Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood

Nurhasan, Mulia; Roos, Nanna; Henao, Juan J. Aristizabal; Chamnan, Chhoun; Lauritzen, Lotte

doi:10.1016/j.plefa.2015.02.001

Cited by 9 publications

(5 citation statements)

References 14 publications

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“…Dried blood spot (DBS) sampling is used regularly to screen for congenital diseases such as phenylketonuria, cystic fibrosis, and sickle cell disease. − The convenient collection process has led to DBS sampling being used increasingly in field studies for various blood measurements, , and the use of DBS in lipid research is increasing. , While there is a substantial body of research adapting DBS for fatty acid profiling using gas chromatography, ,,,− we are aware of only two reports of the use of DBS for lipidomic profiling by high-performance liquid chromatography and mass spectrometry. , These previous investigations report only selected complex lipids and other complex lipids known to be in whole blood have been ignored. In fact, lipidomic profiling of whole blood in general is understudied as most of the available literature on lipidomic profiling of human blood examines plasma, − erythrocytes − or leukocytes/platelets. − Whole blood analysis is challenging as the lipidome is a complex mixture of both nonpolar and polar lipids with ranges of abundance and a comprehensive comparison of the lipidome of whole blood and DBS is required.…”

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confidence: 99%

“…Dried blood spot (DBS) sampling is used regularly to screen for congenital diseases such as phenylketonuria, cystic fibrosis, and sickle cell disease. 4−6 The convenient collection process has led to DBS sampling being used increasingly in field studies for various blood measurements, 7,8 and the use of DBS in lipid research is increasing. 9,10 While there is a substantial body of research adapting DBS for fatty acid profiling using gas chromatography, 3,7,8,11−14 we are aware of only two reports of the use of DBS for lipidomic profiling by high-performance liquid chromatography and mass spectrometry.…”

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confidence: 99%

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Tailored Extraction Procedure Is Required To Ensure Recovery of the Main Lipid Classes in Whole Blood When Profiling the Lipidome of Dried Blood Spots

2016

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The use of dried blood spots has increased in research and clinical settings recently, particularly in field studies and screening, but comprehensive acyl-specific lipidomic profiling of dried blood spots has yet to be examined. An untargeted ultrahigh-performance liquid chromatography-tandem mass spectrometry method was adapted for the analysis of lipid extracts from human whole blood samples and dried blood spots collected on chromatography paper. Lipid recoveries were examined after different durations of exposure to extraction solvents (chloroform/methanol), physical disruption (homogenization or sonication) of the paper containing the dried blood spots, and acidification of extraction solvents. We demonstrated that comprehensive untargeted profiles can be obtained from dried blood spot samples that are comparable with whole blood for several species of lipids including phosphatidylcholine, lyso-phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, triacylglycerol, and cholesteryl ester. However, homogenization of the dried blood spots, followed by a 24 h exposure to solvents, and extraction with an acidic buffer (0.2 M NaHPO + 0.1 M hydrochloric acid) was required. Dried blood spots can be used for comprehensive, untargeted lipidomics of the most abundant lipid species in whole blood, but additional sample processing steps are required.

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Tailored Extraction Procedure Is Required To Ensure Recovery of the Main Lipid Classes in Whole Blood When Profiling the Lipidome of Dried Blood Spots

2016

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“…Additionally, work performed in African savanna elephants comparing DBS samples to liquid whole blood, serum, and plasma found minimal differences between the four sample types, suggesting that comparing across sample type is possible ( Wood et al, 2021a ). A study conducted by Nurhasan et al (2015) focused on fatty acid stability of whole blood in humans with varying storage temperatures that included comparing −20 °C to −80 °C storage (9–11 months). The results of this study found that there were more highly unsaturated fatty acids and a lower omega-6 to omega-3 ratio in samples stored at −20 °C ( Nurhasan et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…A study conducted by Nurhasan et al (2015) focused on fatty acid stability of whole blood in humans with varying storage temperatures that included comparing −20 °C to −80 °C storage (9–11 months). The results of this study found that there were more highly unsaturated fatty acids and a lower omega-6 to omega-3 ratio in samples stored at −20 °C ( Nurhasan et al, 2015 ). While valuable, other research suggests that −75 °C or below is better for PUFA and total lipid extracts in serum, plasma, whole blood, and chromatography paper as it stops nearly all peroxidation ( Metherel & Stark, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Assessment of the effects of storage temperature on fatty acid analysis using dried blood spot cards from managed southern white rhinoceroses (Ceratotherium simum simum): implications for field collection and nutritional care

