Effect of Steaming, Blanching, and High Temperature/High Pressure Processing on the Amino Acid Contents of Commonly Consumed Korean Vegetables and Pulses

Kim, Su-Yeon; Kim, Bomin; Kim, Jung-Bong; Shanmugavelan, Poovan; Kim, Heon-Woong; Kim, Soyoung; Kim, Se-Na; Cho, Young-Sook; Choi, Han‐Seok; Park, Ki-Moon

doi:10.3746/pnf.2014.19.3.220

Cited by 12 publications

(5 citation statements)

References 13 publications

(19 reference statements)

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“…The amino acid composition is related to the chemical, physical, and nutrient property of food materials, and may be changed as a result of temperature, pressure, and oxidation treatments (Kim and others ; Wang and others ). The composition of amino acids expressed as g/100 g protein at different temperatures was evaluated as shown in Table .…”

Section: Resultsmentioning

confidence: 99%

Thermal Stability Improvement of Rice Bran Albumin Protein Incorporated with Epigallocatechin Gallate

Zhou

Liu

et al. 2017

Journal of Food Science

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Rice bran albumin protein (RAP) is sensitive to thermal changes and tends to degrade when exposed to high-temperature processing. In this work, RAP-epigallocatechin-3-gallate (EGCG) complex (RAPE) was prepared and the thermal stability was evaluated. Fluorescence results showed that EGCG could interact with RAP with a binding number n of 0.0885:1 (EGCG:RAP, w/w) and a binding constant K of 1.02 (± 0.002) ×10 /M, suggesting both hydrogen bonding and van der Waals forces played an important role. FTIR analysis demonstrated that EGCG could induce secondary structural changes in RAP above a ratio of 1.6:1 (EGCG:RAP, w/w). Interestingly, the secondary structure changes of RAPE at different temperatures (25, 50, 60, 70, and 80 °C) were inhibited compared with that for RAP, suggesting RAPE was more resistant and stable to the heat treatment. In addition, a dense porous structure of RAPE was achieved due to the EGCG binding after thermal treatment. Furthermore, the T temperature of RAPE increased significantly from 64.58 to 74.16 °C and the enthalpy also increased from 85.53 to 138.52 J/g with a mass ratio increasing from 0 to 3.2 (EGCG:RAP, w/w), demonstrating the thermal stability of RAPE. In addition, the valine, methionine, and lysine content in RAPE were significantly higher than RAP following 80 °C treatment for 20 min (P < 0.05), exhibiting enhanced amino acid profiles, which might be due to EGCG-RAP interactions and microenvironment changes around relevant amino acids. These findings demonstrate that EGCG has the potential to improve the thermal stability of sensitive proteins and is beneficial for usage in the food industry.

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Section: Resultsmentioning

confidence: 99%

Thermal Stability Improvement of Rice Bran Albumin Protein Incorporated with Epigallocatechin Gallate

Zhou

Liu

et al. 2017

Journal of Food Science

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“…The levels of total glutathione content in tomato roots extracted in 2 mL of 0.1 M sodium phosphate buffer containing 5 mM EDTA (pH 7.5) were determined on the basis of the reaction of GSH with DTNB [5,50-dithio-bis (2-nitrobenzoic acid)], which produces the yellow derivative 50-thio-2-nitrobenzoic acid (TNB), according to the procedure described by Yu et al 37. The methionine level in tomato roots was determined using an Automatic Amino Acid Analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan) after hydrolysis with HCl, and an external standard was used to calculate the concentration in tomato roots3839. An Elementary Analysis System (Vario EL Cube, Germany) was used to determine the S 0 level in tomato roots, according to the general procedure for elemental analyzers (JY/T017-1996, China).…”

Section: Methodsmentioning

confidence: 99%

Physiological response and sulfur metabolism of the V. dahliae-infected tomato plants in tomato/potato onion companion cropping

