Effect of Storage of Rabbit Spermatozoa at?79°C on Their Subsequent Transport and Fertility in the Rabbit Doe

Murdoch, BE; O’Shea, Tim

doi:10.1071/bi9730645

Cited by 8 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although no reduction in sperm motility was found, fewer spermatozoa were seen in the mucin coat and zona pellucida of embryos recovered from does inseminated with spermatozoa frozen for 5 hr. Murdoch & O'Shea (1973) previously reported that fewer supplementary spermatozoa were associated with ova recovered from does inseminated with frozen spermatozoa than those from does inseminated with fresh semen. Freezing and thawing of mammalian cells have been reported to damage mitochondria, ribosomes, lysosomes, and the endoplasmic reticulum (Tappel, 1966;Quinn & White, 1966;Quinn, White & Cleland, 1969), The loss and return of the fertilizing ability of frozen spermatozoa might have been due to a time-related repair of damage caused by the freezing process and/or ice crystal formation changes with time causing less cellular damage during thawing (Rapatz & Luyet, 1961).…”

Section: Effects On Fertilizationmentioning

confidence: 96%

Embryonic development in rabbits after insemination with spermatozoa stored at 37, 5 or -196 C for various periods

Maurer¹,

Stranzinger²,

Paufler³

1976

Reproduction

View full text Add to dashboard Cite

Rabbit spermatozoa stored at 37 degrees C for 5 hr, 5 degrees C for 5 hr or 5 days, and-196 degrees C for 5 hr, 5 days, or 1 year were used to artificially inseminate does induced to ovulate normal or excess numbers of eggs. Fertility and subsequent embryonic development were examined. Frozen spermatozoa stored for 5 hr fertilized fewer oocytes than those stored for 5 days or longer. Fewer embryos were recovered on Day 6 than on Day 2. Blastocysts were smaller when spermatozoa were stored for 5 days, were frozen or were placed in superovulating does, but no statistically significant differences in the % of fetal survival were found in the groups of normally ovulating does. Embryonic wastage associated with storage of spermatozoa resulted from a reduced fertilization rate and/or increased embryonic mortality before implantation.

show abstract

Section: Effects On Fertilizationmentioning

confidence: 96%

Embryonic development in rabbits after insemination with spermatozoa stored at 37, 5 or -196 C for various periods

Maurer¹,

Stranzinger²,

Paufler³

1976

Reproduction

View full text Add to dashboard Cite

show abstract

“…IV), which was in agreement with the results reported by Maurer et al [9], who observed a 45% cleavage rate (from total recovery) when inseminating with frozen sperm. The low number of total embryos recovered could indicate a problem in the transport of sperm (very few sperm are able to reach the oviducts to fertilise oocytes, although Murdoch and O'Shea, [12], observed fewer sperm in the oviducts of females inseminated with frozen sperm, but the differences between fresh and frozen sperm were not significant) since other authors have reported fewer accessory sperm in oocytes from females inseminated with frozen sperm [9,12,14]. This could also indicate a very low viability of the spermatozoa (they could reach the oviducts, but they would remain alive for a very short time) or any other problems which could 195 …”

Section: Discussionmentioning

confidence: 99%

Effect of cooling and freezing, the two first steps of a freezing protocol, on the fertilizing ability of the rabbit sperm

Mocé

Vicente

2002

Reprod. Nutr. Dev.

View full text Add to dashboard Cite

-The effect of different phases of a freezing protocol on the fertilising ability of rabbit spermatozoa was evaluated. An extender containing 1.75 M DMSO and 0.05 M sucrose (final concentration) was used to freeze rabbit sperm. In the first experiment, the results obtained with fresh and cooled (5 ºC for 45 min) sperm were compared; no differences were observed between fresh and cooled semen for any of the parameters studied: fertility rate (78% vs. 91% for fresh and cooled sperm, respectively), and normal embryos two days after insemination (6.8 vs. 8.5 normal embryos for fresh and cooled sperm). In the second experiment, the results obtained with fresh semen and sperm which had passed the first two steps of a freezing protocol (5 ºC for 45 min and -30°C for 30 min, and thawed at 50 °C for 15 s) were compared; the differences between them were obtained for fertility rate (94% vs. 61% for fresh and frozen sperm, respectively) and normal embryos two days after insemination (7.8 vs. 3.8 fresh and frozen sperm). These observations indicated that the differences in the results obtained with fresh and cryopreserved sperm were produced during the second step of the freezing protocol, and that apparently no toxic effect of DMSO was produced.embryo / fertility / freezing / rabbit / sperm Reprod. Nutr. Dev. 42 (2002) [189][190][191][192][193][194][195][196] 189

show abstract

“…Early attempts to achieve fertilization with rabbit semen frozen and thawed in the presence of glycerol were relatively unsuccessful, but better results were achieved using ethylene glycol (Fox & Burdick, 1963) or dimethylsulphoxide (Sawada & Chang, 1964) as cryoprotective agents. High kindling rates with frozen rabbit semen have been documented and observations have been made on several factors relating to rabbit semen freezing, such as sperm numbers for inseminations (Andrieu & Courot, 1976), acrosome morphology (Weitze, Hellemann & Krause, 1976;Hellemann, 1977), storage periods (O'Shea & Wales, 1969;Maurer, Stranzinger & Paufler, 1976), sperm transport in the female reproductive tract (Murdoch & O'Shea, 1973) and freezing procedures (Stranzinger, Maurer & Paufler, 1971;Weitz et al, 1976). However, no cryoprotective agents other than glycerol, dimethylsulphoxide and ethylene glycol have been examined for the low temperature preservation of rabbit semen.…”

Section: Introductionmentioning

confidence: 96%

Cryoprotective effects of some amides on rabbit spermatozoa

Hanada¹,

Nagase²

1980

Reproduction

View full text Add to dashboard Cite

Semen was diluted 1:9 with egg yolk-citrate medium containing 0.31--3.1 M (final concentration) formamide, butyramide, acetamide, propionamide, dimethylformamide, lactamide, malomide, ethylene glycol, trimethylene glycol, dimethylsulphoxide (DMSO) or glycerol. After 30 min incubation at 20 degrees C, sperm motility was superior in hypertonic solutions of acetamide, lactamide, dimethylsulphoxide, trimethylene glycol and ethylene glycol. Some of these compounds were added to semen diluted 1:2 in an isotonic egg-yolk-glucose-lactose-raffinose solution and frozen by the pellet method. Relatively good survival of motility was obtained in 1.0 M-DMSO, -lactamide or -acetamide. Dimethylformamide (0.5 M), ethylene glycol (0.5--1.5 M), trimethylene glycol (1.5 M) and propionamide (0.75 M) also gave some protection. Insemination of does with semen frozen and thawed with 1.0 M-DMSO, -lactamide or acetamide gave fertilization rates of 68--88%, and 84% (38/45) of does gave birth to an average of 5.3 young.

show abstract

Effect of Storage of Rabbit Spermatozoa at?79°C on Their Subsequent Transport and Fertility in the Rabbit Doe

Cited by 8 publications

References 2 publications

Embryonic development in rabbits after insemination with spermatozoa stored at 37, 5 or -196 C for various periods

Embryonic development in rabbits after insemination with spermatozoa stored at 37, 5 or -196 C for various periods

Effect of cooling and freezing, the two first steps of a freezing protocol, on the fertilizing ability of the rabbit sperm

Cryoprotective effects of some amides on rabbit spermatozoa

Contact Info

Product

Resources

About