Effect of soy saponin on the growth of human colon cancer cells

Tsai, Cheng‐Yu

doi:10.3748/wjg.v16.i27.3371

Cited by 55 publications

(28 citation statements)

References 19 publications

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“…Soyasaponin III followed a similar pattern with reductions of: 18.2, 32.7, and 40.0%. Depending on the soyasaponin, the percent reductions in PKC activity of this study are close to those of the previously mentioned studies (Tsai et al, 2010;Kim et al, 2004;Oh & Sung, 2001) which used soyasaponin mixtures. Soyasaponin I reduction was less than the reported values in these studies when used at the same concentrations, but soyasaponin III had a greater reduction at the 0.6 mg/ml concentration compared to mixtures of soyasaponins at that same concentration.…”

Section: Discussionsupporting

confidence: 87%

“…At the highest concentrations of soyasaponin and soyasapogenol B, complete deformation of Caco-2 cells was observed ( Figure 6). Similar results were seen by Tsai et al (2010). Changes in cell morphology are suggestive that soyasaponins may be binding to or inserting into the cell membrane.…”

Section: Discussionsupporting

confidence: 74%

“…Soyasaponin I had no effect on HT-29 colon cell growth (Sarkar & Li, 2003, Gurfinkel & Rao, 2003, but it decreased the migratory ability of B16F10 melanoma cells (Chang, Yu, Lin, Wang, & Tsai, 2006). Tsai et al (2010) reported that when WiDr human colon cancer cells treated with 0.15, 0.30, 0.60 or 1.2 mg/ml of crude soy saponin, the number of viable cells decreased in a dose-dependent manner and suppressed 12-Otetradecanol-phorbol-13-acetate-stimulated PKC activity (P < 0.05). Other biological activities such as Hepatoprotective activity on human-liver-derived cell line (HepG2 cells) also greatly differ with the type of saponins.…”

Section: Introductionmentioning

confidence: 98%

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Soyasaponin I, III, and Soyasapogenol B Inhibit Proliferation and Modulate PKC Expression in Caco-2 Human Colon Cancer Cells

Salyer¹,

Eswaranandam²,

Lee³

2013

JFR

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Group B saponins, the predominant form of saponins in heat-treated soy products, have been shown to possess hypocholesterolemic, antimutagenic, and anticarcinogenic properties. Previous studies have evaluated crude mixtures of soyasaponins, but studies evaluating a single purified soyasaponin as an anticarcinogenic agent are limited. The goal of this study is to examine the effects of purified soyasaponins I and III as well as their aglycone form, soyasapogenol B, as anticarcinogenic agents on the human colon adenocarcinoma cell line (Caco-2). Experiments were conducted to examine the effects of purified soyasaponins on cell proliferation, Protein Kinase C (PKC) activity, and cell morphology in Caco-2 cell cultures. Treatment of cells with soyasaponins I and III at concentrations of 0.3-0.9 mg/ml significantly reduced viable cell numbers after 48 and 72 hours of exposure by 8.6-65.3% (p < 0.05). Soyasapogenol B at a concentration of 0.15 mg/ml significantly reduced viable cell numbers after 24 hours by 62.4% (p < 0.05). Cell morphology changes demonstrated that as concentrations and lipophilicity of soyasaponins increased, cell membranes became rougher and more irregular. Treatment of cells for 72 hours significantly reduced the amount of PKC activity by 9-40% (p < 0.05). Results indicate that purified soyasaponins I, III and soyasapogenol B can suppress Caco-2 colon cancer cell proliferation. These findings suggest that purified group B soyasaponins and their final metabolite soyasapogenol B may be a colon-cancer suppressive component of soy that warrants further examination as a potential nutraceutical or functional food.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 98%

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Soyasaponin I, III, and Soyasapogenol B Inhibit Proliferation and Modulate PKC Expression in Caco-2 Human Colon Cancer Cells

