Effect of starvation time on NO and N2O production during heterotrophic denitrification with nitrite and glucose shock loading

Wang, Sha; Zhao, Jian‐Qiang; Ding, Xiaoqian; Zhao, Rixiang; Huang, Ting; Lan, Lan; Nasry, Allama Al Naim Bin; Liu, Shuang

doi:10.1016/j.procbio.2019.07.023

Cited by 29 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Those four genera were: Gemmobacter , a poly‐hydroxybutyrate‐accumulating and denitrifying bacteria; 87 Clostridium , a known fermenter and VFA producer; 76 Lachnoclostridium , a known butyrate producer; 88 and Rhodopseudomonas , a highly metabolically versatile bacteria capable of H 2 production through N 2 fixation or poly‐hydroxybutyrate production 89 . The operational conditions applied in the F/M‐0.5 experiment were thus apparently those that favor the best microbial combination to produce the highest level of VFAs.…”

Section: Resultsmentioning

confidence: 99%

Thermal and alkaline pre‐treatments of inoculum halt methanogenesis and enable cheese whey valorization by batch acidogenic fermentation

de Almeida,

Mondini,

Bruant

et al. 2024

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BackgroundCarboxylates like volatile fatty acids (VFAs) can be produced by acidogenic fermentation (AF) of dairy wastes like cheese whey, a massive residue produced at 160.67 million m3 of which 42% are not valorized and impact the environment. In mixed‐culture fermentations, selection pressures can favor AF and halt methanogenesis. In this study, inoculum pre‐treatment was evaluated as a selective pressure for AF demineralized cheese whey in batches. Alkaline (NaOH, pH 8.0, 6 h) and thermal (90°C for 5 min, ice‐bath until 23°C) pre‐treatments, were tested with batch operations runs at initial pH 7.0 and 9.0, food‐to‐microorganism (F/M) ratios of 0.5 to 4.0 g COD g‐1 VS, and under pressurized and non‐pressurized headspace, in experiments duplicated in two different research institutes.ResultsAcetic acid was highly produced on both Unicamp and TU Delft samples (1.36 and 1.40 g CODAcOH L‐1, respectively), at the expense of methanogenesis by combining a thermal pre‐treatment of inoculum with a non‐pressurized batch operation started at pH 9.0. Microbial communities comprised of VFAs and alcohol producers, such as Clostridium, Fonticella, and Intestinimonas, and fermenters such as Longilinea and Leptolinea. Communities also presented the lipid‐accumulating and bulk and foaming Candidatus Microthrix and the metanogenic Methanosaeta regardless of no methane production. An F/M ratio of 0.5 g COD g‐1 VS led to the best VFA production of 1,769.4 mg L‐1.ConclusionOverall, inoculum thermal pre‐treatment, initial pH 9.0, and non‐pressurized headspace acted as a selective pressure for halting methanogenesis and producing VFAs, valorizing cheese whey via batch acidogenic fermentation.This article is protected by copyright. All rights reserved.

show abstract

Section: Resultsmentioning

confidence: 99%

Thermal and alkaline pre‐treatments of inoculum halt methanogenesis and enable cheese whey valorization by batch acidogenic fermentation

de Almeida,

Mondini,

Bruant

et al. 2024

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…At lower F/M ratios, the community was mainly composed of the same four genera, which altogether represented 84.3%, 77.6%, and 74.7% of the F/M-0.5, F/M-1, and F/M-2 populations, respectively (Figure 3 and 4). Those four genera were Gemmobacter , a poly-hydroxybutyrate-accumulating and denitrifying bactéria (Wang et al, 2019), Clostridium , a known fermenter and VFAs producer (Mockaitis et al, 2022), Lachnoclostridium , a known butyrate producer (Detman et al, 2021), and Rhodopseudomonas , a highly metabolically versatile bacteria capable of hydrogen production through nitrogen fixation or poly-hydroxybutyrate production (Ranaivoarisoa et al, 2019). The operational conditions applied in the F/M-0.5 experiment were thus apparently those that favor the best microbial combination to produce the highest level of VFAs.…”

Section: Resultsmentioning

confidence: 99%

Thermal and alkaline pre-treatments of inoculum halt methanogenesis and enables cheese whey valorization by batch acidogenic fermentation

