Effect of stirrer speed and buffering agents on the production of glucose oxidase and catalase by Penicillium variabile (P16) in benchtop bioreactor

Petruccioli, Maurizio; Fenice, Massimiliano; Piccioni, Paola; Federici, Federico

doi:10.1016/0141-0229(94)00030-1

Cited by 49 publications

(12 citation statements)

References 11 publications

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“…2) on petri plates containing 2-dDG were used for further work on optimization and enzyme production. The frequency of positive mutations and number of viable colonies decreases with an increase in mutagen dose rate (Petruccioli et al 1995). Similarly, in the present study, as the time of exposure increased, the survival of the fungus decreased, with a decrease in viable colonies.…”

Section: Mutagenesis By Ems Treatmentsupporting

confidence: 50%

Hyperproduction of Manganese Peroxidase through Chemical Mutagenesis of Trametes versicolor IBL-04 and Optimization of Process Parameters

Ramzan¹,

Asgher²,

Sheikh³

et al. 2013

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This is the first report on chemical mutagenesis of Trametes versicolor IBL-04 to develop a hyper-producing mutant for overproduction of manganese peroxidase (MnP) using sugarcane bagasse as a substrate. A freshly prepared inoculum of indigenously isolated T. versicolor IBL-04 was treated with 100 µg mL -1 (v/v) ethyl methane sulfonate (EMS) and ethidium bromide (EB) separately for different time periods. The selected mutants and parent strain were cultured in solid-state fermentation (SSF) conditions to select the hyper-producing mutants. After selection of hyper-producing EMS-and EB-treated mutants, the fermentation parameters, including substrate type, incubation time, initial pH of the medium, temperature, moisture level, and carbon-to-nitrogen ratio (C:N), were optimized by adopting the Classical Optimization Strategy. T. versicolor IBL-04 treated for 90 min with EMS (EMS-90 mutant) gave maximum MnP production (935 U mL -1 ) after 8 days of fermentation. Supplementation with carbon and nitrogen sources significantly enhanced mutant growth, and under optimum conditions, the maximum MnP production by the mutant strain increased to 3045 U mL -1. The results indicated that the random chemical mutagenesis significantly enhanced the MnP production. The increased production of MnP by the EMS-90 mutant strain suggest its potential for commercial-scale enzyme production and biotechnological applications.

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Section: Mutagenesis By Ems Treatmentsupporting

confidence: 50%

Hyperproduction of Manganese Peroxidase through Chemical Mutagenesis of Trametes versicolor IBL-04 and Optimization of Process Parameters

Ramzan¹,

Asgher²,

Sheikh³

et al. 2013

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“…1990 ). On the other hand, in the case of Penicillium variabile , a low oxygen saturation (between 1 and 5%) did not appear to have a negative effect on the enzyme production ( Petruccioli et al . 1995 ).…”

Section: Resultsmentioning

confidence: 99%

Production of catalase and glucose oxidase by Aspergillus niger using unconventional oxygenation of culture

Fiedurek

Gromada

2000

J Appl Microbiol

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A novel method for increasing dissolved oxygen concentration in culture media has been developed. It involves adding hydrogen peroxide (H 2 O 2 ) to the medium which is then decomposed to oxygen and water by catalase. Some factors affecting oxygenation of culture were investigated. Maximal oxygen concentration occurred in 50 ml of the medium containing 0Á2 g wet mycelium and 0Á2% glucose at pH 5Á0. A new apparatus for automated addition of H 2 O 2 to the bioreactor to keep the dissolved oxygen concentration constant over the range 1±100% 2% was tested. A signi®cant increase (over sixfold) of intracellular catalase activity was obtained while the dissolved oxygen concentration remained stable (30% 2%).

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“…Although many microorganisms including Saccharomyces cerevisiae CFR-201 (Venkateshwaran et al 1999), Penicillium variable (P16) (Petruccioli et al 1995), Penicilli minioluteum 40 (Kurakov et al 2001) and Alternaria alternata (Caridis et al 1991) have been reported as sources of catalase, their catalase yields usually did not exceed 1,500 U/ml. The fermentation time of 12 h was much shorter than other reports, such as 7 days in Alternaria alternata (Caridis et al 1991), 72 h in Penicillium variabile (P16) (Petruccioli et al 1995), and 72 h in Saccharomyces cerevisiae (Venkateshwaran et al 1999). A short fermentation time and relatively high catalase production can make Serratia marcescens SYBC-01 as a potential catalase producer.…”

Section: The Optimum Fermentation Conditionsmentioning

confidence: 97%

Optimization of catalase production and purification and characterization of a novel cold-adapted Cat-2 from mesophilic bacterium Serratia marcescens SYBC-01

et al. 2010

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Maximum catalase production by mesophilic bacterium Serratia marcescens SYBC-01 was obtained by an optimization of culture medium and conditions. A novel cold-adapted catalase from the strain was purified and characterized. The Cat-2 without peroxidase activity was a homodimer with a molecular mass of 154 kDa, consisting of two identical subunits of about 70 kDa. Its apparent K m and V max value were 29.7 mM and 80,925 U/ mg of protein, respectively. The Cat-2 exhibited maximal activity at pH 7.0, being relatively stable in alkaline conditions. The enzyme was most active at approximately 20°C and had 73.8% activity at 0°C. After incubation at 60°C for 60 min, the enzyme still maintained 75% of its initial activity. The Cat-2 displayed relatively higher thermostability compared to that of other cold-adapted and some mesophilic catalases.

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Effect of stirrer speed and buffering agents on the production of glucose oxidase and catalase by Penicillium variabile (P16) in benchtop bioreactor

Cited by 49 publications

References 11 publications

Hyperproduction of Manganese Peroxidase through Chemical Mutagenesis of Trametes versicolor IBL-04 and Optimization of Process Parameters

Hyperproduction of Manganese Peroxidase through Chemical Mutagenesis of Trametes versicolor IBL-04 and Optimization of Process Parameters

Production of catalase and glucose oxidase by Aspergillus niger using unconventional oxygenation of culture

Optimization of catalase production and purification and characterization of a novel cold-adapted Cat-2 from mesophilic bacterium Serratia marcescens SYBC-01

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