Salyer¹,

Eswaranandam²,

Lee³

2013

JFR

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“…For instance, saponins appear to inhibit the infectivity of the AIDS virus (Nakashima et al 1989;Vlietinck et al 1998) and the herpes simplex virus type 1 (Hayashi et al 1997). Soyasaponins are reported to be hepatoprotective (Kinjo et al 2003) and have also shown a preventive effect on the growth of human colon cancer cells (Tsai et al 2010). The chemical structure significantly influences the saponins bioactivity; thus, individual soyasaponin I exhibits lipid peroxidation inhibitory effects in vitro, antiinflammatory properties in mice with TNBS-induced colitis (Lee et al 2010), and potentially reduces metastatic ability of cancer cells (Chang et al 2006).…”

Section: Saponinsmentioning

confidence: 99%

Soy and Soy-Based Foods: Role in Health and Nutrition

Villares¹,

García–Lafuente²,

Palacios³

et al. 2011

Soybean and Nutrition

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“…Epidemiological studies have shown that the association between the consumption of soy or soy products with reduced risk of colorectal cancer 9-11 . Soybeans contain a variety of phytochemicals, which demonstrated anticancer activity, including isoflavone 12 , protease inhibitor 13 , saponin 14, 15 , lectin 16 , and more recently lunasin, a novel cancer preventive seed peptide bioactive peptides involved in the preventive and therapy of cancer 6, [16][17][18] . This study explores the use of Indonesian native plants, which was Grobogan soybean variety.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Activity of Indonesian Soybean in comparison with Soybean Meal on Goblet Cell Count and Micro Vessel Density in Colitis-Associated Mouse Colon Carcinogenesis

Putri¹,

Kusmardi²,

Elya³

2017

IJPTR

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: Colon cancer is a major public health problem throughout the worldwide. Recently, many studies have focused on finding chemoprevention based on the natural compound. The present study was aimed to investigate the inhibitory activity of Indonesian soybean in comparison with soybean meal extract in colitis-associated mouse colon carcinogenesis. Methods: In this study, Swiss Webster mice were induced by azoxymethane 10 mg/kg body weight followed by administration of 2% dextran sodium sulfate during a week. Soybean, soybean meal extract at each dose of 75, 150, and 200 mg daily and aspirin 0,39 mg/kg body weight daily (equivalent to 150 mg for a 70 kg human) administered orally. Histopathological examination of the colon tissue (hematoxylin-eosin staining) was done by counting the number of goblet cells and micro vessel density in ten randomly selected fields visual. The results: Soybean and soybean meal significantly reduced the count of goblet cell (P<0,05) in colitis-associated colon carcinogenesis mice. Soybean extract is also significantly reduced the number of micro vessel density (P<0,001) in colitis-associated colon carcinogenesis mice. Otherwise, there were no significant effects of the soybean meal extract on the number of micro vessel density in colitis-associated colon carcinogenesis mice. Conclusion: Our results indicate that Indonesian soybean and soybean meal extract may have inhibitory activity in colitis-associated colon carcinogenesis through inhibiting reduction in the number of goblet cell and micro vessel density. Indonesian soybean may have Inhibitory activity better than soybean meal.

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Effect of soy saponin on the growth of human colon cancer cells

Abstract: Soy saponin may be effective in preventing colon cancer by affecting cell morphology, cell proliferation enzymes, and cell growth.

Cited by 55 publications

References 19 publications

Soyasaponin I, III, and Soyasapogenol B Inhibit Proliferation and Modulate PKC Expression in Caco-2 Human Colon Cancer Cells

Soyasaponin I, III, and Soyasapogenol B Inhibit Proliferation and Modulate PKC Expression in Caco-2 Human Colon Cancer Cells

Soy and Soy-Based Foods: Role in Health and Nutrition

Inhibitory Activity of Indonesian Soybean in comparison with Soybean Meal on Goblet Cell Count and Micro Vessel Density in Colitis-Associated Mouse Colon Carcinogenesis

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