Effect of streptozotocin-induced diabetes mellitus on alveolar bone deposition in the rat

Mishima, Naoyuki; Sahara, Noriyuki; Shirakawa, Masayori; Ozawa, Hiroki

doi:10.1016/s0003-9969(02)00152-8

Cited by 55 publications

(66 citation statements)

References 24 publications

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“…Diabetes induction was confirmed in rats 2 19 and 3 21 days after STZ injection. In the present study, day 5 was selected as the cut-off day.…”

Section: Discussionmentioning

confidence: 80%

“…They were fed with a standard laboratory diet in powder form, 18 given tap water ad libitum and acclimatized to the new environment for 7 days. 19 The study protocol was in accordance with local laws and regulations, with the European Communities Council Directive of 24 November 1986 (86/609/ EEC), and with guidelines approved by the Council of the American Psychological Society (1980). On day 1, the rats were anesthetized with an intramuscular injection of ketamine hydrochloride solution (100 mg/kg body weight, Imalgene  1000, MERIAL, Lyon, France) and xylazine (10 mg/kg body weight, Rompun  , Bayer HealthCare, Leverkusen, Germany).…”

Section: Methodology Animalsmentioning

confidence: 99%

“…Type 1 diabetes was induced by the intravenous injection (into the tail vein) of streptozotocin (STZ) (Sigma, St. Louis, USA), 45 mg/kg body weight, 20 dissolved in citrate buffer (10 mM, pH 4.5). Rats with glucose levels > 300 mg/dL 19,21 up to the 5 th day were included in the study. Body weight and blood glucose were measured daily 19 (glucometer Wellion  Linus, AgaMatrix Inc., Salem, USA) after a 12-hour nocturnal fast.…”

Section: Methodology Animalsmentioning

confidence: 99%

“…Rats with glucose levels > 300 mg/dL 19,21 up to the 5 th day were included in the study. Body weight and blood glucose were measured daily 19 (glucometer Wellion  Linus, AgaMatrix Inc., Salem, USA) after a 12-hour nocturnal fast. The induction of diabetes was confirmed when glucose levels first exceeded 300 mg/dL during the first 5 days (G d ).…”

Section: Methodology Animalsmentioning

confidence: 99%

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Adiponectin and interleukin-6 levels in insulin-treated diabetic rats with experimental periodontitis

et al. 2012

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The aim of the study was to compare the serum levels of adiponectin and interleukin-6 (IL-6) in insulin-treated diabetic rats with or without periodontitis. Forty male Wistar rats were randomly divided into 2 groups (20 rats each): a) insulin-treated diabetic group (control, DI) and b) insulin-treated diabetic periodontitis group (test, DIP). Diabetes was induced, and insulin treatment was initiated on day 5. On day 16, periodontitis was induced in the DIP group. All rats were euthanized on day 77. Adiponectin and IL-6 were assessed on days 16 and 77. At the end of the experiment, 14 and 11 rats survived in the DI and DIP groups, respectively. Adiponectin levels were statistically significantly higher at the end of the experiment compared with levels on day 16 in the periodontitis group (p < 0.05), but not in the control group. At the end of the experiment, adiponectin levels were statistically significantly higher in the periodontitis group compared with the control group (p < 0.05). Within-group and between-group comparisons of IL-6 levels showed no statistically significant difference. In conclusion, serum adiponectin was increased in insulin-treated diabetic rats with periodontitis in comparison with insulin-treated diabetic rats, while IL-6 levels did not differ between groups.

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“…Diabetes induction was confirmed in rats 2 19 and 3 21 days after STZ injection. In the present study, day 5 was selected as the cut-off day.…”

Section: Discussionmentioning

confidence: 80%

Section: Methodology Animalsmentioning

confidence: 99%

Section: Methodology Animalsmentioning

confidence: 99%

Section: Methodology Animalsmentioning

confidence: 99%

See 2 more Smart Citations

Adiponectin and interleukin-6 levels in insulin-treated diabetic rats with experimental periodontitis

et al. 2012

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“…3 Deneysel çalışmalar, diyabete bağlı görülen periodontal doku kaybına neden olabilecek bazı mekanizmalardan söz etmektedir. Diyabetin inflamatuar yanıtı uzatarak şiddetini artırdı-ğı, 4 monosit ve makrofajlarının fonksiyonlarını değişti-rerek periodontal patojenlere karşı daha fazla sitokin sentezine sebep olduğu 5 ve alveolar kemiğin turnoverını etkilediği 6 gösterilmiştir. Özet olarak kontrolsüz diyabette görülen tüm bu değişiklikler, diyabetin varlı-ğında görülen periodontal doku kaybında artışa neden olmaktadır.…”

Section: Gġrġġunclassified

Yeni̇ Ti̇p 1 Di̇abetes Melli̇tus Teşhi̇si̇ Konulan Çocuklarin Beslenme Ve Ağiz Hi̇jyeni̇ Eği̇ti̇mi̇ni̇n Değerlendi̇ri̇lmesi̇

