1983
DOI: 10.1128/jcm.18.3.658-662.1983
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Effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage

Abstract: Cytomegalovirus and varicella-zoster virus recovery from sucrose phosphate (0.2 M SP) and 70% sorbitol (sorbitol) was compared after storage at -70, 4, and 20 degrees C over time. Recovery from 0.2 M SP was uniformly better. More tissue culture infective doses and infectious foci were recovered in cell monolayers inoculated with 0.2 M SP virus stocks as compared with viruses stored in 70% sorbitol. Although both viruses were isolated from diluted fresh stocks (10(-1) through 10(-4], freezing diluted virus susp… Show more

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Cited by 37 publications
(16 citation statements)
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“…Although sorbitol has been recommended for the transport and holding of CMV and other enveloped viruses, titers may decrease rapidly. Indeed, CMV and VZV infectivity was lost when these viruses were stored in sorbitol, even for short periods of time (33). Virus recovery was significantly better from 0.2 M SP.…”
Section: Liquid Mediamentioning
confidence: 99%
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“…Although sorbitol has been recommended for the transport and holding of CMV and other enveloped viruses, titers may decrease rapidly. Indeed, CMV and VZV infectivity was lost when these viruses were stored in sorbitol, even for short periods of time (33). Virus recovery was significantly better from 0.2 M SP.…”
Section: Liquid Mediamentioning
confidence: 99%
“…Buffered sorbitol and sucrose-based solutions. Sorbitol is a cryoprotectant and thus has been used both for the storage of viruses and as an additive in transport media (33). Among the more frequently isolated viruses are herpesviruses, and, of these, cytomegalovirus (CMV) and VZV are the most labile.…”
Section: Liquid Mediamentioning
confidence: 99%
See 1 more Smart Citation
“…On the other hand, the remarkable short-term stability of the stocks prepared in SF900II and Express Five, which is advantageously compared with the stability reported for stocks prepared in another commercial serum-free media, 10,12 as well as in UNL-10 supplemented with soybean oil emulsion, could be explained by the presence of sucrose. Sucrose has a direct stabilizing effect on enveloped animal viruses, 41,47,48 including also baculoviruses. 10,12,13 Besides, sucrose could also contribute indirectly to preserve the viral infectivity through the stabilization of the lipid emulsion structure.…”
Section: Discussionmentioning
confidence: 99%
“…Viral particles were concentrated by ultracentrifugation in an SW28 rotor (Beckman Coulter, Krefeld, Germany) at 22,000 rpm for 1 h at 4°C. Pellets were resuspended in a 1:1 mixture of medium and 0.2 M sucrose phosphate (84). Aliquots were stored at Ϫ80°C.…”
Section: Methodsmentioning
confidence: 99%