Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae

Abstract

In order to develop processing methods with high physiological activity for Protaetia brevitarsis larvae (PBL), subcritical water (SCW) extraction was applied. The dried powder (1 g) of PBL was extracted with 10 mL distilled water at 100, 200, and 300 °C for 30 min. The SCW treatment significantly (p \ 0.05) increased some physiological activities of the PBL extracts. The SCW extract at 300 °C increased alcohol dehydrogenase, acetaldehyde dehydrogenase, and tyrosinase inhibitory activities from 192.3 ± 4.1% to 452.2 ± 0.5%, 125.4 ± 2.9% to 153.3 ± 0.4%, and -7.0 ± 0.7% to 26.1 ± 1.4%, respectively, compared to the extract at 100 °C. Contrarily, the inhibition activity of angiotensin converting enzyme was the highest at 200 °C. These results suggest that SCW is a suitable method to extract and maintain the physiological activity of PBL.

Fermented Protaetia brevitarsis Larvae Ameliorates Chronic Ethanol-Induced Hepatotoxicity in Mice via AMPK and TLR-4/TGF-β1 Pathways

“…Therefore, it seems that cysteine contained in FPB can improve liver toxicity caused by acetaldehyde. In previous in vitro study, it was confirmed that P. brevitarsis larvae extract increased the activities of ADH and ALDH [ 41 ]. Moreover, in a prior study it was reported that leucine can accelerate ethanol elimination after acute ethanol administration by enhancing the activity of alcohol metabolizing enzymes such as ADH and ALDH in rats [ 42 ].…”

Composition analysis and tyrosinase inhibitory activity of Cinnamomum cassia Presl leaf hydrosol and Cymbopogon citratus ( DC .) Stapf leaf hydrosol

“…Detection of the inhibition of mushroom tyrosinase by CCPH, CCSH, cinnamaldehyde, and citral was based on the procedures described by Bae and Lee (2020) and Ho et al (2019) with slight modifications. Different concentrations of hydrosol samples were prepared by mixing with reverse osmosis water, and different concentrations of cinnamaldehyde and citral were prepared using ethanol.…”

“…Detection of the inhibition of mushroom tyrosinase by CCPH, CCSH, cinnamaldehyde, and citral was based on the procedures described by Bae and Lee (2020) and Ho et al (2019) with slight modifications.…”