Effect of sugars on auxin-mediated LeEXPA2 gene expression

Arru, Laura; Rognoni, Sara; Poggi, Alessandra; Loreti, Elena

doi:10.1007/s10725-007-9252-5

Cited by 7 publications

(8 citation statements)

References 76 publications

(89 reference statements)

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“…Considering that we do not know how ACh could be taken up from the medium by the plant tissue, a concentration of 50 μM ACh was chosen for further experiments. From our previous work [ 42 ] we already know that if the segment elongation has to be monitored, the presence of sucrose is an essential requirement to sustain the auxin-induced LeEXPA2 transcript in a 16 h-long experiment. We are also aware that sucrose is more than an energy supply in the auxin induced expansin transcription [ 41 , 42 ], and we decided to take these observations into account in all the experimental conditions.…”

Section: Resultsmentioning

confidence: 99%

“…They allow turgor-driven cell expansion by disrupting hydrogen bonds of the wall polysaccharides, and they are involved in a variety of physiological responses such as root growth [ 58 ], internode elongation [ 59 ], leaf development [ 60 ], fruit development [ 61 ], floral development [ 62 ], fruit maturation [ 63 , 64 ], and seed germination [ 65 ]. They are expressed in the apical meristem [ 66 ] and in active elongating tomato hypocotyl tissues [ 41 , 42 , 67 ]; they also play important roles during abiotic stress response [ 68 – 70 ]. Expansin activity is shown to be regulated by many factors.…”

Section: Discussionmentioning

confidence: 99%

“…LeEXPA2 belongs to the expansins multigene family, and it is up regulated by auxin in the young growing regions of tomato hypocotyl [ 40 , 41 ]. We previously investigated the role of auxin and sugars on LeEXPA2 mRNA expression in elongating tomato hypocotyl segments [ 42 ]. Sugar cross-talk with hormonal signalling is well documented [ 43 – 45 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

New Insights on Plant Cell Elongation: A Role for Acetylcholine

Sansebastiano

Fornaciari

Barozzi

et al. 2014

IJMS

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We investigated the effect of auxin and acetylcholine on the expression of the tomato expansin gene LeEXPA2, a specific expansin gene expressed in elongating tomato hypocotyl segments. Since auxin interferes with clathrin-mediated endocytosis, in order to regulate cellular and developmental responses we produced protoplasts from tomato elongating hypocotyls and followed the endocytotic marker, FM4-64, internalization in response to treatments. Tomato protoplasts were observed during auxin and acetylcholine treatments after transient expression of chimerical markers of volume-control related compartments such as vacuoles. Here we describe the contribution of auxin and acetylcholine to LeEXPA2 expression regulation and we support the hypothesis that a possible subcellular target of acetylcholine signal is the vesicular transport, shedding some light on the characterization of this small molecule as local mediator in the plant physiological response.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

New Insights on Plant Cell Elongation: A Role for Acetylcholine

Sansebastiano

Fornaciari

Barozzi

et al. 2014

IJMS

Self Cite

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“…They are involved in many other processes and act also as metabolite signalling molecules that activate specific or hormone-crosstalk transduction pathways, resulting in important modifications of gene expression (Smeekens, 2000;Rolland et al, 2002;Gibson, 2005;Koch, 1996). Arru et al (2008) showed that sucrose at concentrations of range of 15 to 120 mM did not enhance the growth of tomato hypocotyls segments induced by 2,4-D or brassinolide, but sucrose at a concentration of 15 mM or higher in the presence of 2,4-D or brassinolide induced expansin gene LeEXPA2 transcript, member of a multigene family of extracellular proteins that mediate cell wall extension and relaxation during growth. Cui et al (2010) showed that high content of sucrose in adventitious root cultures of Hipericum perforatum resulted in osmotic stress and, in turn, induced the accumulation of secondary metabolites.…”

Section: Effect Of Soluble Sugars On Chlorophylls and Phenolic Compoumentioning

confidence: 99%

Effects of sugars on the growth and chlorophyll content in excised tulip stem in the presence of Indole-3-acetic acid

