Effect of substrates on growth and shelf life of Trichoderma harzianum and its use in biocontrol of diseases

Singh, Anand Kumar; Srivastava, S. C.; Singh, Harsh

doi:10.1016/j.biortech.2006.01.002

Cited by 57 publications

(55 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the current study, cornmeal, an important natural source of carbon and nitrogen with a relatively low cost, was shown to promote significantly the formation of SH2303-derived chlamydospores in liquid fermentation. Several other studies have reported on the use of alternative cheap raw materials for Trichoderma biomass production, including bran, and peanut hull meal (Singh et al, 2007;Maurya et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation

Song

et al. 2016

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:Trichoderma-based formulations are applied as commercial biocontrol agents for soil-borne plant pathogens. Chlamydospores are active propagules in Trichoderma spp., but their production is currently limited due to a lack of optimal liquid fermentation technology. In this study, we explored response surface methodologies for optimizing fermentation technology in Trichoderma SH2303. Our initial studies, using the Plackett-Burman design, identified cornmeal, glycerol, and initial pH levels as the most significant factors (P<0.05) for enhancing the production of chlamydospores. Subsequently, we applied the Box-Behnken design to study the interactions between, and optimal levels of, a number of factors in chlamydospore production. These statistically predicted results indicated that the highest number of chlamydospores (3.6×10 8 spores/ml) would be obtained under the following condition: corn flour 62.86 g/L, glycerol 7.54 ml/L, pH 4.17, and 6-d incubation in liquid fermentation. We validated these predicted values via three repeated experiments using the optimal culture and achieved maximum chlamydospores of 4.5×10 8 spores/ml, which approximately a 8-fold increase in the number of chlamydospores produced by T. harzianum SH2303 compared with that before optimization. These optimized values could help make chlamydospore production cost-efficient in the future development of novel biocontrol agents.

show abstract

Section: Discussionmentioning

confidence: 99%

Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation

Song

et al. 2016

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

show abstract

“…These bags were incubated at 25±2°C for 9 d and Trichoderma−colonized MSC was air-dried and ground to fine powder in a temperature controlled cool jacket grinder (Basco, India) at 20°C. The talcbased formulation for T-1055 was prepared by following the method described by Singh et al (2007). Briefly, 1.0 kg of talc powder (Basco, India) was taken in a sterilized metal tray and its pH was adjusted to neutral by adding calcium carbonate (CaCO 3 ; HiMedia, RM397) @ 15 g kg −1 .…”

Section: Mass Multiplication and Formulation Preparationmentioning

confidence: 99%

“…An example of BCAs are Trichoderma spp., which have been shown to induce local and systemic defence responses in cucumber (Segarra et al 2007;Viterbo et al 2005;Viterbo et al 2007;Yedidia et al 1999Yedidia et al , 2003 and other economically important agricultural crops, such as cotton, tobacco, lettuce, maize, chickpea and bell pepper (Ahmed et al 2000;Chen et al 2005;De Meyer et al 1998;Howell et al 2000;Singh et al 2007;Wilson et al 2008). Induced disease resistance is the phenomenon by which a plant mobilises its own defence mechanism to restrict disease development (Harman et al 2004).…”

Section: Introductionmentioning

confidence: 97%

Trichoderma harzianum- mediated reprogramming of oxidative stress response in root apoplast of sunflower enhances defence against Rhizoctonia solani

Singh

et al. 2011

Eur J Plant Pathol

View full text Add to dashboard Cite

Trichoderma harzianum is an effective biocontrol agent against the devastating plant pathogen Rhizoctonia solani. Despite its wide application in agriculture, the mechanisms of biocontrol are not yet fully understood. Mycoparasitism and antibiosis are suggested, but may not be sole cause of disease reduction. In the present study, we investigated the role of oxidant-antioxidant metabolites in the root apoplast of sunflower challenged by R. solani in the presence/absence of T. harzianum NBRI-1055. Analysis of oxidative stress response revealed a reduction in hydroxyl radical concentration ( • OH; 3.6 times) at 9 days after pathogen inoculation (dapi), superoxide anion radical concentration (O 2 •− ; 4.1 times) at 8 dapi and hydrogen peroxide concentration (H 2 O 2 ; 2.7 times) levels at 7 dapi in plants treated with spent maize-cob formulation of T. harzianum NBRI-1055 (MCFT), as compared to pathogen-inoculated plants. The protection afforded by the biocontrol agent was associated with the accumulation of the ROS gene network: the catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and ascorbate peroxidase (APx), maximum activity of CAT (11.0 times) was observed at 8 dapi, SOD (7.0 times) at 7 dapi, GPx (5.4 times) and APx (8.1 times) at 7 dapi in MCFT-treated plants challenged with the pathogen. This was further supported by the inhibition of lipid and protein oxidation in Trichoderma-inoculated plants.MCFT stimulated the accumulation of secondary metabolites of phenolic nature that increased up to five-fold and also exhibited strong antioxidant activity at 8 dapi, eventually leading to the systemic accumulation of phytoalexins. These results suggest that T. harzianum-mediated biocontrol may be related to alleviating R. solani-induced oxidative stress.

