Effect of sonication pretreatment on physico-chemical, surface and thermal properties of date palm pollen protein concentrate

Effect of sonication pretreatment on physicochemical, surface, thermal, and functional properties of fibro‐proteic extracts from male date palm flowers

“…The obtained values indicated high thermal stability of both extracts. A similar tendency was noticed with native and sonicated date palm pollen concentrates (Sebii et al., 2019). The mentioned decrease of ΔH means that less energy was needed to engender the protein unfolding of FPEUS.…”

“…In the studies of Sebii et al. (2019), an inverse relationship between protein content and surface activity of native and sonicated date palm pollen protein concentrates was found, which was explained by the presence of nonprotein surfactant in the extracts: the saponin. Blecker et al.…”

“…The PS was important for both extracts and increased from 91.1% for FPE to total protein solubility for FPEUS. Previous works have reported that sonication affects the protein solubility and that its decrease reflects its denaturation (Ghribi Mokni et al., 2015; Sebii et al., 2019). However, in the current study, the ultrasound pretreatment has enhanced the protein solubility, which could be explained by less formation of aggregates due to the exposure of negative residues and more breakages in the intramolecular bonds (Nazari et al., 2018).…”

“…An aqueous 10% of MDPF solution was prepared at pH 12, then, the ultrasonic treatment was applied as reported by Sebii et al. (2019) using a probe system (Vibracell 75,043, Bioblock Scientific, USA) (Figure 1) at the frequency of 20 kHz and the amplitude of 60% for 2 s, followed by a 1‐s pulse‐off delay with a total sonication time of 30 min. The sample temperature was controlled at 25°C by cooling in an ice bath to avoid the effect of temperature during the process.…”

“…Recent works have reported the effects of sonication on the physicochemical, structural, and functional properties of proteins such those performed on canola protein isolate (Flores‐Jiménez et al., 2019), date palm pollen concentrate (Sebii et al., 2019), and millet protein concentrate (Nazari et al., 2018). However, it was found that the benefits of using this technology depend on the native characteristics of the protein and its source, the conditions of the ultrasound treatment, the temperature, the pH, the time, the ionic strength, and all of the variables that affect the physicochemical properties of proteins.…”