Effect of Sterilization on the Properties of a Bioactive Hybrid Coating Containing Hydroxyapatite

Abstract

The objective of this study was to evaluate the influence of sterilization on a hybrid coating obtained from a sol composed of alkoxysilane tetraethoxysilane (TEOS) and organoalkoxysilane methyltriethoxysilane (MTES) containing 10% (mass) of hydroxyapatite particles. The coating was obtained by dip coating, by applying two layers (protective/bioactive), which were cured at different temperatures (450°C and 60°C). The effects of sterilization on the superficial, electrochemical, bioactive, and mechanical properties of the coating were evaluated by performing different sterilization processes, namely, steam autoclave, hydrogen peroxide plasma, and ethylene oxide. Subsequently, the coating was characterized by using scanning electron microscopy (SEM/FEG), and FTIR measurements were performed to characterize the chemical structure. The bioactivity and degradability of the coating were analyzed by mass variation after immersion in SBF and X-ray diffraction (XRD) analysis. The electrochemical behavior was assessed by open circuit potential (OCP) and potentiodynamic polarization curves and the mechanical behavior by wear resistance. Results showed that all sterilization processes caused significant morphological changes in the hybrid coating. The autoclaved sample presented the highest structural chemical changes, and, consequently, the highest degradability, even though it had a superior bioactive behavior in relation to the other samples. In addition, the sterilization processes influenced the electrochemical behavior of the hybrid coating and altered the mechanical resistance to abrasion, thus presenting lower wear performance in relation to the nonsterilized sample.

Silver ion doped hydroxyapatite-coated titanium pins prevent bacterial colonization

“…In each group, eight implant samples were sonicated to lyse bacteria and used for bacterial count. Since the choice of sterilization methods may cause changes in the chemical structure of the coatings and their bioactivity behavior,[ 15 ] coated titanium pins were sterilized with ethylene oxide. Pressurized steam was used for the sterilization of uncoated pins.…”

Bench-to-bedside: Feasibility of nano-engineered and drug-delivery biomaterials for bone-anchored implants and periodontal applications

“…Baldin et al. reported the application of 3 different methods (steam autoclave, hydrogen peroxide plasma and ethylene oxide) for the sterilization of bioactive hybrid coatings containing hydroxyapatite particles; their study demonstrated negative consequences regarding the morphological structure and mechanical resistance for all the methods applied compared to non-sterilized samples [ 77 ]. Similarly, another study showed the application of 5 different methodologies for the sterilization of polymeric coatings, and they included autoclave, ultraviolet light, dry heat sterilization, ethylene oxide and gamma rays.…”