Effect of soybean protein on coprostanol production and cholesterol metabolism in cholesterol-fed rats.

Tanaka, Chizuko; Watanuki, Masaaki; Nozaki, Yoshihisa

doi:10.3177/jnsv.29.447

Cited by 8 publications

(4 citation statements)

References 24 publications

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“…Many works have been concerned with mechanism(s) by which dietary proteins affect plasma cholesterol levels (13)(14)(15). Several lines of evidence suggest that fecal steroid excretion is one of the main factors that affect plasma cholesterol (5,(16)(17)(18)(19)(20), and we have also presented results which support this suggestion (21). In the present study, we further examined the relationship between plasma cho lesterol level and fecal steroid excretion in obese and normal mice.…”

Section: Discussionsupporting

confidence: 75%

The effect of dietary protein source on plasma cholesterol level and fecal steroid excretion in obese mice.

Yamashita

Hayashi

1990

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Both goldthioglucose (GTG)-treated and the genetically obese (C57BL/6J ob/ob) mice were fed semisynthetic diets containing either soy protein isolate (SPI) or casein as a protein source, or laboratory chow. In GTG-induced obese mice, the plasma cholesterol level corre lated positively with their body weight. The level was highest in mice fed high-fat diet, and lowest in ones fed laboratory chow. No difference was observed between SPI and casein groups whether the diet was low-fat or high-fat. Thus, in the GTG-treated mice, SPI did not have a hypocho lesterolemic effect while dietary fat had a hypercholesterolemic effect, and laboratory chow contained some component(s) which can lower the plasma cholesterol level. Both neutral and acidic steroid contents in feces of the SPI group were not different from those of the casein group, and both groups of mice excreted a smaller amount of steroids than mice fed laboratory chow. Results of essentially the same tendency were obtained with normal mice regarding the effects of SPI and casein, although the degree of hypercholesterolemia was lower in high-fat-fed normal mice than in similarly fed GTG-treated mice. These results strengthened the inverse correlation between the amount of fecal steroids and the plasma cholesterol level upon feeding various proteins, indicating that the former is one of the important factors that determine the latter. The ob/ob mice showed a marked hypercholesterolemia irrespective of the kind of diet. The amount of fecal steroids was highest in the laboratory chow group and lowest in the casein group. This indicates that some factor(s) other than fecal steroid excretion is dominantly responsible for their hyper cholesterolemia.

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Section: Discussionsupporting

confidence: 75%

The effect of dietary protein source on plasma cholesterol level and fecal steroid excretion in obese mice.

Yamashita

Hayashi

1990

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…The formation of hyodeoxycho lic acid from cholic acid by microflora was also increased. The stimulation of coprostanol formation due to undigested soybean protein has been reported by other groups (5,28). The soybean protein used in the present study contained very little saponin (less than 4g/kg).…”

Section: Fecal Excretion Of Cholesterol and Its Metabolitessupporting

confidence: 53%

Effects of dietary fish protein, soybean protein and casein on cholesterol turnover in rats.

Iritani

Rei

Fujita

et al. 1985

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThe effects of dietary fish, soybean protein and casein on cholesterol turnover were compared in rats. After the injection of [14C]cholesterol into the rats, the specific activities of radioactive choles terol in feces were followed for 4 weeks. The cholesterol half-lives calculated from the decay curves of the specific activities were 14.7 and 14.6 days in rats fed fish protein and soybean protein , respectively. These were shorter than the half-life (17.4 days) in casein-fed controls. The fish and the soybean protein feedings significantly increased the fecal ex cretions of cholesterol and coprostanol, respectively , and lowered the plasma cholesterol level, as compared with casein feeding. In addition, both fish and soybean protein feedings also increased the excretion of bile acids. The stimulation of cholesterol metabolism and the increased excretions of cholesterol and its metabolites by feeding fish or soybean protein appear to play important roles in the hypocholesterolemic effects.

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“…After absorption, further modi fication of the steroid may occur in the liver (89) . Research with steroid biotransformations by intestinal bacteria has emphasized the three major classes of steroids which pass through the GI tract; these are the bile acids (43,78,92,94,103), cholesterol (18,39,143,147), and steroid hormones (14,16,154). Although this review will almost exclusively deal with bacteria, it should be noted that protozoa and fungi can also play an important role in steroid biotransformations (21,60,121).…”

Section: Bacterial Transformations Of Steroids In the Gastrointestinamentioning

confidence: 99%

“…The net result would be an increased circulating bile acid pool size. Higher bile acid levels would also directly favor the absorption of cholesterol from the intestine(147).Tanaka et al(143) found that fecal bacteria from rats fed soy bean protein diets demonstrated an increased ability to produce coprostanol when compared to rats receiving casein as a protein source. The observed increase in biohydrogenation was attributed to soybean pro tein's ability to maintain higher levels of coprostanol-generating bac teria or bacteria with higher metabolic rats.…”

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confidence: 99%

C-10(19) oxidation of vitamin D by anaerobic gastrointestinal bacteria

Gardner

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Importance and implications Bile Cholesterol Other biologically important steroids 28 Vitamin D Vitamin D Importance and implications Metabolism Biological activity Enterohepatic cycling of vitamin D Requirements and toxicity SECTION I. FACTORS AFFECTING THE PRODUCTION OF 5(E) 19-NOR-lO-KETO-VITAMIN D3 BY RUMEN BACTERIA 46 MATERIALS AND METHODS 49 General 49 Steroids 49 Enzymes 50 Extraction and Chromatography 50 Preparation of Media 51 Assay Conditions 52 iii Rumen Fluid Studies 52 Oxygen studies 53 Preincubation treatments Mixed Culture Studies Filtration Time study Glucose inhibition Isolation of Clostridium sp. Strain A264 Pure Culture Studies Glucose inhibition studies Cysteine Studies RESULTS Rumen Fluid Studies BHI Broth Culture Studies DISCUSSION 62 LITERATURE CITED SECTION II. THE BIOLOGICAL ASSESSMENT OF VITAMIN Dg METABOLITES PRODUCED BY RUMEN BACTERIA 80 Biological Evaluation Preparation "of Bacterial Culture Medium Isolation of Clostridium sp. Strain A264 Rumen Fluid Assay Conditions Extraction and Chromatography RESULTS 91

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Effect of soybean protein on coprostanol production and cholesterol metabolism in cholesterol-fed rats.

Cited by 8 publications

References 24 publications

The effect of dietary protein source on plasma cholesterol level and fecal steroid excretion in obese mice.

The effect of dietary protein source on plasma cholesterol level and fecal steroid excretion in obese mice.

Effects of dietary fish protein, soybean protein and casein on cholesterol turnover in rats.

C-10(19) oxidation of vitamin D by anaerobic gastrointestinal bacteria

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