Effect of streptavidin–biotin on endothelial vasoregulation and leukocyte adhesion

Chan, Bernard P.L.; Reichert, William M.; Truskey, George A.

doi:10.1016/j.biomaterials.2003.10.077

Cited by 15 publications

(21 citation statements)

References 53 publications

(79 reference statements)

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“…In particular fluid shear stress resulting from blood flow over the endothelial surface, sensed via a number of mechanosensor mechanisms [84], produces junction [85], cytoskeleton [86] and integrin [87] re-organization which alters ECM organization [88,89], EC morphology [90], and EC gene expression [91][92][93]. This further affects the expression and secretion of proteins associated with EC function, such as regulation of vascular tone [94][95][96], fibrinolysis [97][98][99], surface adhesion [100] and coagulation proteins such as tissue factor and its inhibitor [101,102], thrombomodulin [103,104], prostacyclin [105,106] and von Willebrand factor [107]. Of interest here, is that KLF-2 [108] (Kruppel-like factor), a flow responsive transcription factor is a primary candidate as a central "switch" between quiescent and activated adult EC.…”

Section: Regulation Of Endothelial Cell Phenotypementioning

confidence: 99%

The influence of biomaterials on endothelial cell thrombogenicity

2007

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Driven by tissue engineering and regenerative medicine, endothelial cells are being used in combination with biomaterials in a number of applications for the purpose of improving blood compatibility and host integration. Endothelialized vascular grafts are beginning to be used clinically with some success in some centers, while endothelial seeding is being explored as a means of creating a vasculature within engineered tissues. The underlying assumption of this strategy is that when cultured on artificial biomaterials, a confluent layer of endothelial cells maintain their nonthrombogenic phenotype. In this review the existing knowledge base of endothelial cell thrombogenicity cultured on a number of different biomaterials is summarized. The importance of selecting appropriate endpoint measures that are most reflective of overall surface thrombogenicity is the focus of this review. Endothelial cells inhibit thrombosis through three interconnected regulatory systems (1) the coagulation cascade (2) the cellular components of the blood such as leukocytes and platelets and (3) the complement cascade, and also through effects on fibrinolysis and vascular tone, the latter which influences blood flow. Thus, in order to demonstrate the thromobgenic benefit of seeding a biomaterial with EC, the conditions under which EC surfaces are more likely to exhibit lower thrombogenicity than unseeded biomaterial surfaces need to be consistent with the experimental context. The endpoints selected should be appropriate for the dominant thrombotic process that occurs under the given experimental conditions.

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Section: Regulation Of Endothelial Cell Phenotypementioning

confidence: 99%

The influence of biomaterials on endothelial cell thrombogenicity

2007

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“…At 24-h attachment, EC seeded on ePTFE produced significantly more prostacyclin than EC seeded on glass slide (p Ͻ 0.05), which was measured previously at 0.03 Ϯ 0.002 M/10 6 cells. 23 Cell sodding did not have significant effect on the prostacyclin production at either attachment time point (Table II, …”

Section: Results From In Vitro Experimentsmentioning

confidence: 93%

“…At 24 h of attachment, adherent EC become antithrombotic and release nitric oxide and prostacyclin when shear stress was encountered. 23 The release of these agents may then reduce platelet aggregation and fibrin formation. We found that at 24 h of cell attachment, EC release significantly more prostacyclin than the 1 h cell attachment cases.…”

Section: Discussionmentioning

confidence: 99%

“…20 -22 The use of the dual-ligand system also accelerated the adherent EC to reach the quiescent state that may have additional advantages in reducing graft thrombogenecity. 23 In the presence of physiological shear stress, the production of nitric oxide and prostacyclin were significantly enhanced, and the adhesion of leukocytes onto EC surfaces was inhibited. 23 EC sodding is another approach that accelerates the formation of an EC monolayer on synthetic grafts.…”

Section: Introductionmentioning

confidence: 99%

“…23 In the presence of physiological shear stress, the production of nitric oxide and prostacyclin were significantly enhanced, and the adhesion of leukocytes onto EC surfaces was inhibited. 23 EC sodding is another approach that accelerates the formation of an EC monolayer on synthetic grafts. 25 Intraluminal pressure is applied to force the seeded EC into the interstices of prosthetic grafts.…”

Section: Introductionmentioning

confidence: 99%

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In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts

et al. 2004

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In this study, we examined combinations of three approaches to improve the adhesion of endothelial cells (EC) onto expanded polytetrafluoroethylene (ePTFE) vascular grafts placed at the femoral artery of rats: (1) high-affinity receptor-ligand binding of RGD-streptavidin (SA) and biotin to supplement integrin-mediated EC adhesion; (2) cell sodding to pressurize the seeded EC into the interstices of the ePTFE grafts; and (3) longer postseeding attachment time from 1 to 24 h prior to implantation. An in vitro system, which accounts for cell loss due to both graft handling and shear stress, was designed to optimize conditions for in vivo experiments. Results suggest that longer in vitro attachment time enabled the adherent EC to endure mechanical stresses by forming strong adhesions to the underlying extracellular matrix substrates; cell sodding helped to retain the adherent EC by physically docking the cells against the graft interstices; and the SA-biotin interaction enhanced the early attachment of EC but did not lead to better cell retention or reduced surface coverage of blood clot in the current study. Mechanical manipulation of cells during implantation is a limiting factor in maintaining a confluent EC layer on synthetic vascular grafts.

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Synergistic effect of shear stress and streptavidin‐biotin on the expression of endothelial vasodilator and cytoskeleton genes

Chan

Reichert

Truskey

2004

Biotech & Bioengineering

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Dual ligand treatment of streptavidin(SA)-biotin and fibronectin (Fn) enhances the adhesion of endothelial cells (EC) onto synthetic surfaces and promotes the quiescent phenotype of adherent EC. The current study investigates the effect of the dual ligand on the expression of endothelial genes in static culture and under shear stress (4 h at 10 dynes/cm2). Expression of 23 genes in the classes of signaling, cytoskeleton/ECM, vasoregulation, and shear-responsive were examined. Eight genes (argininosuccinate synthetase, K+ channel, TGFbeta, Mn-SOD, alpha-tubulin, t-PA, COX2, and eNOS) were significantly upregulated by shear stress. Two genes (caveolin-1 and ET-1) were downregulated by shear stress. Three genes (RhoA, elastin, alpha-actinin) were upregulated by the dual ligand treatment in static culture, and four genes (FAK, elastin, COX2, and eNOS) were upregulated when the dual ligand and shear stress were applied simultaneously. Northern blot analyses on FAK, RhoA, elastin, and alpha-actinin revealed similar results. The results suggest (1) the use of SA-biotin to supplement EC adhesion enhances the integrity of the EC cytoskeleton by upregulating the expression of cytoskeleton/ECM genes, and (2) a likely relationship between the expression of cytoskeleton/ECM genes and the downstream events, such as the shear-induced expression of eNOS and COX2 genes. Analyses presented in this study provide insights into the mechanism by which SA-biotin-supplemented EC mediate gene expression.

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Effect of streptavidin–biotin on endothelial vasoregulation and leukocyte adhesion

Cited by 15 publications

References 53 publications

The influence of biomaterials on endothelial cell thrombogenicity

The influence of biomaterials on endothelial cell thrombogenicity

In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts

Synergistic effect of shear stress and streptavidin‐biotin on the expression of endothelial vasodilator and cytoskeleton genes

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