Effect of Sulfation on the Gastrointestinal Actions of Caerulein

Johnson, Leonard R.; Stening, G. Frank; Grossman, Morton I.

doi:10.1016/s0016-5085(70)80010-5

Cited by 56 publications

(13 citation statements)

References 18 publications

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“…Previous studies in dogs have indicated that caerulein was a strong peptic stimulant both in the Heidenhain pouch preparation (Bertaccini, Endean, Erspamer & Impicciatore, 1968) and in gastric fistula animals . Caerulein was twice as potent ) and later five times as potent (Johnson, Stening & Grossman, 1970) as desulphated caerulein in stimulating peptic secretion, although Way (1971) concluded that desulphated caerulein was the stronger peptic stimulant.…”

Section: Discussionmentioning

confidence: 99%

The influence of secretin on the secretion of pepsin in response to acid stimulants in the anaesthetized cat.

Braganza¹,

Gibbs²,

Howat³

1975

The Journal of Physiology

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Peptic secretion was studied in fasting anaesthetized cats in which the pylorus and common bile duct had been occluded to prevent the release of duodenal hormones which might stimulate or inhibit gastric secretion. Dilute acid was instilled into the stomach at intervals to aid recovery of gastric secretion and to preserve peptic activity. 2. Caerulein, histamine and N‐methyl histamine did not increase the output of pepsin when given on their own. Desulphated caerulein was a weak peptic stimulant. 3. Two C.H.R. u./kg per hour secretin initiated pancreatic secretion, the volume of which increased progressively as the dose was increased by stages to 32 C.H.R. u./kg per hour. 4. Four C.H.R. u./kg per hour secretin did not increase the output of pepsin. Peptic secretion was stimulated by 8 C.H.R. u./kg per hour. A maximal output of approximately 2000 u. pepsin/15 min was obtained when 16 C.H.R. u./kg per hour was infused. 5. When each acid stimulant was infused along with 4 C.H.R. u./kg per hour secretin the output of pepsin increased significantly. The peak output, which usually occurred between 15 and 30 min after stimulation, did not exceed 1000 u. pepsin/15 min. 6. The proposed explanation for the potentiation of the peptic response when an acid stimulant is infused along with a dose of secretin, in itself below the threshold of peptic stimulation, is that each acid stimulant increases gastric mucosal blood flow, approximately doubling the effective concentration of secretin delivered to the peptic cell.

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Section: Discussionmentioning

confidence: 99%

The influence of secretin on the secretion of pepsin in response to acid stimulants in the anaesthetized cat.

Braganza¹,

Gibbs²,

Howat³

1975

The Journal of Physiology

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“…Our experiments prove that they are also applicable to studies of gastric secretion in acute experiments in anaesthetized cats. Johnson, Stening & Grossman (1970) found caerulein, with an E.D.50 of 2*7 ng/kg min, 6*5 times more potent than desulphated caerulein in stimulating acid in gastric fistula dogs. In similar experiments in cats Way (1971) found caerulein 7 times more potent.…”

Section: Resultsmentioning

confidence: 86%

“…Similarly in fistula cats Code, Maslinski, Mossini & Navert (1971) found N methyl histamine 14-1-6 times more potent than histamine, an estimate which agrees with our conclusion in the presence of secretin, P816 (Table 3), but differs from the value of 5-63 when the stimulants were given singly. These discrepancies raise the possibility that in the work of Johnson et at. (1970), Dinbar & Grossman (1972) and Code et al (1971), the gastric fistulae may not have completely prevented the escape of acid into the duodenum leading to the release of endogenous secretin.…”

Section: Resultsmentioning

confidence: 94%

The interaction of secretin and stimulants of gastric acid secretion in anaesthetized cats.

Braganza¹,

Gibbs²,

Howat³

1976

The Journal of Physiology

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“…Like CCK, cerulein stimulates pancreatic and gastric secretions by binding to CCK receptors on the acinar cells. The natural sulfated version of cerulein, compared to the nonsulfated version, is far more effective in stimulating dose‐dependent pancreatic secretions than CCK …”

mentioning

confidence: 99%

“…The natural sulfated version of cerulein, compared to the nonsulfated version, is far more effective in stimulating dosedependent pancreatic secretions than CCK. 14 Administration of cerulein at a supramaximal dose (level above that which produces maximal secretion) results in less pancreatic secretion leaving the pancreas and an accumulation of digestive enzymes within the acinar cells. Zymogen granules "line up" at the apical end of the acinar cell, but secretory capacity is saturated.…”

mentioning

confidence: 99%

Quantitative Ultrasound and the Pancreas: Demonstration of Early Detection Capability

Miller

Han

Erdman

et al. 2018

J of Ultrasound Medicine

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Objectives To show that quantitative ultrasound biomarkers attenuation (AC) and backscatter (BSC) coefficients are effective tools to detect early changes in acute pancreatitis, using a cerulein‐induced pancreatitis rat model. Methods Sprague‐Dawley rats (n = 68) were divided into 8 groups: uninjected cage controls, saline‐injected controls, and cerulein‐injected rats euthanized at 2, 4, 15, 24, 48, and 60 hours after injection. Pancreatic AC and BSC (25–55 MHz) were estimated in vivo (Vevo 2100, VisualSonics, Toronto, CA) and ex vivo (40‐MHz transducer). The pancreas of each rat was evaluated histopathologically. Results Changes in both in vivo and ex vivo AC and BSC relative to controls reflected temporal histomorphologic changes. Overall, there were decreased AC and BSC at early time points and then rebound toward control values over time. Maximal in vivo AC and BSC decreases occurred at 2 hours after cerulein injection. Attenuation coefficient changes corresponded well with early pancreatic edema and acinar cell vacuolation, with rebound as edema decreased, autophagy/cellular death occurred, and histiocytic infiltrates and fibrosis manifested. Backscatter coefficient decreased early but rebounded as autophagy and apoptosis increased, only to fall as acinar atrophy peaked, and fibrosis and histiocytic infiltration increased. Conclusions Cerulein‐induced pancreatitis is an excellent model for studying ultrasonic AC and BSC biomarkers during the early stages of acute pancreatitits, reflecting microscopic structural changes. Edema followed by cell shrinkage and apoptosis, then histiocytic infiltration and fibrosis, has certain similarities with the morphologies of some forms of pancreatic carcinoma. This suggests that quantitative ultrasound may be very useful for early detection of disease onset or response to therapy for not only acute pancreatitis but also pancreatic cancer.

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Effect of Sulfation on the Gastrointestinal Actions of Caerulein

Cited by 56 publications

References 18 publications

The influence of secretin on the secretion of pepsin in response to acid stimulants in the anaesthetized cat.

The influence of secretin on the secretion of pepsin in response to acid stimulants in the anaesthetized cat.

The interaction of secretin and stimulants of gastric acid secretion in anaesthetized cats.

Quantitative Ultrasound and the Pancreas: Demonstration of Early Detection Capability

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