Aral¹,

Demır²

2016

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Literatürde tip 1 diyabetin erken döneminde verilen beslenme ve ağız hijyeni eğitiminin sonuçlarını değerlendiren çalışma bulunmamaktadır. Bu çalışmanın amacı Tip 1 Diabetes Mellitus (T1DM) teşhisi konulan çocuklarda beslenme ve ağız hijyeni alışkanlıklarını incelemek, aynı yaş grubu sistemik sağlıklı kontrollerle karşılaştırmak ve bulguları diyabetin kontrolünün 3. ayında tekrar değerlendirmektir. Gereç ve Yöntem: Çalışmamıza 10-16 yaş arası 60 birey dâhil edildi. Yaş, boy, kilo ve vücut kütle indeksi açısından benzer olan çalışma gruplarımızı; sistemik olarak sağlıklı bireyler (S), yeni T1DM teşhisi konulan hastalar (DY) ve T1DM"li hastaların 3. ay kontrolleri (DK) oluşturdu. Bireylerin periodontal muayenelerinde OHİ-S (Oral Hijyen İndeksi) ve SKI (Sulkus Kanama İndeksi) belirlendi. Çalışmaya katılan bireylerin ebeveynleri ile beslenme ve ağız hijyeni anketi yapıldı. Bulgular: DY grubunda OHİ-S değerleri, DK grubuna göre daha yüksekti (p=0.005). DY grubunda SKİ değerleri, S ile DK gruplarından daha fazla bulundu (Sırasıyla; p = 0.002 ve p=0.015). Öğün atlamayan bireylerin yüzdesi DK grubunda en yüksekti (%90). DY grubunda; genellikle tatlı tüketen bireylerin yüzdesi (%66.7) ve genellikle içecek tüketen bireylerin yüzdesi (%76.7) en yüksekti. S grubunda genellikle atıştırmalık tüketen bireylerin yüzdesi en düşüktü(%26.7). Genellikle dişlerini fırçalayan ve düzenli gargara yapan bireylerin yüzdeleri DY grubuna göre (Sırasıyla; %30, %10), DK grubunda (Sırasıyla; %76.7, %46.7) yükseldiği izlendi. DY grubunda fırçalama esnasında genellikle dişeti kanaması olan bireylerin yüzdesi (%53.3), S (%26.7) ve DK (%23.3) gruplarına göre daha fazla bulundu. Sonuçlar: Ağız hijyenini de etkileyebilen beslenme alışkanlıkları, kontrolsüz T1DM"li çocuklarda bozulduğu izlendi. Dolayısı ile diyabetik bireylere verilen ağız hijyeni ve beslenme eğitimi; hem diyabetin kontrolü, hem de periodontal sağlığın idamesi için önemlidir.

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Insulin stimulates osteoblast proliferation and differentiation through ERK and PI3K in MG‐63 cells

Yang¹,

Zhang²,

Wang³

et al. 2010

Cell Biochemistry & Function

129

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Insulin has been proposed to be an anabolic agent in bone, but the mechanisms underlying insulin effects on osteoblast differentiation are still not clear. To explore the mechanisms of action of insulin on osteoblast growth and differentiation, human osteoblastic cell line-MG-63 was used and stimulated by insulin in the presence or absence of ERK inhibitor PD98059, PI3-K inhibitor LY294002, or inhibitor PD98059 + LY294002. The results showed that insulin positively regulated the expression of its receptor. Insulin stimulated the proliferation of MG-63 cells in a time- and dose-dependent manner and blockade of both MAPK and PI3K pathways could inhibit the cell proliferation. In addition, ALP activity, the secretion of type I collagen, OC gene expression, and mineralized nodule formation were increased in the insulin treated group, whereas these indicators were decreased after treatment with blocking agents. However, treatment with PI3-K inhibitor LY294002 significantly reversed the down-regulation of Runx2 expression and treatment with ERK inhibitor PD98059 remarkably decreased up-regulation of Osx and IGF-1 expression after insulin treatment. Therefore, the data obtained from this study suggested that insulin promoted osteoblast proliferation and differentiation through MAPK and PI3K pathway in MG-63 cells.

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Effect of streptozotocin-induced diabetes mellitus on alveolar bone deposition in the rat

Cited by 55 publications

References 24 publications

Adiponectin and interleukin-6 levels in insulin-treated diabetic rats with experimental periodontitis

Adiponectin and interleukin-6 levels in insulin-treated diabetic rats with experimental periodontitis

Yeni̇ Ti̇p 1 Di̇abetes Melli̇tus Teşhi̇si̇ Konulan Çocuklarin Beslenme Ve Ağiz Hi̇jyeni̇ Eği̇ti̇mi̇ni̇n Değerlendi̇ri̇lmesi̇

Insulin stimulates osteoblast proliferation and differentiation through ERK and PI3K in MG‐63 cells

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