Effect of Stemona curtisii root extract on P-glycoprotein and MRP-1 function in multidrug-resistant cancer cells

Limtrakul, Pornngarm; Siwanon, S.; Yodkeeree, Supachai; Duangrat, Chadarat

doi:10.1016/j.phymed.2007.03.006

Cited by 36 publications

(20 citation statements)

References 28 publications

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“…3a-d). Our previous report demonstrated that Stemona extract did not influence MDR-mediated multidrug resistance protein 1 (MRP-1) but P-gp, 24) while PTX is a P-gp specific substrate that differ from Dox and Vin which are the substrates of the MRP-1 as well. 25,26) These might be the reason why the reversing property of stemofoline and its derivatives including OH-A1, NH-B6 and NH-D6 in PTX-treated cells was greater than in Dox-, and Vin-treated cells.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of P-Glycoprotein Mediated Multidrug Resistance by Stemofoline Derivatives

Umsumarng

Pintha

Pitchakarn

et al. 2013

CHEMICAL & PHARMACEUTICAL BULLETIN

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Resistance to chemotherapy in cancer patients has been correlated to the overexpression of the ATPbinding cassette (ABC) drug transporters including P-glycoprotein (P-gp) that actively efflux chemotherapeutic drugs from cancer cells. We examined the mutidrug resistance reversing property of stemofoline derivatives in drug-resistance human cervical carcinoma (KB-V1) and human leukemic (K562/Adr) cell lines that overexpress P-gp. Didehydrostemofoline and eleven of its derivatives were synthesized and the cytotoxicity and their effect on doxorubicin, vinblastine and paclitaxel sensitivity in drug resistant (KB-V1 and K562/ Adr) and drug sensitive (KB-3-1 and K562) cell lines by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay were determined. We found that three out of the twelve stemofoline derivatives including OH-A1, NH-B6 and NH-D6 showed commitment efficiency to increase sensitivity to doxorubicin, vinblastine and paclitaxel in KB-V1 cells and increase sensitivity to doxorubicin, and paclitaxel in K562/Adr cells whereas the effects have not been seen in their parental sensitive cancer cell lines (KB-3-1 and K562). These results indicate that stemofoline derivatives reversed P-gp-mediated multidrug resistance in vitro, and thus could be developed as effective chemosensitizers to treat multidrug-resistant cancers. The molecular mechanism of modulation of P-gp would be further determined.

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Section: Discussionmentioning

confidence: 99%

Inhibition of P-Glycoprotein Mediated Multidrug Resistance by Stemofoline Derivatives

Umsumarng

Pintha

Pitchakarn

et al. 2013

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…18 In recent years, extracts and compounds isolated from herbaceous plant, such as Stemona curtisii root extract and Schisandrol A from Schisandra chinensis, were tested for their potential ability to modulate the MDR phenotype, and many of them have been identified to be effective in the treatment of multidrug resistant cancers. 19,20 However, these agents still need more investigation to confirm the effectiveness and safety before clinical use.…”

Section: Discussionmentioning

confidence: 99%

Proteasome inhibitor MG132 reverses multidrug resistance of gastric cancer through enhancing apoptosis and inhibiting P-gp

Zhang

Shi²,

Li³

et al. 2008

Cancer Biology & Therapy

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Multidrug resistance (MDR) is a major impediment to the effective chemotherapy of many human malignancies. Although much effort has been devoted to develop new drugs for overcoming MDR, until now, still no useful method of reversing MDR, suitable for clinical use, has emerged from this large quantity of work. Some researchers have reported that proteasome inhibitors could induce apoptosis in a variety of cancer cells. In the present study, we found that, in vincristine-resistant human gastric cancer cell line SGC7901/VCR, proteasome inhibitor MG132 was an effective inducer of apoptosis, and also had the capacity of downregulating the expression of anti-apoptotic Bcl-2 and MDR1 (P-gp), by which MG132 resensitized tumor cells to the apoptosis induced by anticancer drugs. Data presented by drug sensitivity assay further demonstrated that MG132 could reverse the resistant phenotype of gastric cancer cells effectively through both enhancing druginduced apoptosis and inhibiting P-gp. The further study of the effectiveness and safety of proteasome inhibitor in vivo may be helpful for developing a new possible strategy to treat gastric cancer MDR.

