Effect of succinate on phosphate solubilization in nitrogen fixing bacteria harbouring chick pea and their effect on plant growth

Iyer, Bhagya; Rajput, Mahendrapal Singh; Rajkumar, Shalini

doi:10.1016/j.micres.2017.05.005

Cited by 40 publications

(20 citation statements)

References 42 publications

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“…The high amount of organic acids exudated from the host plant feed and control proximal microbiota consisting of plant growth-promoting microorganisms (PGPM) as well as pathogens, and the microbiota in rhizosphere may also affect the concentration of organic acids excreted from the plant host by modulating their regulatory genes ( 122 , 123 ). As organic acids can affect the growth and plant-promoting activities of PGPM, e.g., suppressing phosphate stabilization ( 124 ), the concentration of organic acids should be well fine-tuned by the host-microbe regulatory network ( 125 ).…”

Section: Microbial Metabolites: Beneficial and Deleterious Effects Ofmentioning

confidence: 99%

How Can We Define “Optimal Microbiota?”: A Comparative Review of Structure and Functions of Microbiota of Animals, Fish, and Plants in Agriculture

et al. 2018

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All multicellular organisms benefit from their own microbiota, which play important roles in maintaining the host nutritional health and immunity. Recently, the number of studies on the microbiota of animals, fish, and plants of economic importance is rapidly expanding and there are increasing expectations that productivity and sustainability in agricultural management can be improved by microbiota manipulation. However, optimizing microbiota is still a challenging task because of the lack of knowledge on the dominant microorganisms or significant variations between microbiota, reflecting sampling biases, different agricultural management as well as breeding backgrounds. To offer a more generalized view on microbiota in agriculture, which can be used for defining criteria of “optimal microbiota” as the goal of manipulation, we summarize here current knowledge on microbiota on animals, fish, and plants with emphasis on bacterial community structure and metabolic functions, and how microbiota can be affected by domestication, conventional agricultural practices, and use of antimicrobial agents. Finally, we discuss future tasks for defining “optimal microbiota,” which can improve host growth, nutrition, and immunity and reduce the use of antimicrobial agents in agriculture.

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Section: Microbial Metabolites: Beneficial and Deleterious Effects Ofmentioning

confidence: 99%

How Can We Define “Optimal Microbiota?”: A Comparative Review of Structure and Functions of Microbiota of Animals, Fish, and Plants in Agriculture

et al. 2018

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“…PSB improve N, P and K nutrition and may function as biocontrol agents of photopathogenic fungi, synthesizing phytohormones in the rhizophere, and as a result may promote plant growth and development. PSB play a vital role in P availability from both organic and mineral sources (Iyer and Rajkumar, 2017). This role in attributed to the ability of PSB to produce low molecular weight acids (Al- Enazy et al, 2017) such as formic, acetic, propionic, lactic, glycoliuc, fumaric and succinic acid (Rashid et al, 2004) which use their carboxyl and hydroxyl groups to chelate cation such as Ca +2 and Mg +2 .…”

Section: Dual Symbiosis Between Vam Andmentioning

confidence: 99%

Effect of Biofertilizers and Vesicular Arbuscular Mycorrhizae on Holy Basil (Ocimum sanctum)

Harishkumar¹,

Karishmaa²,

Meenaloshini³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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“…Phosphatases are widely distributed in nature from prokaryotes to higher eukaryotes excluding some higher plants. Various microorganisms including A. niger secrete phosphatases in a phosphate mediated repression process [6]. High phosphatase production is only observed under limiting amounts of phosphate in culture media.…”

Section: Isolation and Activity Determination Of Enzyme Phosphatase Smentioning

confidence: 99%

Isolation and Activity Determination of Enzyme Phosphatase Secreted by Aspergillus niger

Zharare

Mangoyi

2019

Int. Ann. Sci.

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The use of enzymes on industrial scale saves a lot of energy and avoids pollution, thus holding a promise for green and economically sustainable alternative strategies in industrial transformations. Generally, the fungi Aspergillus niger secretes enzymes which can be used in different industries. Thus, coming up with these enzymes in large amounts will definitely result in reduced costs encountered in importing them for industrial use. This study focussed on isolation and activity determination of an enzyme phosphatase secreted by Aspergillus niger. This enzyme can be of great importance in molecular biology industries, particularly for recombinant DNA technology. For this study, pure cultures of Aspergillus niger were used. Aspergillus niger was resuscitated on potato dextrose agar and then subcultured in Adam’s medium, a medium specific for the production of phosphatase. Cells were centrifuged and the filtrate was collected whilst the residue was discarded. The filtrate was expected to contain the crude enzyme phosphatase since Aspergillus niger secretes the extracellular enzyme into the medium. Disodium phenyl phosphate was used as a substrate for the determination of the phosphatase activity. The enzyme activity was determined spectrophotometrically by reading absorbance of phenol formed in the presence of enzyme and the substrate. The concentration of phenol liberated was then used to calculate the enzyme activity expressed in King Armstrong Units (KAU). Further work on enzyme activity determination was done by varying enzyme and substrate concentrations. Results showed that the isolated alkaline phosphatase had activity of 4.0 KAU and 4.5 KAU at 25 ºC and 37 ºC respectively. Acidic phosphatase had activity of 5 KAU and 7 KAU at 25 ºC and 37 ºC respectively. Rate of activity increased upon increasing enzyme concentration and substrate. Thus, Aspergillus niger produces the enzyme phosphatase, however, there is need to induce the production of these enzymes for industrial use.

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Effect of succinate on phosphate solubilization in nitrogen fixing bacteria harbouring chick pea and their effect on plant growth

Cited by 40 publications

References 42 publications

How Can We Define “Optimal Microbiota?”: A Comparative Review of Structure and Functions of Microbiota of Animals, Fish, and Plants in Agriculture

How Can We Define “Optimal Microbiota?”: A Comparative Review of Structure and Functions of Microbiota of Animals, Fish, and Plants in Agriculture

Effect of Biofertilizers and Vesicular Arbuscular Mycorrhizae on Holy Basil (Ocimum sanctum)

Isolation and Activity Determination of Enzyme Phosphatase Secreted by Aspergillus niger

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