2018
DOI: 10.1016/j.ejpb.2018.02.033
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Effect of storage temperature on the stability of spray dried bacteriophage powders

Abstract: This study aimed to assess the robustness of using a spray drying approach and formulation design in producing inhalable phage powders. Two types of Pseudomonas phages, PEV2 (Podovirus) and PEV40 (Myovirus) in two formulations containing different amounts of trehalose (70% and 60%) and leucine (30% and 40%) were studied. Most of the surface of the produced powders was found to be covered in crystalline leucine. The powders were stored at 4 °C and 20 °C under vacuum. The phage stability and in vitro aerosol per… Show more

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Cited by 61 publications
(47 citation statements)
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References 29 publications
(51 reference statements)
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“…Delivering them as aerosols can achieve direct targeting in the lungs. The feasibility of bioengineering phages into inhalable powders by spray drying has been demonstrated in vitro 1‐6 along with efficacy studies in vivo 7,8 . In the production process, it is essential to preserve phage bioactivity in the dry state by suitable excipients.…”
Section: Introductionmentioning
confidence: 99%
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“…Delivering them as aerosols can achieve direct targeting in the lungs. The feasibility of bioengineering phages into inhalable powders by spray drying has been demonstrated in vitro 1‐6 along with efficacy studies in vivo 7,8 . In the production process, it is essential to preserve phage bioactivity in the dry state by suitable excipients.…”
Section: Introductionmentioning
confidence: 99%
“…In the production process, it is essential to preserve phage bioactivity in the dry state by suitable excipients. Disaccharides such as trehalose, sucrose, and lactose are reported to provide protection to phages during drying and subsequent storage 1‐6 . This concept of sugar‐imparted phage protection was originated from protein stabilization mechanisms 9,10 .…”
Section: Introductionmentioning
confidence: 99%
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“…Given the proteinaceous composition of viruses, shear forces or variations in the temperature and pH to which phages are subjected during handling may impact titers as a result of protein misfolding or denaturation. Their shelf life may vary by bacteriophage species, as a result of phage size and morphology (29,(36)(37)(38)(39). The storage methods utilized in the Republic of Georgia, where phage therapy is used clinically, have included freezedrying, freezing, refrigeration, and short-term ambient storage (Marina Tediashvili, personal communication), though some studies suggest that phase changes, including freezing or drying, may result in drops in titer, at least among certain phages (37).…”
mentioning
confidence: 99%