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“…These latter properties are most likely associated with the ability of these alkaloids to inhibit insect acetylcholinesterase (AChE) (Brem et al, 2002;Jiwajinda et al, 2001;Kaltenegger et al, 2003;Kongkiatpaiboon et al, 2013Kongkiatpaiboon et al, , 2012Mungkornasawakul et al, 2009Mungkornasawakul et al, , 2004Phattharaphan et al, 2010;Sanguanpong and Hummel 2008;Sakata et al, 1978;Tang et al, 2008). While other alkaloids have shown oxytocin antagonism (Phuwapraisirisan et al, 2006), nitric oxide inhibition (Hosoya et al, 2011) and the ability to inhibit P-glycoprotein in multi-drug-resistant cancer cell lines (Chanmahasathien et al, 2011a(Chanmahasathien et al, , 2011bLimtrakul et al, 2007;Umsumarng et al, 2013). In the present paper, we describe the results of our phytochemical investigation of the roots of S. javanica collected from Alas Purwo in Indonesia.…”

Section: Introductionmentioning

confidence: 99%

Alkaloids from the roots of Stemona javanica (Kunth) Engl. (Stemonaceae) and their anti-malarial, acetylcholinesterase inhibitory and cytotoxic activities

Ramli

Pudjiastuti

Tjahjandaric

et al. 2015

Phytochemistry Letters

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. Alkaloids from the roots of Stemona javanica (Kunth) Engl. (Stemonaceae) and their anti-malarial, acetylcholinesterase inhibitory and cytotoxic activities. Phytochemistry Letters, Alkaloids from the roots of Stemona javanica (Kunth) Engl. (Stemonaceae) and their anti-malarial, acetylcholinesterase inhibitory and cytotoxic activities AbstractTwo new protostemonine-type alkaloids, javastemonine A and B (3 and 4) have been isolated from the root extracts of Stemona javanica together with four known Stemona alkaloids, 13-demethoxy-11(S*),12(R*)-dihydroprotostemonine (1), isoprotostemonine (2), protostemonine and isomaistemonine. The structures and relative configurations of the new alkaloids were determined by spectroscopic analysis. The alkaloids 1 and 2 and protostemonine showed moderated antiplasmodial activities against the Plasmodium falciparum strains, TM4 (IC50 values of 17.7 ± 3.7, 16.8 ± 5.4, 16.0 ± 4.2 μg/mL, respectively) and K1 (IC50 values of 16.8 ± 3.1, 14.1 ± 3.7, 11.9 ± 3.3 μg/mL, respectively). These compounds showed no significant cytotoxicities against KB or Vero cells or acetylcholinesterase inhibitory activities. Disciplines Medicine and Health Sciences | Social and Behavioral Sciences Publication DetailsRamli, R., Pudjiastuti, P., Tjahjandaric, T. S., Lie, W., Rattanajak, R., Kamchonwongapaisan, S. & Pyne, S. G. values of 17.7 ± 3.7, 16.8 ± 5.4, 16.0 ± 4.2 µg/mL, respectively) and K1 (IC 50 values of 16.8 ± 3.1, 14.1 ± 3.7, 11.9 ± 3.3 µg/mL, respectively). These compounds showed no significant cytotoxicities against KB or Vero cells or acetylcholinesterase inhibitory activities.2

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Effect of Stemona curtisii root extract on P-glycoprotein and MRP-1 function in multidrug-resistant cancer cells

Cited by 36 publications

References 28 publications

Inhibition of P-Glycoprotein Mediated Multidrug Resistance by Stemofoline Derivatives

Inhibition of P-Glycoprotein Mediated Multidrug Resistance by Stemofoline Derivatives

Proteasome inhibitor MG132 reverses multidrug resistance of gastric cancer through enhancing apoptosis and inhibiting P-gp

Alkaloids from the roots of Stemona javanica (Kunth) Engl. (Stemonaceae) and their anti-malarial, acetylcholinesterase inhibitory and cytotoxic activities

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