets. It should be noted that providing novel studies in this field of research might help us to achieve practical therapeutic strategies based on their interaction.

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“…A complex change including ice crystal formation, osmotic changes, and physical and chemical stress during the cryopreservation process contribute to the damage to the cell membrane, internal structures, or gene expression, especially using the rapid or slow freezing techniques ( Hezavehei et al, 2018 ). Although vitrification reduces these effects and improves the survival rate, the cryopreservation of sperm, no matter which freezing technology, could still lead to significant changes in motility, DNA integrity, reactive oxygen species (ROS) levels, fertilizing ability, and gene expression profiles induced by these physical, chemical, and biological factors ( Hezavehei et al, 2018 ; Valipour et al, 2021 ). Therefore, further investigation of this subject will be beneficial for the development of sperm cryopreservation technologies and will assist people with fertility preservation, whether for medical or social needs.…”

Section: Introductionmentioning

confidence: 99%

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

Zhang

et al. 2021

Front. Cell Dev. Biol.

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Although sperm preservation is a common means of personal fertility preservation, its effects on embryonic development potential need further investigation. The purpose of this study was to identify key microRNA (miRNA) in cryopreserved sperm and determine the changes of these miRNAs and their target genes during embryonic development using cryopreserved sperm. Moreover, the embryonic development potential of cryopreserved sperm was estimated in assisted reproductive technology (ART), where key miRNAs and target genes were validated in sperm and subsequent embryos. Clinical data of embryonic development from cryopreserved sperm indicated a significant decrease in fertilization rate in both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cases, as well as a reduction in blastocyst formation rate in ICSI cases. Meanwhile there was a significant increase in blocked embryo ratio of Day1, Day2, and Day3.5 embryos when frozen-thawed mouse sperm was used, compared with fresh mouse sperm, suggesting a potential negative effect of sperm cryopreservation on embryonic development. From frozen-thawed and fresh sperm in humans and mice, respectively, 21 and 95 differentially expressed miRNAs (DEmiRs) were detected. miR-148b-3p were downregulated in both human and mouse frozen-thawed sperm and were also decreased in embryos after fertilization using cryopreserved sperm. Target genes of miR-148b-3p, Pten, was identified in mouse embryos using quantitative real-time PCR (qRT-PCR) and Western blot (WB). In addition, common characters of cryopreservation of mouse oocytes compared with sperm were also detected; downregulation of miR-148b-3p was also confirmed in cryopreserved oocytes. In summary, our study suggested that cryopreservation of sperm could change the expression of miRNAs, especially the miR-148b-3p across humans and mice, and may further affect fertilization and embryo development by increasing the expression of Pten. Moreover, downregulation of miR-148b-3p induced by cryopreservation was conserved in mouse gametes.

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Effect of sulforaphane on apoptosis, reactive oxygen species and lipids peroxidation of human sperm during cryopreservation

Cited by 21 publications

References 55 publications

L‐proline as a novel additive to cryopreservation media improved post‐thaw quality of human spermatozoon via reducing oxidative stress

L‐proline as a novel additive to cryopreservation media improved post‐thaw quality of human spermatozoon via reducing oxidative stress

The role of the tumor microenvironment in colorectal cancer and the potential therapeutic approaches

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

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