Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer

Nelson, Louis S.; Steussy, Bryan; Morris, Cory; Krasowski, Matthew D.

doi:10.1186/s40064-015-1546-x

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…Heparinized plasma or serum samples were tested using the sFLC assay (FREELITE™, The Binding Site Ltd., Birmingham, UK) to measure κ and λ immunoglobulin FLC levels at either the Mayo Clinic or National Cancer Institute (NCI) 23 . Prior studies have shown the high intraclass correlation between FLC measures using fresh and stored serum and for the FREELITE™ kappa and lambda free light chains assay; both EDTA-plasma and lithium heparin-plasma can serve as acceptable substitutes for serum 30,31 . As previously described, the analytic sensitivity for the nephelometric FLC assay is 0.1 mg/L for a monoclonal κ or λ FLC 32 .…”

Section: Methodsmentioning

confidence: 99%

Association of elevated serumfree light chains with chronic lymphocytic leukemia and monoclonal B-cell lymphocytosis

et al. 2019

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Chronic lymphocytic leukemia (CLL) and its precursor, monoclonal B-cell lymphocytosis (MBL), are heritable. Serumfree light-chain (sFLC) measures are a prognostic factor for CLL, but their role in susceptibility to CLL is not clear. We investigated differences between sFLC measurements in pre-treatment serum from five groups to inform the association of sFLC with familial and sporadic CLL: (1) familial CLL ( n = 154), (2) sporadic CLL ( n = 302), (3) familial MBL ( n = 87), (4) unaffected first-degree relatives from CLL/MBL families ( n = 263), and (5) reference population ( n = 15,396). The percent of individuals having elevated monoclonal and polyclonal sFLCs was compared using age-stratified and age- and sex-adjusted logistic regression models. In age groups >50 years, monoclonal sFLC elevations were increased in sporadic and familial CLL cases compared to the reference population ( p ’s < 0.05). However, there were no statistically significant differences in sFLC monoclonal or polyclonal elevations between familial and sporadic CLL cases ( p ’s > 0.05). Unaffected relatives and MBL cases from CLL/MBL families, ages >60 years, showed elevated monoclonal sFLC, compared to the reference population ( p ’s < 0.05). This is the first study to demonstrate monoclonal sFLC elevations in CLL cases compared to controls. Monoclonal sFLC levels may provide additional risk information in relatives of CLL probands.

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Section: Methodsmentioning

confidence: 99%

Association of elevated serumfree light chains with chronic lymphocytic leukemia and monoclonal B-cell lymphocytosis

et al. 2019

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“…Complete blood count tests and measurements of serum IgA, IgM, IgG, IgG1, IgG2, IgG3, and IgG4 levels were conducted before and one month after vaccination. Routine immunophenotyping of peripheral blood lymphocytes, β2microglobulin, IL-6, complement components 3 and 4 (C3 and C4), lactate dehydrogenase (LDH), albumin, total protein, M protein, free light chains (FLC), IgG, IgM, IgA serum levels, plasmablast evaluation, serum pneumococcal antibody assessment, and IgG subclasses assessment were performed as described previously 9,25. Assessment of serum pneumococcal antibody was done before vaccination (on the vaccination day) and 30 days after vaccination, using a commercial ELISA test (ELIZEN Pneumococcus IgG Assay, Zentech, Belgium), which determines the concentration of anti-capsular-polysaccharide antibody specific for 23 pneumococcal serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, 33F).…”

Section: Methodsmentioning

confidence: 99%

<p>Immunogenicity And Safety Of The 13-Valent Pneumococcal Conjugate Vaccine In Patients With Monoclonal Gammopathy Of Undetermined Significance – Relationship With Selected Immune And Clinical Parameters</p>