Zhou

et al. 2016

Sci Rep

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Companion cropping with potato onions (Allium cepa var. agrogatum Don.) can enhance the disease resistance of tomato plants (Solanum lycopersicum) to Verticillium dahliae infection by increasing the expressions of genes related to disease resistance. However, it is not clear how tomato plants physiologically respond to V. dahliae infection and what roles sulfur plays in the disease-resistance. Pot experiments were performed to examine changes in the physiology and sulfur metabolism of tomato roots infected by V. dahliae under the companion cropping (tomato/potato onion). The results showed that the companion cropping increased the content of total phenol, lignin and glutathione and increased the activities of peroxidase, polyphenol oxidase and phenylalanine ammonia lyase in the roots of tomato plants. RNA-seq analysis showed that the expressions of genes involved in sulfur uptake and assimilation, and the formation of sulfur-containing defense compounds (SDCs) were up-regulated in the V. dahlia-infected tomatoes in the companion cropping. In addition, the interactions among tomato, potato onion and V. dahliae induced the expression of the high- affinity sulfate transporter gene in the tomato roots. These results suggest that sulfur may play important roles in tomato disease resistance against V. dahliae.

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“…Subsequently, the mixture was centrifuged for 20 min (4°C, 20,000 × g) and filtered with a filter (0.22 μm) for further analysis. Hepatic amino acid concentrations were measured using an LA8080 automatic amino acid analyser (Hitachi, Tokyo, Japan) following the method proposed by Kim et al ( 16 ).…”

Section: Methodsmentioning

confidence: 99%

Determination of the Effects of Duodenal Infusion Soy Protein Hydrolysate on Hepatic Glucose and Lipid Metabolism in Pigs Through Multi-Omics Analysis

Ding

Zhu

et al. 2022

Front. Nutr.

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High animal protein intake increases hepatic lipid deposition and the risk of diabetes. However, the effects of high plant protein (HPP) intake on glycaemic responses and hepatic lipid metabolism in healthy people, as well as the underlying mechanisms, remain unclear. The current study explored the metabolomic and transcriptomic responses in the livers of pigs to assess the effects of HPP intake on host glucose and lipid metabolism. Sixteen pigs were infused with sterile saline or soy protein hydrolysate (SPH; 70 g/day) through a duodenal fistula twice daily during a 15 days experimental period. Hepatic metabolomic and transcriptomic analyses were performed, and the serum and hepatic biochemical parameters were measured. The results revealed that SPH infusion decreased serum glucose, hepatic triglyceride (TG), total cholesterol and low-density lipoprotein cholesterol levels, while it increased serum urea and eight hepatic amino acid levels (P < 0.05). Hepatic metabolomics displayed that SPH treatment produced seven different metabolites, four of which were related to lipid metabolism and one was related to glucose metabolism. In particular, lower (P < 0.05) glycocholic acid and glucose 1-phosphate levels and higher (P < 0.05) phosphatidylethanolamine (PE), arachidonic acid, prostaglandin F2α, l-carnitine and indole-3 acetic acid levels were observed following SPH infusion. A further metabolic pathway enrichment analysis found that these differential metabolites were mainly enriched in pathways related to lipid and glucose metabolism. Hepatic transcriptomics also demonstrated that multiple genes related to glucose and lipid metabolism were affected by SPH (P < 0.05). Together, SPH infusion reduced the hepatic TG levels by accelerating fatty acid β-oxidation and inhibiting TG synthesis. In addition, SPH infusion reduced the serum glucose levels by promoting hepatic glucose uptake and glycolysis. This study's result demonstrated that HPP intake regulated glycaemic responses and hepatic lipid metabolism in pigs without increasing the risk of hepatic lipid deposition and hyperglycaemia.

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Effect of Steaming, Blanching, and High Temperature/High Pressure Processing on the Amino Acid Contents of Commonly Consumed Korean Vegetables and Pulses

Cited by 12 publications

References 13 publications

Thermal Stability Improvement of Rice Bran Albumin Protein Incorporated with Epigallocatechin Gallate

Thermal Stability Improvement of Rice Bran Albumin Protein Incorporated with Epigallocatechin Gallate

Physiological response and sulfur metabolism of the V. dahliae-infected tomato plants in tomato/potato onion companion cropping

Determination of the Effects of Duodenal Infusion Soy Protein Hydrolysate on Hepatic Glucose and Lipid Metabolism in Pigs Through Multi-Omics Analysis

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