Wood

Minter

Bibus

et al. 2022

PeerJ

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Background Southern white rhinoceroses (Ceratotherium simum simum) are an endangered species in decline due to poaching and negative habitat changes. Conservation of the species has become increasingly important and a focus on better human management has become prevalent. One area of management that impacts southern white rhinoceroses is nutritional health monitoring, which is often conducted through blood analysis. Blood analysis conducted during field research can be difficult due to temperature, distance, and limited technological resources, so new methods of fast, and relatively stable blood collection are being pursued. One method that has been used in humans for many years is beginning to make its way into wildlife studies: the use of dried blood spot (DBS) cards. These cards are used as a tool to store single drops of whole blood on specialized filter paper and, once dried, can be used for nutritional biomarker analysis. An area of interest for southern white rhinoceroses and nutrition is monitoring fatty acid percentages for cardiovascular, immune, and reproductive health. The time and temperature limitations for storing blood fractions or liquid whole blood when analyzing fatty acids have been investigated, but few studies have performed storage studies on DBS cards colder than −20 °C or in non-human species. Methods In order to better understand the limitations of DBS cards and the impact of temperature on fatty acid DBS samples in long-term storage, triplicate samples from seven adult southern white rhinoceroses at the North Carolina Zoo were collected and subjected to three storage treatments (immediate, room temperature (23 °C), or frozen (−80 °C) for 1 year). Results Stearidonic (18:4w3) (Δ 0.3%), arachdic (20:0) (Δ 0.1%), eicosatetraenoic (20:4w3) (Δ 0.2%), and erucic acid (22:1w9) (Δ 0.1%) were in higher concentration in frozen than initial. Fatty acids in higher concentrations in the initial samples than frozen were myristic (14:0) (Δ 0.2%), mead (20:3w9) (Δ 0.1%), docosatetraenoic (22:4w6) (Δ 0.2%), nervonic (24:1) (Δ 0.1%), and total highly unsaturated fatty acids (HUFAs) (Δ 0.7%). Stearic (18:0) (Δ 2.2%), stearidonic (18:4w3) (Δ 0.3%), arachdic (20:0) (Δ 0.2%), paullinic (20:1w7) (Δ 0.4%), eicosatetraenoic (20:4w3) (Δ 0.1%), eicosapentaenoic (20:5w3) (Δ 0.1%), docosatetraenoic (22:4w6) (Δ 0.2%), nervonic acid (24:1) (Δ 0.2%), monoenes (Δ 1.9%), and total saturates (Δ 3.6%) had higher concentrations in room temperature than initial. Linoleic (18:2w6) (Δ 4.9%), mead acid (20:3w9) (Δ 0.1%), total polyunsaturated fatty acids (5.3%), and total omega-6 fatty acids (Δ 4.8%) had higher concentrations in initial compared to room temperature. Arachidonic (20:4w6) (Δ 0.4%) and omega-3 docosapentaenoic acid (22:5w3) (Δ 0.1%), had higher concentrations in frozen than in room temperature. Discussion The frozen samples had the fewest statistical differences compared to room temperature samples and essential omega-3 and -6 fatty acids were stable with freezing up to 1 year. While more research is still warranted, current results suggest that DBS samples are best utilized when immediate analysis or −80 °C storage is available.

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“…Storage time in the field prior to arrival in Canada ranged from 4-6 months and total storage period before analysis varied from 9 to 11 months. An assessment of LCPUFA stability during storage and more details about the blood sampling procedures are available in [22].…”

Section: Blood Samplingmentioning

confidence: 99%

Effect of complementary food with small amounts of freshwater fish on whole blood n-3 fatty acids in Cambodian infants age 6–15 months

Nurhasan

Roos

Skau

et al. 2018

Prostaglandins, Leukotrienes and Essential Fatty Acids

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The impact of freshwater fish consumption on the status of long-chain n-3 fatty acids (n-3 LCPUFA) in infants in landlocked, low-income populations is unknown. We used secondary data from a randomized, single-blinded, controlled trial to evaluate the impact of daily consumption of complementary food products with small amounts of freshwater fish on whole blood n-3 LCPUFA in Cambodian infants. Infants (n = 419), received daily, one of four food products for 9 months. Two products contained freshwater fish: WinFood (10% fish by dry weight) and WinFood-L (12% fish by dry weight), while two products were non-fish-based: corn-soy blends (CSB+ and CSB++). Whole blood fatty acids and breastfeeding status were assessed at baseline and endline of the intervention. The WinFood products contributed to an estimated maximum intake of 86.5 mg/day n-3 LCPUFA. There was no difference in whole blood n-3 LCPUFA among the four intervention groups or between the fish-based and the non-fish-based groups (p ≥ 0.142). At endline, 71% of the children were still breastfed. Interaction analyses indicated a lower ratio of n-6/n-3 PUFA in non-breastfed infants in the WinFood groups compared to the CSB groups (p= 0.026). Thus, a high intake of n-3 LCPUFA from breastmilk may have blurred a potential impact of small amounts of freshwater fish effect on n-3 LCPUFA status in Cambodian infants.

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Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood

Cited by 9 publications

References 14 publications

Tailored Extraction Procedure Is Required To Ensure Recovery of the Main Lipid Classes in Whole Blood When Profiling the Lipidome of Dried Blood Spots

Tailored Extraction Procedure Is Required To Ensure Recovery of the Main Lipid Classes in Whole Blood When Profiling the Lipidome of Dried Blood Spots

Assessment of the effects of storage temperature on fatty acid analysis using dried blood spot cards from managed southern white rhinoceroses (Ceratotherium simum simum): implications for field collection and nutritional care

Effect of complementary food with small amounts of freshwater fish on whole blood n-3 fatty acids in Cambodian infants age 6–15 months

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