Effect of Sparasis crispa Extract on 5α-Reductase Gene in Dermal Papilla Cells

Abstract

Sparasis crispa (Cauliflower Mushroom) is widely used as a cosmetic product to promote hair growth, and most research on mouse hair growth has been reported. However, the study of the effects of Sparasis crispa on dermal papilla (DP) cells, which play an important role in hair growth, is not well known. For this reason, the researchers studied the effect of Sparasis crispa on DP cells. The strong antioxidant activity of Sparasis crispa was confirmed by ABTS analysis. In MTS analysis, 2 mg/mL of Sparasis crispa was treated, reducing cell survival to 160.2% in DP cells and no cytotoxicity was observed at 1 mg/mL concentration. The level of mRNA expression of the BMP6, FGF7, FGF10, CTNNB1, SRD5A1, SRD5A2 involved in hair cycle and hair loss induction was measured as quantitative RT-PCR after the Sparasis crispa treatment. Significant change in mRNA expression of type Ⅰ 5 alpha-reductase could be identified if Sparasis crispa with a concentration of 0.5 mg/ml was treated in DP cells. Additionally, type Ⅱ 5 alpha-reductase mRNA expression was found to have an effect increasing to 1.38 fold. It is not yet known that the treatment of Sparasis crispa increases the expression of type Ⅰ and type Ⅱ 5 alpha-reductase mRNA in the DP cells, which can have an excellent treatment effect for Sparasis crispa male hair loss. However, Sparasis crispa increased the mRNA expression of BMP6, FGF7 and CTNNB1 to promote hair production. For this reason, the effect of Sparasis crispa treatment can help in the action, which the final hair generation cycle requires.