Effect of Spinning and Additional Cross-Linking with Dialdehyde Starch or Aluminum Ions on the Digestibility and Quality of Casein-&lt;i&gt;Vicia faba&lt;/i&gt;Protein Isolate Mixtures

1. Utilisation of supplementary free L-lysine hydrochloride was estimated in growing chicks and compared to that of protein-bound lysine. The technique used is based on the oxidative catabolism of either free (U-14C)-L-lysine or the labelled lysine incorporated into yeast proteins. 2. The animals received a wheat-wheat gluten diet which was L-lysine-supplemented with either unlabelled yeast proteins or a mixture of synthetic amino acids simulating the yeast proteins or L-lysine hydrochloride alone. 3. At 13 and 15 days after hatching, expiry of (14C)--carbon dioxide was followed 8 h after dosing with the appropriate radiolabelled diet. After 4 h, 0.66% of the protein-bound and 5.3 to 5.7% of the free lysine radioactivity appeared as 14C--carbon dioxide. 4. It is concluded that under these conditions lysine from both sources was utilised more efficiently than had been assumed hitherto, protein-bound lysine being slightly better utilised than free lysine.

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Estimation of the endogenous N proportions in ileal digesta and faeces in¹⁵N-labelled pigs

Simon¹,

Bergner²,

Ig³

1987

Archiv für Tierernaehrung

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Four 40 kg castrated male pigs fitted with simple 'T' cannulas in the terminal ileum were given 15N-labelled ammonium salts, added to a low protein diet, for 6 days. Excretion of 15N in urine and faeces was monitored daily throughout the labelling and subsequent experimental periods. During the experimental period the pigs were given a diet based on wheat and fish meal, supplemented with varying levels of partially hydrolysed straw meal to give crude fibre contents ranging from 40 to 132 g/kg. After adaptation to the particular levels of straw meal, faeces and ileal digesta were collected during successive 24 h periods. N digestibility values were determined by the chromic oxide ratio method. The retention of 15N labelled non-specific N was 0.46 of the dose given. The validity of using urine values as a measure of 15N abundance in endogenous N was demonstrated by the similarity of 15N abundance in urine immediately before slaughter at the end of the experiment and in the digestive secretory organs thereafter. The average amount of endogenous N passing the terminal ileum was 3.4 g/day or 0.30-0.50 of total ileal N flow. This was not affected by dietary fibre level. The proportion of faecal N which was of endogenous origin was similar to that in ileal digesta, suggesting similar utilization of endogenous and residual dietary N by hindgut bacteria. Half the endogenous N entering the large intestine was reabsorbed there. Increasing dietary crude fibre from 40 to 132 g/kg increased faecal endogenous N excretion from 1.3 to 2.0 g/animal and day.

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Effect of Spinning and Additional Cross-Linking with Dialdehyde Starch or Aluminum Ions on the Digestibility and Quality of Casein-<i>Vicia faba</i>Protein Isolate Mixtures

Cited by 3 publications

References 9 publications

Digestibility of Processed Food Protein

Digestibility of Processed Food Protein

Utilisation of free and protein dietary lysine in chicks estimated with isotopes

Estimation of the endogenous N proportions in ileal digesta and faeces in¹⁵N-labelled pigs

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Effect of Spinning and Additional Cross-Linking with Dialdehyde Starch or Aluminum Ions on the Digestibility and Quality of Casein-<i>Vicia faba</i>Protein Isolate Mixtures

Cited by 3 publications

References 9 publications

Digestibility of Processed Food Protein

Digestibility of Processed Food Protein

Utilisation of free and protein dietary lysine in chicks estimated with isotopes

Estimation of the endogenous N proportions in ileal digesta and faeces in15N-labelled pigs

Contact Info

Product

Resources

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Estimation of the endogenous N proportions in ileal digesta and faeces in¹⁵N-labelled pigs