Effect of strontium doping on the biocompatibility of calcium phosphate-coated titanium substrates

Abstract

Background: Titanium biomedical devices coated with strontium-doped calcium phosphate ceramics can support desirable bone regeneration through anabolic and anti-catabolic effects of strontium and the compositions close to that of natural mineral tissue. Methods: Strontium was doped into the calcium phosphate coating using the cyclic pre-calcification method on the anodized titanium plate. The effects of the different concentration of strontium in treatment solution and cycle numbers of the pre-calcification treatment on the biocompatibility were investigated in terms of the changes in morphology and chemical composition of coating, ion release pattern and cytocompatibility in vitro. Results: At a high substitution ratio of strontium in the calcium phosphate coating, the size of precipitated particles was decreased and the solubility of the coating was increased. ASH55 group, which was coated by pre-calcification treatment of 20 cycles in coating solution with Sr:Ca molar ratio of 5:5, exhibited superior cellular attachment at 1 day and proliferation after 7 days of culturing in comparison with the non-doped surface and other doped surfaces. Conclusion: Sufficient strontium doping concentrations in calcium phosphate coating can enhance cell adhesion and proliferation on the titanium biomedical devices for bone regeneration.

Improved Osteogenesis of Selective-Laser-Melted Titanium Alloy by Coating Strontium-Doped Phosphate With High-Efficiency Air-Plasma Treatment

“…With regard to biocompatibility, the cell proliferation results showed excellent cell growth capability on Sr-CaP-p. It is worth noting that the enhancement of cell proliferation in the CaP-p group is not significant, which is consistent with some reports (Klymov et al, 2016;Nguyen et al, 2019). Confocal microscopy images showed that cells in the CaP-p group spread well with clear microfilaments while cells in the Sr-CaP-p group had a spindleshaped cell morphology with actively-dividing cells.…”

Evaluation of osteogenic and antibacterial properties of strontium/silver‐containing porous TiO2 coatings prepared by micro‐arc oxidation

“…Surface modification is the most effective way to improve biological properties of titanium implants and many inorganic elements are successfully incorporated into titanium coatings ( 4,6,9 ; 11,16 ). Within the elements, Sr exhibits excellent osteogenic potential 4,6,9,14‐16 and Ag shows strong antibacterial capability 3,4,6 . Both elements are successfully incorporated into titanium coatings either singly 9,14‐16 or in combination, 4 but in duplex techniques, which weakens the comprehensive biological properties of coatings and is not convenient for coating preparation.…”

“…Within the elements, Sr exhibits excellent osteogenic potential 4,6,9,14‐16 and Ag shows strong antibacterial capability 3,4,6 . Both elements are successfully incorporated into titanium coatings either singly 9,14‐16 or in combination, 4 but in duplex techniques, which weakens the comprehensive biological properties of coatings and is not convenient for coating preparation. Furthermore, short‐term osteogenic and antibacterial capability of these coatings are well documented, 3,6,14,15 but their long‐term effects remain less explored.…”

“…Numerous studies suggest that strontium (Sr) is the most valuable element for titanium coatings to stimulate bone formation. 3,4,15,16 Sr has been incorporated into many titanium coatings including hydroxyapatites 3,4,15 calcium phosphate, 15 titania nanotubes 16 and porous TiO 2,4 and desirable results have been obtained. Furthermore, Sr may partly compensate cytotoxity of excessive Ag and facilitate bone formation.…”

Preparation of Calcium Phosphate Compounds on Zirconia Surfaces for Dental Implant Applications

“…Soaking is a low-temperature method that is applicable to substrates of any shape while allowing for the formation of bands with growth factors, antibiotics, etc. [ 8 ].…”