Effect of Stem Cell Therapy on Adriamycin Induced Tubulointerstitial Injury

Abstract

Background and Objectives: It was postulated that adriamycin (ADR) induce renal tubulointerstitial injury. Clinicians are faced with a challenge in producing response in renal patients and slowing or halting the evolution towards kidney failure. The present study aimed at investigating the relation between the possible therapeutic effect of human mesenchymal stem cells (HMSCs), isolated from cord blood on tubular renal damage and their distribution by using ADR induced nephrotoxicity as a model in albino rat. Methods and Results: Thirty three male albino rats were divided into control group, ADR group where rats were given single intraperitoneal (IP) injection of 5 mg/kg adriamycin. The rats were sacrificed 10, 20 and 30 days following confirmation of tubular injury. In stem cell therapy group, rats were injected with HMSCs following confirmation of renal injury and sacrificed 10, 20 and 30 days after HMSCs therapy. Kidney sections were exposed to histological, histochemical, immunohistochemical, morphometric and serological studies. In response to SC therapy, vacuolated cytoplasm, dark nuclei, detached epithelial lining and desquamated nuclei were noticed in few collecting tubules (CT). 10, 20 and 30 days following therapy. The mean count of CT showing desquamated nuclei and mean value of serum creatinine revealed significant difference in ADR group. The mean area% of Prussian blue+ve cells and that of CD105 +ve cells measured in subgroup S1 denoted a significant increase compared to subgroups S2 and S3. Conclusions: ADR induced tubulointerstitial damage that regressed in response to cord blood HMSC therapy.

Histological study on the protective effect of endogenous stem-cell mobilization in Adriamycin-induced chronic nephropathy in rats

“…These results were consistent with Zickri et al [ 5 ] who demonstrated that a single intravenous injection of ADR in rats resulted in a tubulo-interstitial injury in the form of vacuolated cytoplasm, dark nuclei in the cells lining most tubules, detached epithelial cells, and desquamated dark nuclei in the lumina of some tubules. It was postulated that ADR-induced tubular atrophy may be related to the formation of reactive metabolites that attack cell membranes and result in peroxidation of polyunsaturated fatty acids.…”

“…In Group II (ADR group), the rats received a single intravenous injection (tail vein) of ADR at a dose of 5 mg/kg of body weight [ 5 ].…”

Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease?

“…In Group II (Adriamycin group), the rats received a single intravenous injection (tail vein) of Adriamycin at a dose of 5 mg/kg of body weight (Montilla et al, 1997) and 1 ml saline 1 week after adriamycin injection. In Group III (adipose‐derived stem cells group), rats received ADMSCs (2 × 10 6 cells suspended in 1 ml saline) 1 week after the Adriamycin injection (Zickri et al, 2012). In Group IV (G‐CSF group), the rats received Adriamycin by the same route and the same dose as Group II, and then G‐CSF was given 2 h after the Adriamycin injection.…”

Nephroprotective Effect of Mesenchymal Stem Cells in Therapy of Kidney Disease Induced by Toxicant

“…Scr26 studies[10][11][12][13][14][15][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36] was included to assess the effect of MSCs on Scr, three for 2 days, four for 3-day, ve for 4 days, six for 5-day, seven for 6-8 days, ten for 10-15 days, six for 28-30 days, and six for ≥ 42days, and the results showed that the difference between MSCs treatment group and control group was…”