Effect of soybean milk fermented with <i>Lactobacillus plantarum</i> HFY01 isolated from yak yogurt on weight loss and lipid reduction in mice with obesity induced by a high-fat diet

Li, Chong; Liu, Huilin; Yang, Jiao; Mu, Jianfei; Wang, Ranran; Zhao, Xin

doi:10.1039/d0ra06977a

Cited by 30 publications

(24 citation statements)

References 58 publications

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“…Gene expression was calculated using the 2 −∆∆CT method. 15 The primer sequences are listed in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Silymarin Against D-Gal/LPS-Induced Organ Damage and Inflammation in Mice

Wang¹,

Yang²,

Gou³

et al. 2021

DDDT

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Aim Silymarin contains various flavonoids and exhibits antioxidative, anti-inflammatory, and anticancer effects, in addition to other pharmacological properties. This study explored the alleviating effect of silymarin on multiple-organ damage induced by D-galactose/lipopolysaccharide in Kunming mice. Methods Kunming mice were injected intraperitoneally with D-galactose (30 mg/kg·BW)/LPS (3 μg/kg·BW) and then treated using silymarin with different doses (75 mg/kg·bw and 150 mg/kg·bw) via intragastric administration. Changes in organ indexes, pathological changes, liver-function index, biochemical indexes, molecular biological indexes, and genes related to the oxidation and inflammation of main organs were evaluated. Results After the mice were treated with silymarin, their body weight showed no significant change, and the liver, kidney, and lung indexes of the treated mice were higher than those of the model group; meanwhile, the corresponding histopathological formation was reduced. Compared with the model group, the silymarin-treated group showed reductions in ALT, AST, and liver function indexes in the mouse serum. Silymarin treatment also increased the SOD, CAT, GSH, GSH-Px, T-AOC, IL-10, and IL-12 levels, as well as reduced the MDA, NO, IL-6, IL-1β, TNF-α, IFN-γ levels in the mouse serum and liver tissues. In addition, quantitative polymerase chain reaction analysis indicated that the mRNA expression levels of SOD1, SOD2, CAT, GSH-Px, IL-10, Nrf2, HO-1, NQO1, Trx , and IκB-α were higher in the liver tissue of the silymarin-treated mice than in those of the model group; meanwhile, the mRNA expression levels of IL-6, IL-1β, TNF-α, IFN-γ, NF-κB, NLRP3, COX2 , and p38 were lower than those in the model group. Conclusion Silymarin, which exhibits antioxidative and anti-inflammatory effects, can alleviate the liver, lung, and kidney damage induced by D-galactose/lipopolysaccharide. High-dose (150 mg/kg·bw) silymarin can more effectively inhibit organ damage, compared with low-dose silymarin (75 mg/kg·bw) in Kunming mice.

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“…Gene expression was calculated using the 2 −∆∆CT method. 15 The primer sequences are listed in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Silymarin Against D-Gal/LPS-Induced Organ Damage and Inflammation in Mice

Wang¹,

Yang²,

Gou³

et al. 2021

DDDT

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“…All mice (Kunming, male, 6-week-old) were fed a standard diet ( Table 4 ) [ 17 ] and provided with drinking water ad libitum. After adapting to the new environment for 1 week, 4 groups of mice were formed, with 10 mice in each group.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Inflammatory Effects of Nelumbo nucifera Gaertn. Leaves

Yang

et al. 2021

Oxidative Medicine and Cellular Longevity

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This study is aimed at identifying the bioactive components in lotus leaf flavonoid extract (LLFE) and analyzing the antioxidant and anti-inflammatory activities of LLFE in vitro and in vivo. The flavonoids in LLFE were determined by UHPLC-MS/MS. The effect of LLFE on damaged 293T cells (H2O2, 0.3 mmol/L) was determined by MTT assay, and the activity of antioxidant enzymes was measured by kits. We studied the antioxidant and anti-inflammatory effects of LLFE on D-Gal/LPS (30 mg/kg·bw and 3 μg/kg·bw)-induced aging mice. We also evaluated the main organ index, pathological changes in the liver, lung, and kidney, liver function index, biochemical index, cytokine level, and mRNA expression level in serum and liver. The results showed that LLFE contains baicalein, kaempferol, kaempferid, quercetin, isorhamnetin, hyperoside, lespenephryl, and rutin. LLFE reduced the oxidative damage sustained by 293T cells, increased the levels of SOD, CAT, GSH, and GSH-Px, and decreased the level of MDA. The animal studies revealed that LLFE reduced oxidative damage and inflammation in injured mice, inhibited increases in AST, ALT, MDA, and NO, increased SOD, CAT, GSH, and GSH-Px levels, upregulated anti-inflammatory cytokines IL-10 and IL-12, and downregulated proinflammatory cytokines IL-6, IL-1β, TNF-α, and IFN-γ. Furthermore, the expression of antioxidant- and anti-inflammatory-related mRNA was consistent with the above results.

