Effect of styrylpyrone derivative (SPD) and SPD/foscarnet combination towards virus infected cell

Ibrahim, Nazlina; Shahar, Saleha; Wahab, Noor Zarina Abdul; Nor, Norefrina Shafinaz Md

doi:10.1063/1.5111241

Cited by 6 publications

(5 citation statements)

References 10 publications

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“…This structure becomes the core skeleton for other types of styryl-lactones such as furano-pyrones and pyrano-pyrones [ 30 ]. This phytochemical compound has been reported to have potent anti-viral activity against herpes simplex virus type 1 [ 31 , 32 ], an anti-cancer effect, and neurotoxicity [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

Wahab

Ibrahim

2022

Molecules

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A study was conducted to investigate the anti-viral effect of a styrylpyrone derivative (SPD) called goniothalamin and the effects on the dengue virus serotype 2 (DENV-2) replication cycle. The SPD was prepared from the root of Goniothalamus umbrosus after purification with petroleum ether. The isolated SPD was then subjected to gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses for structure validation. The cytotoxicity of the SPD was evaluated using a cell viability assay, while the anti-viral activity of the SPD towards DENV-2 was confirmed by conducting a foci reduction assay which involved virus yield reduction, time-of-addition, and time removal assays. Transcriptomic analysis via quantitative real-time polymerase chain reaction (qRT-PCR) using the DENV-2 E gene was conducted to investigate the level of gene transcript. Immunocytochemistry analysis was used to investigate the effects of SPD treatment on protein E expression. Finally, software molecular docking of the SPD and E protein was also performed. The cytotoxicity assay confirmed that the SPD was not toxic to Vero cells, even at the highest concentration tested. In the time-of-addition assay, more than 80% foci reduction was observed when SPDs were administered at 2 h post-infection (hpi), and the reduction percentage then dropped with the delay of the treatment time, suggesting the inhibition of the early replication cycle. However, the time removal assay showed that more than 80% reduction could only be observed after 96 h post-treatment with the SPD. Treatment with the SPD reduced the progeny infectivity when treated for 24 h and was dose-dependent. The result showed that transcript level of the E gene in infected cells treated with the SPD was reduced compared to infected cells without treatment. In immunocytochemistry analysis, the DENV-2 E protein exhibited similar expression trends, shown by the gene transcription level. Molecular docking showed that the SPD can interact with E protein through hydrogen bonds and other interactions. Overall, this study showed that SPDs have the potential to be anti-DENV-2 via a reduction in viral progeny infectivity and a reduction in the expression of the DENV-2 E gene and protein at different phases of viral replication. SPDs should be further researched to be developed into an effective anti-viral treatment, particularly for early-phase dengue viral infection.

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Section: Introductionmentioning

confidence: 99%

“…has been reported to have potent anti-viral activity against herpes simplex virus type 1 [31,32], an anti-cancer effect, and neurotoxicity [33]. The identification of a pure compound can be determined using gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR).…”

Section: Introductionmentioning

confidence: 99%

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

Wahab

Ibrahim

2022

Molecules

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“…In recent times, bioactive compounds derived from natural products such as plants, microorganisms, marine organisms, fungi, and animal are being explored with great interest as potential antiherpetic agents [7]. Plant-derived bioactive compounds have been shown to be able to elicit potent antiviral effects against HSV at multiple phases of viral replication including latency stage [8][9][10][11]. Besides that, natural botanical products are less cytotoxic toward mammalian cells, produce less undesired secondary effects, and are cheap in terms of production [12].…”

Section: Introductionmentioning

confidence: 99%

In vitro virucidal activity of Kyllinga nemoralis aqueous extract against herpes simplex virus

Wahab¹,

Ganasen²,

Rahman³

et al. 2022

J App Biol Biotech

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Kyllinga nemoralis is an ignored plant distributed in tropical, subtropical, and warm temperature regions around the world. K. nemoralis plant is rich with bioactive molecules exhibiting antiherpetic properties. This study determined the antiviral activities of K. nemoralis stem with leaves aqueous extract against herpes simplex virus type 1 (HSV-1). The stem with leaves of K. nemoralis plant was extracted using aqueous extraction method. The cytotoxicity of K. nemoralis extract was evaluated using a cell viability assay. Plaque reduction assays were carried out to estimate the antiviral activity of K. nemoralis extract against HSV-1. Antiviral studies consisted of post-treatment, pre-treatment, and virucidal assay. Cell viability assay revealed K. nemoralis stem with leaves aqueous extract is non-toxic toward Vero cells. K. nemoralis stem with leaves aqueous extract inhibited HSV-1 replication in Vero cells with 50% effective concentration (EC 50 ) = 0.004 mg/mL and selective index (SI) = 14 when given after adsorption to the cells. The EC 50 against HSV-1 was 0.013 mg/mL and SI = 4.31 when cells were treated 24 h before virus infection. K. nemoralis stem with leaves aqueous extract exhibited direct virucidal activity against HSV-1 with EC 50 = 0.0065 mg/mL and SI = 8.62. The results presented the potential of K. nemoralis as an anti-HSV-1 agent by the following modes: direct damage to viral particle, interruption of virus attachment, and suppression of viral replication cycle.

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“…This structure becomes the core skeleton for other types of styryl-lactones such as furanowww.videleaf.com pyrones and pyrano-pyrones [30]. This phytochemical compound has been reported to have potent anti-viral activity against herpes simplex virus type 1 [31,32], an anti-cancer effect, and neurotoxicity [33]. The identification of a pure compound can be determined using gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR).…”

Section: Introductionmentioning

confidence: 99%

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

Abd Wahab,

Ibrahim

2022

Prime Archives in Molecular Sciences

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Effect of styrylpyrone derivative (SPD) and SPD/foscarnet combination towards virus infected cell

Cited by 6 publications

References 10 publications

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

In vitro virucidal activity of Kyllinga nemoralis aqueous extract against herpes simplex virus

Styrylpyrone Derivative (SPD) Extracted from Goniothalamus umbrosus Binds to Dengue Virus Serotype-2 Envelope Protein and Inhibits Early Stage of Virus Replication

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