Effect of Substrate Conversion on Performance of Microbial Fuel Cells and Anodic Microbial Communities

Abstract

Performance of microbial fuel cells (MFCs) was monitored during the influent nutrient change from lactate to glucose/acetate/propionate and then to lactate. Meanwhile, anodic microbial communities were characterized by culture-independent molecular biotechnologies. Results showed MFC performance recovered rapidly when the lactate was replaced by one of its metabolic intermediates acetate, while it needed a longer time to recover if lactate substrate was converted to glucose/propionate or acetate to lactate. Secondary lactate feed enhanced the enrichment of bacterial populations dominating in first lactate feed. Electricity-producing bacteria, spp., and beneficial helpers, spp. and spp., revived from a low abundance as lactate secondary supply, but microbial communities were hard to achieve former profiles in structure and composition. Hence, microbial community profiles tended to recover when outside environmental condition were restored. Different substrates selected unique functional microbial populations.

Electricity generation and pollutants removal of landfill leachate by osmotic microbial fuel cells with different forward osmosis membranes

“…Pseudomonas [50], Arcobacter [51] and Rhodopseudomonas [52] are commonly found in bioelectrochemical systems and are speculated to be the main electrogenic bacteria in the OsMFCs. However, some well-known electroactive microorganisms (e.g., Anaeromusa [53], Dechlormonas, Geobacter [54], and etc.) were been found in this study, probably because the salt in the catholyte passed through the membranes and caused high anode salinity.…”

Electricity generation and pollutants removal of landfill leachate by osmotic microbial fuel cells with different forward osmosis membranes

“…Pseudomonas [60], Arcobacter [61] and Rhodopseudomonas [62] are commonly found in bioelectrochemical systems and are speculated to be the main electrogenic bacteria in the OsMFCs. However, some well-known electroactive microorganisms (e.g., Anaeromusa [63], Dechlormonas, Geobacter [64], and etc.) were not found in this study, probably because the salt in the catholyte passed through the membranes and caused high anode salinity.…”

Isolation and electrochemical analysis of a facultative anaerobic electrogenic strainKlebsiellasp. SQ-1

“…In contrast, using simple substrates as carbon sources such as acetate, formate, citrate, and pyruvate (Yousaf 2017; Zhao et al 2017; Zhou et al 2016), pure culture achieved better performance than mixed culture. Different types of electricigens like Geobacter sp., Shewanella sp., Pseudomonas sp., Citrobacter sp., Klebsiella sp., Escherichia sp., Bacillus sp., and Saccharomyces cerevisiae have been employed as biocatalysts in MFC (Bond et al 2003; Cao et al 2019; Ieropoulos et al 2005; Jimenez Pacheco et al 2023; Kim et al 2005; Rabaey and Verstraete 2005).…”