Effect of storage conditions on the shelf-life extension of fungus-colonized substrates based on Metarhizium anisopliae using modified atmosphere packaging

Jeong, Seul-Gi; Kim, Ho Myeong; Kim, Junheon; Park, Hae Woong

doi:10.1038/s41598-021-04232-5

Cited by 11 publications

(7 citation statements)

References 31 publications

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“…This can be achieved through the addition of protectants and stabilizers during the formulation process, through the use of protective gases during storage and through the use of certain materials to manufacture the storage vessels. [22][23][24] One of us has had good experiences using High Density Polyethylene bottles overlaying the product with pure nitrogen (P.L., unpublished). However, even during the cultivation of the fungus to produce the conidia, the subsequent storage stability can be influenced.…”

Section: Discussionmentioning

confidence: 99%

A microbial bioherbicide for Striga hermonthica control: production, development, and effectiveness of a seed coating agent

Lüth¹,

Nzioki

Baker³

et al. 2023

Pest Management Science

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Witchweed (Striga hermonthica), also called striga, is a parasitic weed that causes high yield losses in maize on more than 200 000 ha in Kenya alone. A new commercial, biological herbicide developed in Kenya is able to control striga effectively. The product was approved for use by the Pest Control Products Board in Kenya in September, 2021. It is self‐produced in villages using a secondary inoculum provided by a commercial company. The formulated product has some disadvantages, which are a complicated production process, a very short shelf life and high application rate. Additionally, the product has to be applied manually and therefore can only be used in manual production, leaving out the opportunity for farmers using mechanization. For this reason, efforts have been made to formulate the active ingredient Fusarium oxysporum f. sp. strigae strain DSM 33471, as a powder and to use it as a seed coating agent. This article deals with the production of the Fusarium spore powder, its properties, its application to the seed, and its herbicidal effect demonstrated in the first two field trials. The F. oxysporum strain was originally isolated from a wilting striga plant in Kenya. The strain was virulence enhanced to over produce the amino acids leucine, methionine and tyrosine. These amino acids are responsible for a second mode of action apart from the wilting causing effect of the fungus on striga. Whereas leucine and tyrosine have a herbicidal effect, ethylene from methionine triggers the germination of striga seeds in the soil. Additionally, the strain has been improved to be resistant to the fungicide captan, which is commonly used to treat maize seed in Kenya. Seed coating tests conducted on 25 striga‐infested small holder farms spread out in six counties of western Kenya reported yield increases of up to 88%. A second trial carried out by the Kenyan Agricultural and Livestock Research Organization showed a 93% reduction of emerged striga plants. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

A microbial bioherbicide for Striga hermonthica control: production, development, and effectiveness of a seed coating agent

Lüth¹,

Nzioki

Baker³

et al. 2023

Pest Management Science

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“…In future studies, strategies for extending the shelf life of conidia at room temperature need to be addressed. These strategies may include reducing and preserving the moisture content of conidia down to 4-5% at large-scale (Moore et al, 1996), modifying the atmosphere packaging (CO2 ≈ 90%) to reduce the metabolism of conidia (Jeong et al, 2022), and exploring the formulation of the conidia with biodegradable polymers (De la Cruz Quiroz et al, 2019). For now, our results suggest that C. javanica conidia should be distributed and used in the medium term to maintain their e cacy in eld conditions but to achieve it the supply chain of the mycoinsecticide must be optimized.…”

Section: Discussionmentioning

confidence: 99%

Palm kernel meal improves the production and conidia quality of Cordyceps javanica in conventional solid-state fermentation

Ordaz-Hernández,

Montesinos-Matías,

Mellín-Rosas

et al. 2023

Preprint

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Palm kernel meal was incorporated into rice, and its effect on conidiation by Cordyceps javanica under solid-state fermentation was determined. The addition of 5% palm kernel meal increased the conidial yield by up to 40%, without compromising conidia viability and hydrophobicity. In addition, conidia caused higher levels of mortality by mycosis against Diaphorina citriadults (90%), relative to conidia harvested from rice (52%). The conidia recovered from rice/palm kernel meal mixtures also retained viability greater than 90% after storage for 10 months at 4 °C, while the conidia produced on rice reached 80%. Thus, conidia produced in the presence of palm kernel meal can be consumed immediately or in the medium term. Some advantages of palm kernel meal as a co-substrate are also analyzed in the conventional production system of C. javanica. These results are attractive for improving the mycoinsecticide production process, with excellent cost-benefit and minimal changes in infrastructure and process.

