Effect of Storage on the Virulence of Gypsy Moth Nucleopolyhedrosis Inclusion Bodies12

Lewis, Franklin B.; Rollinson, W. D.

doi:10.1093/jee/71.5.719

Cited by 16 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A similar loss of efficacy in the same virus over a 5 year storage period was found by Martignoni (1978). Others have also reported loss of potency of insect viruses under various storage conditions over time: including the eastern hemlock looper virus (Cunningham, 1970), for the gypsy moth virus (Lewis and Rollinson, 1978), and the European spruce sawfly virus (Neilson and Elgee, 1960).…”

Section: Discussionmentioning

confidence: 99%

Effects of long-term storage on the stability of OpMNPV DNA contained in TM Biocontrol-1

Reed

Otvos

Reardon

et al. 2003

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Effects of long-term storage on the stability of OpMNPV DNA contained in TM Biocontrol-1

Reed

Otvos

Reardon

et al. 2003

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

“…Bergold (1958, cited in Lewis andRollinson 1978) reported that polyhedra of Bombyx mori (L.) lost little or no activity after 9 yr. In contrast to these reports, Cunningham (1970) demonstrated a loss of insecticidal activity for a baculovirus of the hemlock looper, Lambdina fiscellaria fiscellaria (Guenee), stored for 6 yr as an aqueous suspension under refrigerated conditions, and suggested that freeze-drying may be better for storing virus.…”

Section: Discussionmentioning

confidence: 99%

“…Pest control efÞcacy in the Þeld is often compromised because of the rapid loss of insecticidal activity resulting from exposure to sunlight Granados 1991, Huber andLudcke 1996). Finally, baculoviruses have been shown to lose activity when stored as a dry preparation for extended periods of time (Lewis andRollinson 1978, Kaupp andEbling 1993). All of these characteristics combine to raise the cost to beneÞt ratio for baculovirus insecticides and thus baculoviruses account for Ͻ0.1% of operational pest control in highly industrialized nations (Federici 1999).…”

mentioning

confidence: 99%

Field Activity and Storage Stability of <I>Anagrapha falcifera</I> Nucleopolyhedrovirus (<I>Af</I>MNPV) in Spray-Dried Lignin-Based Formulations

Behle

Tamez‐Guerra

McGuire³

2003

View full text Add to dashboard Cite

A multiple-embedded nucleopolyhedrovirus isolated from Anagrapha falcifera (Kirby) (AfMNPV) has potential to be developed into a microbial bioinsecticide because the host range includes several economic pests. We tested spray-dried AfMNPV formulations after storage for insecticidal activity based on bioassays with neonate Trichoplusia ni (Hübner). Eight experimental lignin-based spray-dried formulations, a glycerin-based formulation, and an unformulated sample were made with virus stock from three commercial production lots. Samples of these formulations were stored at 30 degrees C in individually sealed sample containers for destructive sampling after 1, 3, and 6 mo whereas the remaining product was stored in glass jars under refrigeration for up to 30 mo. Spray drying did not significantly reduce the initial LC50s of AfMNPV in experimental formulations compared with unformulated virus that was not spray dried. Refrigerated storage for 6 mo did not significantly lower virus activity of formulated samples compared with the unformulated AfMNPV stored frozen, while samples stored for 30 mo had higher LC50 values determined by both droplet and leaf feeding assays. When stored at 30 degrees C, most formulations (22 of 24) maintained insecticidal activity for 3 mo, but most (21 of 24) lost significant activity after 6 mo of storage. The glycerin-based formulation also lost activity within 6 mo of storage at 30 degrees C when compared with frozen unformulated virus, but did not lose activity when stored refrigerated for up to 30 mo. These formulations were evaluated after 7 mo at 4 degrees C for residual insecticidal activity when applied to field grown cabbage. Insecticidal activity was determined against T. ni neonates for treated leaf samples collected at 3, 7, 27, and 51 h after application of 2.5 x 10(12) obs/ha. Field tests showed no differences in activity among samples of stored formulations and one freshly made formulation. Spray-dried formulations had significantly higher insecticidal activity (67.5% mortality) compared with the unformulated treatment (30% mortality) sampled 3 h after application. At 3, 7, and 27 h after application, the spray-dried formulations had higher residual activity (67%, 59%, and 42% mortality, respectively), compared with the commercial glycerin-based formulation (61%, 38%, and 23% mortality, respectively). These experiments demonstrated that AfMNPV in lignin-based spray-dried formulations had a shelf-life of up to 3 mo at 30 degrees C and up to 30 mo at 4 degrees C, and with longer residual insecticidal activity in the field compared with unformulated or a glycerin formulation.

show abstract

“…Control larvae were fed untreated diet. Other groups of 50 larvae were fed diet containing known concentrations (LC z 5-LC75) of gypsy moth NPV (Hamden Standard K) (LEwiS & ROLLINSON, 1978). All larvae were reared for 16 days at 23 _+ 2~ and a 16-h photoperiod.…”

Section: Bioassaysmentioning

confidence: 99%

“…The number of polyhedral inclusion bodies (PIB)/g of tissue that this activity represented was estimated by extrapolation from dose-mortality curves generated from Hamden Strain K NPV bioassay data (LEWIS & ROLLINSON, 1978).…”

Section: Bioassaysmentioning

confidence: 99%

Natural occurrence of the nucleopolyhedrosis virus of the gypsy moth,Lymantria dispar [Lep.: Lymantriidae] in wild birds and mammals

1980

View full text Add to dashboard Cite

Effect of Storage on the Virulence of Gypsy Moth Nucleopolyhedrosis Inclusion Bodies12

Cited by 16 publications

References 0 publications

Effects of long-term storage on the stability of OpMNPV DNA contained in TM Biocontrol-1

Effects of long-term storage on the stability of OpMNPV DNA contained in TM Biocontrol-1

Field Activity and Storage Stability of <I>Anagrapha falcifera</I> Nucleopolyhedrovirus (<I>Af</I>MNPV) in Spray-Dried Lignin-Based Formulations

Natural occurrence of the nucleopolyhedrosis virus of the gypsy moth,Lymantria dispar [Lep.: Lymantriidae] in wild birds and mammals

Contact Info

Product

Resources

About