Effect of substrate feeding on production of fructosyltransferase by Penicillium purpurogenum

Abstract

Penicillium purpurogenum was found to produce both extracellular and intracellular fructosyltransferase. The organism could also produce sucrose hydrolytic enzyme. Sucrose was found to be the best carbon source for fructosyltransferase production. Maximum intracellular and extracellular fructosyltransferase production was observed after 3 rd and 4 th day of cultivation, respectively. The enzyme activity was optimum at temperature of 55ºC and pH 5.5. The addition of amino acids, like leucine induced slightly the extracellular fructosyltransferase production, where as histidine and leucine had little inductive effect on intracellular fructosyltransferase production. Enhanced production of fructosyltransferase by Penicillium purpurogenum was observed when sucrose content was restored by additional sucrose feeding to the cultivation medium during production period.

Production and partial purification of α-amylase from Pseudomonas sp. 2 under solid-state fermentation

“…Dhake and Patil [15] reported that the addition of vitamins did not affect the enzyme xylanase production. Sapre et al [16] reported that vitamins had no influence on xylanase production by S. recemosum which is contradictory to our results.…”

Optimization of Medium Composition and Cultivation Parameters for Fructosyltransferase Production by Penicillium aurantiogriseum AUMC 5605

“…Its optimal concentration ranges from 4 g/L (W/V) (Sangeetha et al, 2005) to 5 g/L (W/V) (Vandáková et al, 2004;Lim et al, 2006), Mg 2+ affects the permeability of the cell wall for A. pullulans (Vandáková et al, 2004) and its optimal concentration varies from 0.3 g/L (W/V) (Sangeetha et al, 2005) to 2 g/L (W/V) (Balasubramaniem et al, 2001). NaNO 3 is the most common source of inorganic nitrogen in FTase production by some fungi and can be employed from 2 g/L (W/V) (Lim et al, 2006) to 25 g/L (W/V) (Dhake and Patil, 2007). The highest FTase production by Aureobasidium pululans was achieved using a culture medium containing (g/L): K 2 HPO 4 , 5; NaNO 3 , 10 and MgSO 4 ·7H 2 O, 0.5 (Vandáková et al, 2004).…”

“…The low enzyme activities observed at 100 and 150 rpm are most probably caused by poor oxygenation, despite the control of the dissolved oxygen concentration, especially at higher sucrose concentration (600 g/L) and also may be due to the inadequate mixing of the broth towards the later stages of growth affected the enzyme synthesis . When the culture medium was subjected to agitation, P. purpurogenum failed to grow and there was little fructosyltransferase production (Dhake and Patil, 2007). Agitation has been shown to influence enzyme production in many organisms.…”

“…Similarly, yeast extract was declared to be the best nitrogen source (Hayashi et al, 1992;Chen and Liu, 1996). Another common subject of investigation was the effect of minerals, mainly phosphorus, magnesium, iron, and calcium, on the FTase production and cell growth (Sangeetha et al, 2005;Dhake and Patil, 2007).…”

Novel agents for enzymatic and fungal hydrolysis of stevioside

“…Although the low yields of experiments for obtaining steviol (2), in some cases, recovery of stevioside (1) was high, implying that reaction yields can be improved, either by increasing the reaction time (27), altering chemical composition of the medium (24,35), changing a substrate feeding procedure (8) or by the use of reactors with controlled reaction conditions (18). On other hand, even methodologies leading to low yields of aglycons without side reactions are of huge interest since acidic conditions can not guarantee aglycon stability in some specific cases.…”