Effect of sucrose on nisin production in batch and fed‐batch culture by Lactococcus lactis

Abstract

The effects of sucrose on cell growth and nisin production by Lactococcus lactis were investigated in batch and pH feed-back controlled fed-batch cultures. In batch cultures, nisin titer reached its maximum, 2658 IU cm −3 , at the initial sucrose concentration of 30 g dm −3 . With sucrose concentrations higher than 30 g dm −3 , nisin production decreased while the biomass was not influenced significantly. By using the pH feed-back controlled method, residual sucrose concentration could be controlled well in fedbatch cultures and three conditions (sucrose maintained at 2, 16, 20 g dm −3 , respectively) were evaluated. Maintaining a low sucrose concentration at 2 g dm −3 during feeding favored nisin biosynthesis, and the maximum nisin titer obtained was 4961 IU cm −3 compared with 3370 IU cm −3 (16 g sucrose dm −3 )and 3498 IU cm −3 (20 g sucrose dm −3 ), respectively.

Production of a highly concentrated probiotic culture of Lactococcus lactis CECT 539 containing high amounts of nisin

“…As observed in the present work, both the biomass and nisin productions obtained in the fed-batch fermentations with the strains CECT 539 (Vázquez et al 2004), ATCC 11454 (Lv et al 2005;Papagianni et al 2007b), NIZO 22186 (Pongtharangkul andDemirci 2006) and W28 (Wu et al 2009) were also higher than the corresponding batch cultures.…”

“…This approach has the additional advantage of considerably reducing the initial nutrient (Pr, TN and TP) content of these types of wastes (DW, CW and CMPW) and could consequently serve as an appropriate method to reduce their contamination effect. Vázquez et al (2004) in a realkalized fed-batch culture on a synthetic medium containing glucose, six salts, five vitamins and nine amino acids c Results obtained by Lv et al (2005) in a fed-batch culture in a complex medium containing sucrose, yeast extract, soy peptone and salts d Results obtained by Papagianni et al (2007a, b) in a fed-batch glucostat culture in the synthetic MCD medium containing glucose, 18 amino acids and 14 vitamins among other ingredients e Results obtained by Pongtharangkul and Demirci (2006) in fed-batch culture at constant pH (6.8) in a complex medium containing sucrose, peptone, yeast extract and salts f Results obtained by Wu et al (2009) in a variable volume fed-batch fermentation at constant pH (6.8) in a fermentation medium containing corn syrup, sucrose, peptone, yeast extract and salts…”

Glycolysis and the regulation of glucose transport in Lactococcus lactis spp. lactis in batch and fed-batch culture

“…Since glucose is mainly converted to lactic acid by the homofermentative L. lactis , glucose concentration can be maintained at constant levels by pH feed-back controlled fed-batch culture (controlling the decrease of pH caused by lactic acid accumulation). The method has been described by Lv et al [46] and employed successfully in studies on the effect of sucrose on nisin production by L. lactis . The feed consisted of a glucose (150–300 g l -1 ) and NaOH (140 g l -1 ) solution that was added at a rate of 5 to 10 g l -1 h -1 , designed to keep the glucose concentration relatively constant, the variation not exceeding 5 %.…”

Variable Volume Fed-Batch Fermentation for Nisin Production by Lactococcus lactis subsp. lactis W28

“…De Vuyst and Vandamme [4] found that carbon source had an important influence on nisin biosynthesis due to the genetic link between sucrose metabolism and nisin production, and the regulation of carbon metabolism appeared to be a major control mechanism for nisin biosynthesis. Lv et al [5][6][7] investigated the effects of feeding of carbon source and nitrogen source on the biosynthesis of nisin and established a pH feedback-controlled fed-batch fermentation technique for nisin production. These studies focused more on the effects of the limitation of carbon and nitrogen sources on nisin biosynthesis, while variations in broth volume caused by feeding were generally ignored despite its importance in industrial production.…”