Effect of substrate load on anaerobic fermentation of rice straw with rumen liquid as inoculum: Hydrolysis and acidogenesis efficiency, enzymatic activities and rumen bacterial community structure

Liang, Jinsong; Zhang, Haibo; Zhang, Panyue; Zhang, Guangming; Cai, Yajing; Wang, Qingyan; Zhou, Zeyan; Ding, Yiran; Zubair, Muhammad

doi:10.1016/j.wasman.2021.02.017

Cited by 44 publications

(11 citation statements)

References 52 publications

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“…The same genus has been reported to generate acetic and propionic acid under different pH conditions [39, 40]. Production of butyric and valeric acid by some genera of the Ruminococcaceae has been reported [41]. Under acidic pH, the Ruminococcaceae strain CPB6 was found to generate caproic acid [42].…”

Section: Resultsmentioning

confidence: 99%

Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis

Sarkar

Christakopoulos

Μάτσακας

2022

Engineering in Life Sciences

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The present study reports the mixed culture acidogenic production of biohydrogen and carboxylic acids (CA) from brewery spent grains (BSG) in the presence of high concentrations of cobalt, iron, nickel, and zinc. The metals enhanced biohydrogen output by 2.39 times along with CA biosynthesis by 1.73 times. Cobalt and iron promoted the acetate and butyrate pathways, leading to the accumulation of 5.14 gCOD/L of acetic and 11.36 gCOD/L of butyric acid. The production of solvents (ethanol + butanol) was higher with zinc (4.68 gCOD/L) and cobalt (4.45 gCOD/L). A combination of all four metals further enhanced CA accumulation to 42.98 gCOD/L, thus surpassing the benefits accrued from supplementation with individual metals. Additionally, 0.36 and 0.31 mol green ammonium were obtained from protein‐rich brewery spent grain upon supplementation with iron and cobalt, respectively. Metagenomic analysis revealed the high relative abundance of Firmicutes (>90%), of which 85.02% were Clostridium, in mixed metal‐containing reactors. Finally, a significant correlation of dehydrogenase activity with CA and biohydrogen evolution was observed upon metal addition.

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Section: Resultsmentioning

confidence: 99%

Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis

Sarkar

Christakopoulos

Μάτσακας

2022

Engineering in Life Sciences

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“…Bacteria at phylum, genus and species level, as well as fungi and viruses at genus level were analyzed based on species annotation results. At the phylum level of bacteria ( Figure 1B ), microbiota was mainly composed of Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria, among which Firmicutes and Bacteroidetes accounted for more than 90% of them ( 23 ). Bacteroidetes was a Gram-negative bacterial that plays an essential role in carbohydrate metabolism ( 24 ).…”

Section: Resultsmentioning

confidence: 99%

Wheat supplement with buckwheat affect gut microbiome composition and circulate short-chain fatty acids

Yao

et al. 2022

Front. Nutr.

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Buckwheat has beneficial effects on human intestinal health, which is often compounded with wheat to make food. Therefore, the effect of cereals mixture via in vitro fermentation on gut microbes and short-chain fatty acids (SCFAs) were investigated in this study. The mixture of wheat and tartary buckwheat (WT) produced more lactate and acetate, and the mixture of wheat and sweet buckwheat (WE) produced more propionate and butyrate. Compared with wheat (WA), the relative abundance of some beneficial bacteria significantly increased, such as Sutterella in WT and Faecalibacterium in WE. Cereals mixture also affected the expression of functional genes, involved in metabolic pathways and carbohydrate-active enzymes (CAZymes) that modulated SCFAs generation. This study provides new insights into the effects of sweet and tartary buckwheat on intestinal function, which is beneficial to applying both types of buckwheat in practical.

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“…Recent research has shown that lignocellulosic biomass is a large reserve of carbon, and it could be used as a substrate for bioenergy production ( Yamamoto et al, 2014 ; Nanda et al, 2015 ; Sawatdeenarunat et al, 2015 ). However, considerable amount of lignocellulosic biomass is simply discarded or burnt in the field, leading to resource wastage and environmental pollution ( Liang et al, 2021 ). Therefore, it has become increasingly important and urgent to rationally utilize lignocellulosic biomass.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic Fungi Isolated From Bactrian Camel Rumen Contents Have Strong Lignocellulosic Bioconversion Potential

Xue

Shen

et al. 2022

Front. Microbiol.

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This study aims to obtain anaerobic fungi from the rumen and fecal samples and investigates their potential for lignocellulosic bioconversion. Multiple anaerobic strains were isolated from rumen contents (CR1–CR21) and fecal samples (CF1–CF10) of Bactrian camel using the Hungate roll tube technique. After screening for fiber degradability, strains from rumen contents (Oontomycessp. CR2) and feces (Piromycessp. CF9) were compared withPecoramycessp. F1 (earlier isolated from goat rumen, having high CAZymes of GHs) for various fermentation and digestion parameters. The cultures were fermented with different substrates (reed, alfalfa stalk,Broussonetia papyriferaleaves, andMelilotus officinalis) at 39°C for 96 h. TheOontomycessp. CR2 had the highest total gas and hydrogen production from most substrates in thein vitrorumen fermentation system and also had the highest digestion of dry matter, neutral detergent fiber, acid detergent fiber, and cellulose present in most substrates used. The isolated strains provided higher amounts of metabolites such as lactate, formate, acetate, and ethanol in thein vitrorumen fermentation system for use in various industrial applications. The results illustrated that anaerobic fungi isolated from Bactrian camel rumen contents (Oontomycessp. CR2) have the highest lignocellulosic bioconversion potential, suggesting that the Bactrian camel rumen could be a good source for the isolation of anaerobic fungi for industrial applications.

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Effect of substrate load on anaerobic fermentation of rice straw with rumen liquid as inoculum: Hydrolysis and acidogenesis efficiency, enzymatic activities and rumen bacterial community structure

Cited by 44 publications

References 52 publications

Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis

Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis

Wheat supplement with buckwheat affect gut microbiome composition and circulate short-chain fatty acids

Anaerobic Fungi Isolated From Bactrian Camel Rumen Contents Have Strong Lignocellulosic Bioconversion Potential

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