Pasiarski¹,

Sosnowska‐Pasiarska²,

Grywalska³

et al. 2019

CIA

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PurposePatients with monoclonal gammopathy of undetermined significance (MGUS) have an increased risk of developing infections. Streptococcus pneumoniae vaccinations are recommended for immunocompromised patients, including patients with lymphoproliferative disorders such as MGUS. The objective of the study was to assess the immune response to the 13-valent pneumococcal conjugate vaccine (PCV13) in treatment-naive MGUS patients versus healthy subjects. All study groups were evaluated for the levels of specific pneumococcal antibodies, the levels of IgG and IgG subclasses, and selected peripheral blood lymphocyte subpopulations, including the proportion of plasmablasts before and after immunization.Patients and methodsA total of 22 previously untreated patients with MGUS and 15 healthy age- and sex-matched volunteers were included in the study. All participants were immunized with PCV13 Prevenar13 (Pfizer). The following parameters were assessed: 1) serum-specific pneumococcal antibody titers before and 30 days after vaccination, 2) percentage of plasmablasts, defined as CD19+/IgD−/CD27++, before and 7 days after vaccination, 3) serum total IgG and IgG1, IgG2, IgG3, IgG4 levels before and 30 days after vaccination.Results and conclusionPCV13 vaccination in MGUS patients is safe and effectively protects against S. pneumoniae infection. In unvaccinated individuals, vaccination should be carried out as soon as possible after diagnosis. It can protect patients against serious infectious complications, which can contribute to extending the time to progression and transformation into more aggressive diseases. PCV13 vaccination is more effective in MGUS patients with a lower concentration of M protein. Serum M protein concentration in patients diagnosed with MGUS may be a useful predictor of the effectiveness of vaccination.

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“…Multiple projects focused on various aspects of assay validation, modification, or discontinuation. These included validation of plasma as an acceptable specimen for k and l free light chain analysis, 34 comparison of diazo and vanadate oxidase direct bilirubin assays, 35 validation of hematopoietic progenitor count on automated hematology analyzer for use in stem cell transplant, 51 and introduction of lamellar bodies assay for fetal lung maturity testing. A transfusion medicine-related project involved innovative use of an inexpensive device to limit blood loss for Jehovah's Witness patients.…”

Section: Other Clinical Pathology Projectsmentioning

confidence: 99%

Using Focused Laboratory Management and Quality Improvement Projects to Enhance Resident Training and Foster Scholarship

et al. 2017

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Training in patient safety, quality, and management is widely recognized as an important element of graduate medical education. These concepts have been intertwined in pathology graduate medical education for many years, although training programs face challenges in creating explicit learning opportunities in these fields. Tangibly involving pathology residents in management and quality improvement projects has the potential to teach and reinforce key concepts and further fulfill Accreditation Council for Graduate Medical Education goals for pursuing projects related to patient safety and quality improvement. In this report, we present our experience at a pathology residency program (University of Iowa) in engaging pathology residents in projects related to practical issues of laboratory management, process improvement, and informatics. In this program, at least 1 management/quality improvement project, typically performed during a clinical chemistry/management rotation, was required and ideally resulted in a journal publication. The residency program also initiated a monthly management/informatics series for pathology externs, residents, and fellows that covers a wide range of topics. Since 2010, all pathology residents at the University of Iowa have completed at least 1 management/quality improvement project. Many of the projects involved aspects of laboratory test utilization, with some projects focused on other areas such as human resources, informatics, or process improvement. Since 2012, 31 peer-reviewed journal articles involving effort from 26 residents have been published. Multiple projects resulted in changes in ongoing practice, particularly within the hospital electronic health record. Focused management/quality improvement projects involving pathology residents can result in both meaningful quality improvement and scholarly output.

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Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer

Cited by 9 publications

References 24 publications

Association of elevated serumfree light chains with chronic lymphocytic leukemia and monoclonal B-cell lymphocytosis

Association of elevated serumfree light chains with chronic lymphocytic leukemia and monoclonal B-cell lymphocytosis

<p>Immunogenicity And Safety Of The 13-Valent Pneumococcal Conjugate Vaccine In Patients With Monoclonal Gammopathy Of Undetermined Significance – Relationship With Selected Immune And Clinical Parameters</p>

Using Focused Laboratory Management and Quality Improvement Projects to Enhance Resident Training and Foster Scholarship

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