Almeida

Mondini

Bruant

et al. 2023

Preprint

View full text Add to dashboard Cite

Carboxylates like volatile fatty acids (VFAs) can be produced by acidogenic fermentation (AF) of dairy wastes like cheese whey, a massive residue produced at 160.67 million m3of which 42% are not valorized and impact the environment. In mixed-culture fermentations, selection pressures are needed to favor AF and halt methanogenesis. Inoculum pre-treatment was studied here as elective pressure for AF demineralized cheese whey in batch processes. Alkaline (NaOH, pH 8.0, 6 h) and thermal (90 °C for 5 min, ice-bath until 23 °C) pre-treatments, were tested together with batch operations run at initial pH 7.0 and 9.0, food-to-microorganism (F/M) ratios of 0.5 to 4.0 g COD g-1VS, and under pressurized and non-pressurized headspace, in experiments duplicated in two institutes. Acetic acid was highly produced (1.36 and 1.40 g CODAcOH L-1) at the expense of methanogenesis by combining a thermal pre-treatment of inoculum with a non-pressurized batch operation started at pH 9.0. Microbial communities comprised of VFAs and alcohol producers, such asClostridium,Fonticella, andIntestinimonas, and fermenters such asLongilineaandLeptolinea. Communities also presented the lipid-accumulating and bulk and foamingCandidatus Microthrixand the metanogenicMethanosaetaregardless of no methane production. An F/M ratio of 0.5 g COD g-1VS led to the best VFA production of 1,769.38 mg L-1. Overall, inoculum thermal pre-treatment, initial pH 9.0, and non-pressurized headspace acted as a selective pressure for halting methanogen and producing VFAs, valorizing cheese whey via batch acidogenic fermentation.

show abstract

“…Specifically, in the IBAS, the average relative abundances of the aerobic denitrifiers Paracoccus, Rhodobacter, , and Hyphomicrobium were largely reduced in C1 compared to C0. The facultative anaerobic denitrifier Thermomonas and anaerobic denitrifier Azospira were the top two genera (8.5 ± 1.1% and 3.5 ± 5.9%, respectively) in C0, but their abundances were reduced to 0.4 ± 0.2% and 0.1 ± 0.2%, respectively, in C1 (Figure ; Table S4). Devosia, Lautropia, and Bdellovibrio were also significantly decreased in C1 compared to C0 ( p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…The facultative anaerobic denitrifier Thermomonas and anaerobic denitrifier Azospira were the top two genera (8.5 ± 1.1% and 3.5 ± 5.9%, respectively) in C0, but their abundances were reduced to 0.4 ± 0.2% and 0.1 ± 0.2%, respectively, in C1 (Figure ; Table S4). Devosia, Lautropia, and Bdellovibrio were also significantly decreased in C1 compared to C0 ( p < 0.05). As a result, the relative abundance of all denitrifiers was reduced from 19.4 ± 9.6 to 12.5 ± 2.9% in the IBAS but remained stable in the SRAS fish tank (10.4 ± 2.0% and 10.4 ± 2.4% in T0 and T1, respectively) and even increased from 10.1 ± 4.8% to 12.3 ± 3.0% in the SRAS assimilation reactor (Figure ; Table S4).…”

Section: Resultsmentioning

confidence: 99%

Integrating Microbial Protein Production and Harvest Systems into Pilot-Scale Recirculating Aquaculture Systems for Sustainable Resource Recovery: Linking Nitrogen Recovery to Microbial Communities

Deng

Dai

Song

et al. 2021

Environ. Sci. Technol.

View full text Add to dashboard Cite

In aquaculture, it is important to raise the nitrogen recovery efficiency (NRE) to improve sustainability. To achieve this, recovery of microbial protein (RMP), instead of nitrification/denitrification in conventional wastewater treatment, is a promising approach whose microbiological mechanisms must be characterized. Here, periodic RMP was conducted in an in situ biofloc-based aquaculture system (IBAS) and a separating assimilation reactor-based recirculating aquaculture system (SRAS). Kinetic analysis indicated that a microbial biomass level of 3 g L −1 was optimal for inorganic N removal, and excess biomass was harvested to improve the NRE. Unlike the IBAS, the SRAS eliminated the fluctuation in water quality caused by the RMP. Periodic RMP significantly increased the NRE to 44−57% by promoting the filamentous bacterium Herpetosiphon and suppressing anaerobic denitrifiers. Aerobic chemoheterotrophy was the main microbial metabolic process for energy. After RMP, nitrate reductase-encoded functional genes (napA and narG) significantly decreased, while nitrite reductase-encoded functional genes, especially nirK, significantly increased. Co-occurrence networks analysis indicated that the cooperation and competition among organic matter degraders, filamentous bacteria, nitrifiers, and denitrifiers determined the microbial protein yield. These results provide fundamental insights into the influence of the RMP on microbial communities and functions, which is important for realizing sustainable aquaculture.

show abstract

Effect of starvation time on NO and N2O production during heterotrophic denitrification with nitrite and glucose shock loading

Cited by 29 publications

References 37 publications

Thermal and alkaline pre‐treatments of inoculum halt methanogenesis and enable cheese whey valorization by batch acidogenic fermentation

Thermal and alkaline pre‐treatments of inoculum halt methanogenesis and enable cheese whey valorization by batch acidogenic fermentation

Thermal and alkaline pre-treatments of inoculum halt methanogenesis and enables cheese whey valorization by batch acidogenic fermentation

Integrating Microbial Protein Production and Harvest Systems into Pilot-Scale Recirculating Aquaculture Systems for Sustainable Resource Recovery: Linking Nitrogen Recovery to Microbial Communities

Contact Info

Product

Resources

About