show abstract

“…Les Trichoderma sont très efficaces pour la lutte contre les maladies racinaires et aériennes des plantes mais aussi pour la dégradation de composés toxiques présents dans les sols Kamala et Indira Devi, 2012). La présence de Trichoderma dans le sol joue à la fois un rôle préventif et curatif (Harman et al, 2004;Singh et al, 2007). Les sols inoculés par ces champignons protègent les cultures et garantissent un milieu sain pour un développement normal de la végétation (Harman, 2000, Mouria et al, 2007.…”

Section: Introductionunclassified

Influence <i>in vitro</i> de divers facteurs abiotiques (température, pH, salinité) sur la croissance mycélienne de trois souches locales de <i>Trichoderma</i> sp.

Gueye¹,

Ndiaye²,

Sarr³

et al. 2016

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

Influence in vitro de divers facteurs abiotiques (température, pH, salinité) sur la croissance mycélienne de trois souches locales de Trichoderma sp. RESUMELes Trichoderma sont des champignons imparfaits saprophytes que l'on retrouve dans divers milieux comme le sol, le bois mort, les débris des végétaux et les organes aériens des plantes. Ils ont un rôle phytoprotecteur et stimulateur du développement des plantes associées. L'effet de différents facteurs abiotiques (température, pH, salinité) sur la croissance mycélienne de trois souches locales de Trichoderma a été évalué. Ces souches ont été isolées à partir de la rhizosphère de la tomate. Une étude préliminaire a été effectuée sur quatre milieux de culture (PDA, MEA, SB et DOX) pour la sélection du milieu d'étude. Les résultats obtenus montrent que le milieu PDA permet une meilleure croissance des souches de Trichoderma. La température optimale de croissance des différentes souches est de 29 °C et le pH 5 se révèle être le plus adéquat pour la culture des Trichoderma. Cette étude montre également que les souches de Trichoderma résistent à des concentrations de sel (NaCl) allant jusqu'à 3%. © 2016 International Formulae Group. All rights reserved. Mots clés:Trichoderma, facteurs limitant, température, pH, salinité, milieu de culture.In vitro influence of various abiotic factors (température, pH, salinity) on mycelium growth of three local strains of Trichoderma sp. ABSTRACTTrichoderma are saprophytic imperfect fungi that are found in various environments such as soil, dead wood, vegetable fragments and plant aerial organs. They protect and stimulate development of related plants. Effects of various abiotic factors (temperature, pH, salinity) on mycelium growth of three local Trichoderma strains were investigated. These strains were isolated from tomato rhizosphere. A preliminary study was performed on four culture media (PDA, MEA, SB and DOX) for the selection of the best media. The results N. GUEYE et al. / Int. J. Biol. Chem. Sci. 10(2): 769-778, 2016 770 showed that PDA medium was highly growth of Trichoderma strains. The optimum temperature for growth of different strains is 29 °C and pH 5 was found to be most suitable for Trichoderma culture. Indeed, Trichoderma strains resistant to salt concentrations (NaCl) up to 3%.

show abstract

Effect of substrates on growth and shelf life of Trichoderma harzianum and its use in biocontrol of diseases

Cited by 57 publications

References 12 publications

Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation

Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation

Trichoderma harzianum- mediated reprogramming of oxidative stress response in root apoplast of sunflower enhances defence against Rhizoctonia solani

Influence <i>in vitro</i> de divers facteurs abiotiques (température, pH, salinité) sur la croissance mycélienne de trois souches locales de <i>Trichoderma</i> sp.

Contact Info

Product

Resources

About