Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality

Meseguer, Marcos; Santiso, Rebeca; Garrido, Nicolás; García-Herrero, Sandra; Remohı́, José; Fernández, José Luis Fernández

doi:10.1016/j.fertnstert.2010.05.055

Cited by 172 publications

(124 citation statements)

References 30 publications

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“…The fact that the selection of spermatozoa with MSOME improves results in ICSI cycles suggests that the technique deselects a physiological abnormality in spermatozoa. One physiological abnormality shown to have a correlation with pregnancy outcome after ICSI is the level of DNA fragmentation [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

Wilding¹,

Coppola²,

Matteo

et al. 2010

J Assist Reprod Genet

117

100

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Purpose We used computer assisted sperm selection (MSOME) during cycles of intracytoplasmic sperm injection to test whether this technique improves results over traditional ICSI protocols. We also used the TUNEL assay to test whether MSOME could deselect physiologically abnormal spermatozoa. Methods Individual spermatozoa were examined with MSOME. Normal and abnormal spermatozoa were tested for the level of DNA fragmentation using TUNEL assay. In a prospective, randomized trial, patients were selected for standard ICSI, or IMSI techniques. We tested the two groups for biological and clinical parameters. Results 64.8% of spermatozoa, otherwise selectable for ICSI, were characterized by abnormalities after computer-assisted sperm analysis. These sperm were also characterized by an increase in the level of DNA fragmentation. We noted an increase in embryo quality, pregnancy and implantation rates after computerized sperm selection during ICSI procedures. Conclusions Computerised selection of spermatozoa during ICSI procedures deselects physiological abnormal spermatozoa and improves clinical results.

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Section: Introductionmentioning

confidence: 99%

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

Wilding¹,

Coppola²,

Matteo

et al. 2010

J Assist Reprod Genet

117

100

View full text Add to dashboard Cite

show abstract

“…The ability of human oocyte to repair sperm DNA damage, has not been fully elucidated, but some studies showed that the oocyte is equipped with mechanisms that can repair some of the paternal DNA abnormalities [25,31,47]. However, the ability to repair the oocyte depends on the quality and genetics of the oocyte.…”

Section: Introductionmentioning

confidence: 99%

The predictive value of high-magnification sperm morphology examination on ICSI outcomes in the presence of oocyte dysmorphisms

Setti

Braga

Figueira

et al. 2012

J Assist Reprod Genet

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Purpose To evaluate the relationship between oocyte dysmorphisms and IMSI outcomes Methods Data of IMSI cycles performed in 332 patients were included in this study. Patients were included only if presented more than four and less than 30 oocytes upon oocyte retrieval. Patients who underwent IMSI were matched, concerning female age and sperm parameters, with patients who underwent ICSI in the same period (n0332). The two groups (ICSI and IMSI) were compared with regard to treatment outcomes. The influence of IMSI on fertilization and embryo quality on D3 and D5, when oocyte dysmorphisms were present was analyzed. Results A total of 6444 oocytes were morphologically evaluated and injected. Regardless of the oocyte quality, IMSI performance was a determinant of the increased odds of development to high quality embryo on D3 (OR: 1.98; CI: 1.54-2.56) and D5 (OR: 3.27; CI: 1.61-6.66). Conclusions The selection of a morphologically normal spermatozoon under high-magnification is associated with increased embryo quality, regardless of the oocyte morphology. The injection of a spermatozoon, selected under highmagnification, into a morphologically normal oocyte leads to the highest probability of developing high quality embryos.

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“…[4][5][6][7][8][9] To date, no consensus has been achieved regarding the effect of these techniques on the DNA integrity of sperm. Several investigations have shown that sperm preparation techniques considerably reduce the level of DNA damage present in a semen sample, [4][5][6][7] while other studies have reported that these methods do not recover spermatozoa with lower incidence of DNA damage. 8 Some groups have reported that the DGC technique appreciably reduces the percentage of sperm with DNA damage, whereas the SUP method shows no significant improvement.…”

Section: Introductionmentioning

confidence: 99%

The ability of sperm selection techniques to remove single- or double-strand DNA damage

Enciso¹,

Iglesias²,

Galán³

et al. 2011

Asian J Androl

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A wide variety of techniques for the preparation of sperm are currently available, of which the most commonly employed are densitygradient centrifugation (DGC) and swim-up (SUP). To date, these methods appear to be effective in selecting functional sperm for assisted reproduction techniques (ART), but they may have negative effects on sperm DNA. In this study, the ability of these semen processing techniques to eliminate spermatozoa containing single-and double-strand DNA damage was assessed by the two-tailed comet assay and the sperm chromatin dispersion test in 157 semen samples from patients seeking assisted reproduction treatment. Our results indicated that SUP and DGC are equally efficient in eliminating spermatozoa containing double-strand DNA damage and sperm with highly damaged (degraded) DNA, as characterized by the presence of both single-and double-strand DNA breaks. However, DGC is more efficient than SUP in selecting spermatozoa that are free from single-strand DNA damage. Future studies should characterise the importance of the various types of DNA damage and examine the sperm processing protocols used in each laboratory to determine their ability to eliminate DNA damage and hence, prevent the potential transmission of genetic mutations via ART.

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Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality

Cited by 172 publications

References 30 publications

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

The predictive value of high-magnification sperm morphology examination on ICSI outcomes in the presence of oocyte dysmorphisms

The ability of sperm selection techniques to remove single- or double-strand DNA damage

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