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“…Furthermore, a study evaluated an alternative modified atmosphere packaging (MAP) system to preserve M. anisopliae-inoculated millet grains, tested different packaging materials (e.g., polypropylene, polyethylene terephthalate, ethylene-vinyl alcohol), gas compositions, and storage temperatures (4-25 °C). The ethylene-vinyl alcohol film displayed satisfactory results by preserving the dry conidium viability at 80.5% with 7.4% moisture for 28 days at 4 °C due to the package modified atmosphere (30% CO 2 + 70% N 2 ) (Jeong et al, 2022). In this work, the selected conidium:cornstarch tablet type formulation (30:70%) remained viable at marketdesired levels after 180-days period, without the need of refrigeration when preserved in polyethylene pot with desiccator (polymerized silica) and airtight closure (PSP pot).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have focused their efforts on evaluating the packaging compositions to prolong the shelf life of fungal biopesticides. Furthermore, the excipient formulation and packaging type combinations are important factors for efficient product preservation (de la Cruz Quiroz et al, 2019;Biryol et al, 2022;Jeong et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Technology of a novel conidia-tablet formulation and packaging type to increase Beauveria bassiana (Hypocreales: Ophiocordycipitaceae) shelf life at room temperature

Almeida¹,

Varize²,

Santos³

et al. 2023

Biotechnol. Agron. Soc. Environ.

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Description of the subject. This study describes development of mycoinsecticide formulations containing dry conidia of the entomopathogenic fungus Beauveria bassiana combined with various inert excipients in tablet form to facilitate conidia dispersion in water and to maintain viability during storage. Objectives. This study aimed to evaluate the tablet formation of B. bassiana conidia associated with different inert excipient under direct compression in a hydraulic press. Method. Evaluation and validation of the mechanical and storage properties of conidial tablets in different types of packaging. Results. The tablet formulation containing 30% conidia (active ingredient) and 70% cornstarch (inert excipient) displayed satisfactory results regarding mechanical resistance and dispersion in water. This tablet exhibited hardness and friability of 173.94 N and 0.34%, respectively. The tablet disintegration rate in water occurred in 2 min, presenting a rapid dispersion of 109 conidia∙ml-1. Tablet stored in polyethylene pot with polymerized silica exhibited viability ≥ 80% over 180 days storage (25-28 °C). Conclusions. The formulation-packaging binomial is promising, considering the physical-chemical tablet parameters and the viability data. The conidia:cornstarch tablet type formulation provides a relevant cost-benefit ratio of a simple production process of a bioinsecticide, besides the use of low-cost adjuvant and packaging (polyethylene pot). Considering that most biopesticide products require storage under refrigeration, this work becomes important since the formulation remained viable for 180-days storage under room temperature conditions.

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Effect of storage conditions on the shelf-life extension of fungus-colonized substrates based on Metarhizium anisopliae using modified atmosphere packaging

Cited by 11 publications

References 31 publications

A microbial bioherbicide for Striga hermonthica control: production, development, and effectiveness of a seed coating agent

A microbial bioherbicide for Striga hermonthica control: production, development, and effectiveness of a seed coating agent

Palm kernel meal improves the production and conidia quality of Cordyceps javanica in conventional solid-state fermentation

Technology of a novel conidia-tablet formulation and packaging type to increase Beauveria bassiana (Hypocreales: Ophiocordycipitaceae) shelf life at room temperature

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