Ribeye, top round, and eye of round steaks were treated for 30 min with 100% carbon monoxide (CO), vacuum packaged, and held in refrigerated storage. Instrumental color determinations (L*, a*, and b* values) were made on uncooked ribeye and round steaks every 7days. Aerobic plate counts (APC), psychrotrophs, and lactic acid bacteria (LAB) were determined on eye of round samples after 1, 4, and 8 weeks. Initially, CO-treated steaks were more red (higher a* values) than untreated steaks. The a* values of treated steaks decreased during storage; at 6 weeks no differences existed due to CO treatment. CO-treated steaks were more yellow (higher b* values) at all storage times than untreated steaks. APC of CO-treated samples were 1 log cycle lower than control after 8 weeks in storage; LAB counts were nearly 1 log cycle lower for CO-treated samples after 8 weeks. Psychrotrophic counts were similar for control and CO-treated samples during the first 4 weeks of refrigerated storage but were nearly 2 log cycles lower for CO-treated samples after 8 weeks. These data suggest that CO-treated steaks are more red and that they have extended shelf-lives compared with untreated, vacuum-packaged steaks.