Effect of sterol carrier protein-2 gene ablation on HDL-mediated cholesterol efflux from cultured primary mouse hepatocytes

Storey, Stephen M.; Atshaves, Barbara P.; McIntosh, Avery L.; Landrock, Kerstin K.; Martin, Gregory G.; Huang, Huan; Payne, H. Ross; Johnson, Jeffery D.; Macfarlane, Ronald D.; Kier, Ann B.; Schroeder, Friedhelm

doi:10.1152/ajpgi.00446.2009

Cited by 33 publications

(60 citation statements)

References 43 publications

(70 reference statements)

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“…The overexpression of SCP-2 may finally inhibit HDL-mediated cholesterol efflux. SCP-2 plays a significant role in HDL-mediated cholesterol efflux by regulating the sizes of the rapid vs slow cholesterol efflux pools [75,76] .…”

Section: Scp-2mentioning

confidence: 99%

A novel model of cholesterol efflux from lipid-loaded cells

et al. 2010

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Cholesterol efflux from lipid-loaded cells is a key athero-protective event that counteracts cholesterol uptake. The imbalance between cholesterol efflux and uptake determines the prevention or development of atherosclerosis. Many proteins and factors participate in the cholesterol efflux event. However, there are currently no systematic models of reverse cholesterol transport (RCT) that include most RCT-related factors and events. On the basis of recent research findings from other and our laboratories, we propose a novel model of one center and four systems with coupling transportation and networking regulation. This model represents a common way of cholesterol efflux; however, the systems in the model consist of different proteins/factors in different cells. In this review, we evaluate the novel model in vascular smooth muscle cells (VSMCs) and macrophages, which are the most important original cells of foam cells. This novel model consists of 1) a caveolae transport center, 2) an intracellular trafficking system of the caveolin-1 complex, 3) a transmembrane transport system of the ABC-A1 complex, 4) a transmembrane transport system of the SR-B1 complex, and 5) an extracelluar trafficking system of HDL/Apo-A1. In brief, the caveolin-1 system transports cholesterol from intracellular compartments to caveolae. Subsequently, both ABC-A1 and SR-B1 complex systems transfer cholesterol from caveolae to extracellular HDL/Apo-A1. The four systems are linked by a regulatory network. This model provides a simple and concise way to understand the dynamic process of atherosclerosis.

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Section: Scp-2mentioning

confidence: 99%

A novel model of cholesterol efflux from lipid-loaded cells

et al. 2010

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“…A role of L-FABP in the attenuation of hepatic steatosis has also been demonstrated (24). SCP-2 expression has also been indicated to play a significant role in high density lipoprotein-mediated cholesterol efflux by regulating the size of rapid versus slow cholesterol efflux pools and/or eliciting the concomitant upregulation of L-FABP in cultured primary hepatocytes (25).…”

Section: Discussionmentioning

confidence: 94%

Changes in FABP1 and gastrin receptor expression in the testes of rats that have undergone electrical injury

Huang

Liu

2015

Experimental and Therapeutic Medicine

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Abstract. Testicular trauma may occur due to accidental electrical injury. The aim of this study was to investigate alterations in the levels of fatty acid-binding protein 1 (FABP1) and gastrin receptor (gastrin R) in the testes following electrical injury. Sprague-Dawley rats were divided into control, fatal electrocution (220 V, 50 Hz, 60 sec) and electrical injury (220 V, 50 Hz, 60 sec) groups (n=8 per group). The animals in the fatal electrocution and electrical injury groups were deeply anesthetized with sodium pentobarbital prior to each treatment, in which the current was delivered via an anode connected to the left foreleg and a cathode to the right hindleg. The rats that survived were subsequently sacrificed by cervical dislocation. Control animals received cervical dislocation alone. Immunohistochemical analysis was performed to evaluate the protein expression of FABP1 and gastrin R in the testes. Sections were evaluated by digital image analysis. The expression levels of FABP1 and gastrin R were significantly increased following electrical injury, supported by an increase in the integrated optical density (IOD) when compared with that in the control group (P<0.05). However, no significant difference was found in FABP1 and gastrin R expression levels between the fatal electrocution and control groups. In summary, the protein expression levels of FABP1 and gastrin R were found to be significantly altered by electrical injury, suggesting that these two proteins may be important in underlying mechanisms of testicular injury during electrical injury. The findings indicate that such alterations would be reflected in abnormal testicular function.

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“…The plasma lipid profile in males is determined, among other factors, by the secretion level of sex hormones possessing antiatherogenic properties. A lot of studies proved the presence of direct correlation between the blood testosterone plus the dehydrotestosterone level and the HDL-C content [48]. Moreover, the high level of sex hormones correlates with decrease of the TAG content and the total cholesterol in the blood.…”

Section: Discussionmentioning

confidence: 99%

“…The increase of the LDL receptor activity and abundance may be a result of the homeostatic intracellular cholesterol feedback loop. In general, decrease in the intracellular cholesterol will upregulate the LDL receptor expression and activity, whereas increase in the intracellular cholesterol will downregulate the receptor [48]. Grape polyphenols were shown to decrease hepatic cholesterol concentrations; therefore, the liver compensates for this deficiency by upregulating the LDL receptor and the overall decrease in the plasma LDL concentrations occurs.…”

Section: Parametersmentioning

confidence: 99%