Abstract. We report on the structural properties of ZnO films grown on (001) Si, (001) GaAs, (0001) Al 2 O 3 substrates and a (0001) GaN template by RF-sputtering. It is observed that all the ZnO films have textured structures with a preferred orientation of the c-axis, irrespective growth conditions, in terms of atomic-force microscope and wide-range X-ray diffractometer. However, it is found that the ZnO films in the ZnO-on-Si and ZnO-on-GaAs are consisted of various domains with different orientations, while the ZnO films in the ZnO-on-Al 2 O 3 and ZnO-on-GaN are consisted of a single domain with the same orientation in terms of the M-scan of X-ray diffractometer. Moreover, it is found that the ZnO-onGaN has smaller edge dislocation density and screw dislocation density than the ZnO-on-Al 2 O 3 , which seriously depends on substrate temperature, in terms of the Z-scan of X-ray diffractometer.