Wegrzynowicz-Lesiak

Saniewski

Góraj

et al. 2012

Journal of Fruit and Ornamental Plant Research

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The purpose of this study was to clarify the effect of sucrose on auxin-induced growth of stem excised from growing tulips and excised directly from cooled and not cooled bulbs, and on the growth of excised IV internode from growing plants in the presence of auxin. In all cases flower bud was replaced by IAA (indole-3- acetic acid, 0.1%, w/w in lanolin) and basal part of excised segments of stem was kept in distilled water or in solution of various sugars at different concentrations. IAA-induced growth of excised stems isolated from growing tulips was inhibited by sucrose at concentrations of 5.0% and 10.0%, but sucrose at 1.25% and 2.5% did not. Sucrose at all concentrations used evidently delayed senescence and increased chlorophyll contents in excised stems in the presence of IAA. Sucrose induced stiffing in isolated stems in the presence of IAA, and much less infective by pathogen in comparison to stem treated with IAA only. Mannitol and sorbitol at concentrations of 5.0% and 10.0% substantially inhibited IAA-induced growth of stem segments. Stem segments excised from cooled and not cooled tulip bulbs were more sensitive than those isolated from growing shoots due to application of sucrose and glucose; more inhibitory effect was observed. Sucrose at concentrations of 5.0% and 10.0% only slightly inhibited growth of IV internode treated with IAA and all concentrations of sucrose (1.25%, 2.5%, 5.0% and 10.0%) substantially increased chlorophyll content. The possible mode of actions of sucrose interacting with auxin to regulate stem growth is also discussed although sugar response is complicated by the fact that plants have multiple sugar-response pathways.

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“…Once inside, hexoses have a great capacity to stimulate specific sugar sensors (Koch 2004 and references therein) and the resulting signals can alter expression of diverse genes (Smeekens 2000;Rolland et al 2002;Sherson et al 2003). With their hydrolytic activity, therefore, invertases are potentially strong effectors of widely varying developmental processes LeClere et al 2008), including the biosynthesis and perception of auxins and abscisic acid (Koch 2004 and references therein; Arru et al 2008). Hormone sensing by cultured cells is a prerequisite for cell growth, which has restricted in the past a wider application of plant cell cultures (Chang and Sim 1995).…”

Section: Introductionmentioning

confidence: 99%

Sugar metabolism ofVetiveria zizanioidescells cultured with Amberlite XAD-4. Uptake of radiolabelled sugars, cell wall invertase activity and gene expression

Mucciarelli¹,

Bertea

Camusso³

2009

Journal of Plant Interactions

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Activated and non-activated Amberlite XAD-4 was added to the cell cultures of vetiver (Vetiveria zizanioides Stapf.) in order to study its effects as abiotic growth promoter. Amberlite XAD-4 increased vetiver cell viability, especially when used in the activated form. Results from labelling experiments in cell suspensions showed that XAD-4 prompted a significant decrease of sucrose Km, accompanied by an increased Vmax. Glucose uptake from the medium was almost doubled, as demonstrated by the increase of the radiolabelled fraction (25-fold the controls) in vetiver cells cultured with XAD-4. Affinity for fructose uptake by cells was not altered by the use of activated XAD-4, even though its uptake and Vmax increased. These results were further supported by a significant 7.5-fold increase in cell wall invertases (CWI) activity in XAD-4 elicited cells. Vetiver CWI gene expression was characterized by transcript RT-PCR analysis. The identity of the transcripts was confirmed by sequencing analysis. These results indicated the use of XAD-4 as an effective tool for vetiver cell biomass optimization.

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Effect of sugars on auxin-mediated LeEXPA2 gene expression

Cited by 7 publications

References 76 publications

New Insights on Plant Cell Elongation: A Role for Acetylcholine

New Insights on Plant Cell Elongation: A Role for Acetylcholine

Effects of sugars on the growth and chlorophyll content in excised tulip stem in the presence of Indole-3-acetic acid

Sugar metabolism ofVetiveria zizanioidescells cultured with Amberlite XAD-4. Uptake of radiolabelled sugars, cell wall invertase activity and gene expression

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