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“…Carrageenan can induce thrombosis-related intravascular inflammation in the tail of mice, resulting in mixed thrombi filling in the venules, arterioles, and capillaries and causing ischemic necrosis of the tail tissue. The black tails of mice were observed intuitively (15). Therefore, the extent of the black tail in mice is an important experimental index to intuitively judge the degree of thrombosis.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the lactic acid bacteria isolated from yak yogurt in pastoral areas are very different from the commonly-used commercial lactic acid bacteria isolated from general yogurt (11,12). Studies have demonstrated that lactic acid bacteria isolated from yak yogurt show better colonization in the intestine, as well as good preventive and interventive effects on colitis and gastritis (13,14) and good regulatory effects on hyperlipidemia (15). As such, these lactic acid bacteria have good biological activity in the body.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus plantarum HFY05 Attenuates Carrageenan-Induced Thrombosis in Mice by Regulating NF-κB Pathway-Associated Inflammatory Responses

Zeng

Tan

et al. 2022

Front. Nutr.

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In this study, a carrageenan-induced thrombus model was established in mice to observe the ability of Lactobacillus plantarum KFY05 (LP-KFY05) to inhibit thrombosis through an NF-κB-associated pathway. Biochemical analysis, microscopical observations, quantitative polymerase chain reactions (qPCR) and western blot analysis were used to examine relevant serum and tissue indexes, and the composition of intestinal microorganisms was determined by examining the abundance of microorganisms in feces. The results showed that LP-KFY05 could markedly reduce the degree of black tail in thrombotic mice; increase the activated partial thromboplastin time (APTT); and decrease the thrombin time (TT), fibrinogen (FIB) level, and prothrombin time (PT). LP-KFY05 could also reduce tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) levels in sera and renal tissues of thrombotic mice. Hematoxylin and eosin staining showed that LP-KFY05 could alleviate renal tissue lesions and tail vein thrombosis. qPCR results showed that LP-KFY05 could down-regulate nuclear factor kappa-B (NF-κB) p65, IL-6, TNF-α, and interferon γ (IFN-γ) mRNA expression in renal tissues, as well as NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin mRNA expression in tail vein vascular tissues of thrombotic mice. Western blot analysis showed that LP-KFY05 also down-regulated NF-κB protein expression in renal and tail vein vascular tissues of thrombotic mice. Lastly, LP-KFY05 increased the abundances of Bacteroidetes, Lactobacillus, and Bifidobacterium, as well as decreased the abundance of Firmicutes. These results show that LP-KFY05 can reduce inflammation and inhibit thrombosis in thrombotic mice, and the effects of high concentrations of LP-KFY05 were most pronounced, which were similar to the effects of dipyridamole.

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Effect of soybean milk fermented with Lactobacillus plantarum HFY01 isolated from yak yogurt on weight loss and lipid reduction in mice with obesity induced by a high-fat diet

Abstract: Soybean milk fermented with Lactobacillus plantarum HFY01 (LP-HFY01) was used for weight and lipid reduction in mice with obesity induced by a high-fat diet.

Cited by 30 publications

References 58 publications

Protective Effects of Silymarin Against D-Gal/LPS-Induced Organ Damage and Inflammation in Mice

Protective Effects of Silymarin Against D-Gal/LPS-Induced Organ Damage and Inflammation in Mice

Antioxidant and Inflammatory Effects of Nelumbo nucifera Gaertn. Leaves

Lactobacillus plantarum HFY05 Attenuates Carrageenan-Induced Thrombosis in Mice by Regulating NF-κB Pathway-Associated Inflammatory Responses

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