Effect of Steamed Broccoli Juice (Brassica oleraceae L. var. italica) to the Serum Interleukin 8 Level in Colitis Murine Model

Abstract

Background: Ulcerative colitis (UC) is a bowel inflammation that happens in the colon and rectum. In UC there is an increased level of inflammatory mediators, one of which is interleukin-8 (IL-8), the main chemoattractant for polymorphonuclear (PMN) inflammatory cells causing inflammation to worsen. Broccoli (Brassica oleraceae L. var. italica) contains sulforaphane which can inhibit nuclear factor kappa B (NF-ĸB), resulting in the decrease of IL-8. This study was performed to identify the effect of steamed broccoli juice to the serum interleukin-8 level in murine model of colitis.Method: This study was a true experimental laboratory study using complete randomized design. Experimental animals being used were 28 murines with Balb/c strain and were divided into 4 intervention groups. Serum IL-8 level was measured by using enzyme-linked immunosorbent assay (ELISA) method. Data analysis was performed using analysis of variance (ANOVA) test, followed by multiple comparisons Tukey honest significant difference (HSD) test (a = 0.05) and p 0.05.Results: The highest mean of IL-8 level was found in the positive control group. This study also showed highly significant difference (p 0.001) of IL-8 level between positive control group and treatment group.Conclusion: Steamed broccoli juice could decrease serum interleukin-8 level in murine model of colitis.

Current Knowledge on the Preparation and Benefits of Cruciferous Vegetables as Relates to In Vitro, In Vivo, and Clinical Models of Inflammatory Bowel Disease

“…Similar to the findings from in vitro studies, broccoli has been reported to have anti-inflammatory, antioxidant, and prebiotic effects against IBD in animal models ( Table 2 [ 48 , 99 , 102 , 105 , 107 , 111 , 112 , 116 , 118 , 122 , 125 , [127] , [128] , [129] , [130] , [131] , [132] , [133] , [134] , [135] , [136] , [137] , [138] , [139] , [140] , [141] ] and Figure 1 ). Both the UC and CD models use chemical triggers or genetic knockouts.…”

“… Prebiotic, Anti-inflammatory mdr1a−/− mice [ 132 ] SFN Crude extract 4% 12 d NFkB, TNFɑ, IL10, MCP-1, and COX 2 No significance DSS-rat [ 102 ] N.R. Juice 1.5 mL 7 d IL8 Anti-inflammatory DSS mice [ 133 ] SFN, Lipids Edible nanoparticles mean diameter 32.4 nm 10 and 13 d AMPK, IL10, TNFɑ, IL17A and IFN-γ Anti-inflammatory DSS mice [ 116 ] SFN, Lipids Edible nanoparticles mean diameter 32.4 nm 10 d TNFɑ, IL6, and IL23 Anti-inflammatory Rag1−/− mice [ 116 ] Broccoli sprouts GLR, SFN Steamed and freeze-dried 10% (w/w) 34 d Bacillota, Pseudomonadota, Bacteroidota and Verrucomicrobiota; TNFɑ, IL1β and IL6 Prebiotic, Anti-inflammatory DSS mice [ 48 ] SFN, GLR Streamed 5% (w/w) 13 d microbial hydrolase, Bacillota Prebiotic DSS mice [ 105 ] SFN raw 10% (w/w) 2 wk E. coli and Helicobacter Prebiotic, Anti-inflammatory IL10-KO mice [ 134 ] Broccoli sprouts and seed GLR, Glucoiberin, Glucoerucin,...…”

“…Raw broccoli extract did not alleviate inflammation and oxidative stress in DSS mice [ 102 ], but interestingly, broccoli juice decreased serum IL8 concentrations to alleviate intestinal and systemic inflammation in DSS mice [ 133 ], again highlighting the importance of preparation’s effect on symptoms. Meanwhile, BDN increased AMPK to inhibit DCs and decreased mucosal inflammatory score.…”