Effect of subclinical intramammary infection on somatic cell counts, NAGase activity and gross composition of goats' milk

Leitner, Gabriel; Merin, Uzi; Silanikove, Nissim; Ezra, E.; Chaffer, M.; Gollop, Natan; Winkler, M.; Glickman, A.; Saran, A.

doi:10.1017/s0022029904000305

Cited by 58 publications

(53 citation statements)

References 25 publications

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“…Our results are also consistent with previous studies showing that LPS challenge induces significant casein degradation and identifies milk PL as a major cause for this (Moussaoui et al, 2002;Leitner et al, 2004aLeitner et al, , 2004bLeitner et al, and 2004cSilanikove et al, 2006;Rossi et al, 2009). The biological implications of these findings have been discussed before Silanikove et al, 2006) and, therefore, these aspects are not discussed here in detail.…”

Section: Lps Challenge Is Reflected By Accumulation Of Inflammatory Isupporting

confidence: 93%

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Silanikove

Rauch-Cohen

Shapiro

et al. 2012

Animal

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The aim of this work was to study the effects of mastitis induced by intramammary lipopolysaccharide (LPS) challenge on milk oxidative stability, as well as to understand the underlying biochemical processes that cause such changes. LPS challenge was associated with nitric oxide burst from the surrounding mammary epithelial cells and consequently induced nitrosative stress that was induced by the formation of NO 2 d from nitrite by lactoperoxidase. This response was associated with an , 3-fold increased formation of hazardous compounds: nitrotyrosines, carbonyls and lipid peroxides. We sustained the involvement of xanthine oxidase as a major source of hydrogen peroxide. In consistent with previous findings, catalase has been shown to play a major role in modulating the nitrosative stress by oxidizing nitrite to nitrate. The current hygienic quality criteria cannot detect mixing of low-quality milk, such as milk with high somatic cells, and nitrite with high-quality milk. Thus, development of an improved quality control methodology may be important for the production of high-quality milk.Keywords: milk, cow, mastitis, NO and NO 2 d, plasmin system Implication In this study, we show that intramammary treatment with lipopolysaccharide induced nitric oxide burst that was associated with nitrosative stress that reflected the formation of hazardous compounds in milk: nitrotyrosines, carbonyls and lipid peroxides. These changes were associated with impairment in milk quality. Currently, very little is known about the presence of oxidative substances in commercial milk and their industrial and health implications. As mastitis is a prevalent problem in the dairy industry, this study stresses the importance of gaining further information on the effect of different forms of mastitis on milk composition.

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Section: Lps Challenge Is Reflected By Accumulation Of Inflammatory Isupporting

confidence: 93%

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Silanikove

Rauch-Cohen

Shapiro

et al. 2012

Animal

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“…These results regarding the distribution of pathogens correspond well with other studies on subclinical mastitis infection in dairy goats throughout the world (Ndegwa et al. 2001;Leitner et al. 2004), in which coagulase negative Staphylococci are the predominant organisms isolated from bacteriological positive milk samples.…”

Section: Discussionsupporting

confidence: 80%

Epidemiological studies on subclinical mastitis in dairy goats in northern regions of Bangladesh

Begum

Hossain

Ershaduzzaman

et al. 2016

Bangladesh J. of Livestock Res.

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A cross sectional study was carried out from June, 2008 to December, 2010 to estimate the prevalence of subclinical mastitis and to determine the responsible bacterial pathogens in lactating dairy goats in northern districts of Bangladesh. A total of 292 goats and 584 udder halves milk samples were screened using California Mastitis Test (CMT). Prevalence of subclinical mastitis at goat level was 56.2% (164/292), and in udder half level it was 33.9% (198/584). The subclinical mastitis prevalence at goat level was high (71.6%) in Jamnapari goats as compared to Black Bengal goats (50.2%), whereas at the udder half level, subclinical mastitis prevalence was 45.1% and 29.6% in Jamnapari and Black Bengal goats, respectively. The pathogens isolated from subclinical mastitic milk samples were coagulase negative Staphylococci, Coliforms, Streptococcus spp., Staphylococcus aureus, Micrococcus spp., Pseudomonas aeruginosa and Bacillus cereus. Among these, the most frequent isolates were coagulase negative Staphylococci (28.8%), Coliforms (22.7%) and Streptococcus spp (15.2%). Out of four potential host related risk factors considered, litter size and body condition of goats were found to influence the prevalence rate of subclinical mastitis in goat significantly (p< 0.05). On the other hand, the subclinical mastitis was very significantly (p=0.0001) associated with the housing system of goats, i.e., goats reared in raised floor had a low subclinical mastitis infection rate (35.8%) as compared to reared in earth floor (62.2%). Antibiogram studies were also performed for the bacterial isolates and Gentamicin was found to be the most effective drug.

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“…Normal SCC is 68,000 cells·mL −1 in cows milk (Djabri et al 2002), around 75,000 cells·mL −1 in ewes milk (Ariznabarreta et al 2002;Gonzalo et al 2002) and varies between 210,000 and 1,120,000 cells·mL −1 in goats milk (Leitner et al 2004c;Manser 1986). A meta-analysis of the data concerning the rise in SCC during mastitis infection in cows has actually shown differences depending on the pathogen involved.…”

Section: Introductionmentioning

confidence: 93%

Mastitis impact on technological properties of milk and quality of milk products—a review

Maréchal

Thiéry²,

Vautor

et al. 2011

Dairy Science & Technol.

148

139

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The consequences of mastitis on the technological properties of milk and on the quality of milk products are widely reported in the literature. Besides, recent advances have shed light on the mechanisms involved in the udder response and subsequent milk changes in mastitis cases. This review gives an update on the literature regarding the impact of mastitis on milk composition and processing properties and collates recent data regarding the mechanisms involved in mastitis effects. It is an attempt to link field observations and experimental studies in order to better understand how mastites affect so dramatically the technological properties of milk. Both bovine and small ruminant milks are considered and a special emphasis is given on the role of staphylococci, streptococci, and Escherichia coli, the most common causative agents of mastitis.

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Effect of subclinical intramammary infection on somatic cell counts, NAGase activity and gross composition of goats' milk

Cited by 58 publications

References 25 publications

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Epidemiological studies on subclinical mastitis in dairy goats in northern regions of Bangladesh

Mastitis impact on technological properties of milk and quality of milk products—a review

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