Effect of Spermatozoa upon Carbonic Anhydrase Activity of Rabbit Endometrium1

Collado, M. L.; Castro, Guadalupe; Hicks, Juan José

doi:10.1095/biolreprod20.4.747

Cited by 5 publications

(3 citation statements)

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“…, 2000; Hynninen et al. , 2004) because the expression of rabbit endometrial carbonic anhydrases can be induced by progesterone (Lutwak‐Mann, 1955; Falk & Hodgen, 1972) and sperm themselves can increase enzyme activity by interacting with the endometrium (Collado et al. , 1979).…”

Section: Discussionmentioning

confidence: 99%

“…We assume that under in vivo conditions, HCO 3 ) is not distributed steadily and small amounts of HCO 3 ) are not accessible to sperm at all domains within the uterus. We also have to take into account that other secretory proteins might alter sperm beat frequency and that carbonic anhydrases could be responsible for the supply of HCO 3 ) (Friedley & Rosen, 1975;Ge & Spicer, 1988;Karhumaa et al, 2000;Hynninen et al, 2004) because the expression of rabbit endometrial carbonic anhydrases can be induced by progesterone (Lutwak-Mann, 1955;Falk & Hodgen, 1972) and sperm themselves can increase enzyme activity by interacting with the endometrium (Collado et al, 1979). Male patients are infertile as a result of CBAVD and azoospermia (Boyle, 2003;Yankaskas et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

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Early activation of sperm by HCO3− is regulated hormonally in the murine uterus

Mannowetz

Wandernoth

Hornung

et al. 2010

International Journal of Andrology

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Sperm deposited in the female genital tract receive signals for capacitation. Past work indicates that HCO(3) (-) is the initiating signal that the female reproductive tract contains the HCO(3) (-) -permeant anion channel cystic fibrosis transmembrane conductance regulator (CFTR) and that mutations in CFTR cause subfertility in both sexes. In this study, we examined whether CFTR controls uterine HCO(3) (-) content and sperm responses to it. Both CFTR protein and mRNA were absent in prepubertal murine uterus, but appeared in pubertal and adult tissues. Thus, CFTR is upregulated during development. Uterine CFTR mRNA additionally increased upon induced oestrus, most abundantly in uterus body and distal horns. Uterine fluid of oestrous females contained two-, and nearly fourfold more HCO(3) (-) than that of dioestrous and prepubertal animals, correlating with increased CFTR expression. For sperm incubated in and recovered from prepubertal uteri, flagellar beat frequency was no different from that before incubation. However, for sperm recovered from dioestrous and oestrous uteri, beat frequency was two- and fourfold higher, respectively. Thus, uterine HCO(3) (-) content may have physiological consequences for sperm motility. The male reproductive tract showed no regional distributions or developmental dependence of CFTR expression. Although the sperm flagellum showed CFTR immunoreactivity, CFTR blockers GlyH-101 or CFTR(inh) -172 did neither diminish HCO(3) (-) -evoked increases in sperm motility nor protein tyrosine phosphorylation. Our results indicate that in the uterus, both CFTR expression and the supply of HCO(3) (-) are upregulated hormonally. We propose that these changes coordinate ovulation with increases in sperm motility and promote other components of capacitation by pathways that do not require CFTR in sperm.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Early activation of sperm by HCO3− is regulated hormonally in the murine uterus

Mannowetz

Wandernoth

Hornung

et al. 2010

International Journal of Andrology

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“…The possible participation of plasma membrane glycosyltransferase during maturation is being studied in our laboratory utilizing lectins and pretreated spermatozoa [6], to alter the cellto-cell interaction.…”

Section: Subcellular Epididymal Sialyltransferase 37mentioning

confidence: 99%

Microsomal and Plasma Membrane Sialyltransferase Activity in Rat Epididymis

Bernal

Mercado

Calzada

et al. 1983

Archives of Andrology

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3H-sialyl residues transfer by subcellular epididymal fractions [plasma membrane (PM), microsomes (MI), and mitochondria (MT)] to both endogenous and exogenous acceptors was studied. Their fraction purity was valued (S'nucleotidase and glucose 6-phosphatase activities). The glycoprotein or glycolipid N-acetyl neuraminyl transferase activity in microsomes were 5 times higher in caput than in cauda (624 to 125 pmoles/30 min/mg protein). PM activity also was higher in caput. In MT fraction, the activity was smaller.Two mechanisms related to spermatozoa glycoprotein changes during maturation are proposed: secretion to the lumen of molecules previously glycosilated in microsomes and transialylation to spermatozoa from membranal ectoenzymes localized on the surface of the epididymal epithelium.

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In vivo measurement of pH and CO2 levels in the uterus of sows through the estrous cycle and after insemination

López-Albors

Llamas-López

Ortuño

et al. 2021

Sci Rep

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The pH–CO2–HCO3− system is a ubiquitous biological regulator with important functional implications for reproduction. Knowledge of the physiological values of its components is relevant for reproductive biology and the optimization of Assisted Reproductive Technologies (ARTs). However, in situ measurements of these parameters in the uterus are scarce or null. This study describes a non-invasive method for in situ time-lapse recording of pH and CO2 within the uterus of non-anesthetized sows. Animals were at three different reproductive conditions, estrous with no insemination and two hours after insemination, and diestrous. From pH and CO2 data, HCO3− concentration was estimated. The non-invasive approach to the porcine uterus with novel optical probes allowed the obtaining of in situ physiological values of pH, CO2, and HCO3−. Variable oscillatory patterns of pH, CO2 and HCO3− were found independently of the estrous condition. Insemination did not immediately change the levels of uterine pH, CO2 (%) and HCO3− concentration, but all the values were affected by the estrous cycle decreasing significantly at diestrous condition. This study contributes to a better understanding of the in vivo regulation of the pH-CO2-HCO3− system in the uterus and may help to optimize the protocols of sperm treatment for in vitro fertilization.

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Effect of Spermatozoa upon Carbonic Anhydrase Activity of Rabbit Endometrium1

Abstract: Duringthe preimplantation period, morphological and functional characteristics

Cited by 5 publications

References 0 publications

Early activation of sperm by HCO3− is regulated hormonally in the murine uterus

Early activation of sperm by HCO3− is regulated hormonally in the murine uterus

Microsomal and Plasma Membrane Sialyltransferase Activity in Rat Epididymis

In vivo measurement of pH and CO2 levels in the uterus of sows through the estrous cycle and after insemination

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