Effect of sonicated extracts ofEnterococcus faecalison the production of matrix metalloproteinase-8 by human polymorphonuclear neutrophils

Abstract

This in vitro study monitored MMP-8 production on PMN by stimulated with the following three groups; Sonicated extracts of E. faecalis (SEF), SEF treated with Ca(OH)2 (12.5㎎/㎖) for 7 days, and lipopolysaccharides (LPS) of E. coli. The level of MMP-8 in each group was immediately measured by ELISA. The data were analyzed with Kruskal-Wallis test and Mann-Whitney U test.In the SEF group, the level of production of MMP-8 was higher than the negative control group in low concentration (0.05㎍/㎖) of SEF (p < 0.05), but it decreased with an increase in the concentration of SEF (p < 0.05). In the case of SEF treated with Ca(OH)2, all of the MMP levels were higher than negative control group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05). All of the levels in E. coli LPS were increased with increasing concentrations (p < 0.05).According to this study we could summarize as follows: 1. MMP-8 was expressed at low level in untreated PMN group and the levels of MMP-8 were upregulated in PMN stimulated by E. coli LPS groups. 2. In the SEF groups, the level of production of MMP-8 decreased with an increase in the concentration of SEF (p < 0.05). So E. faecalis may have suppressive effect on the production of MMP-8 by PMN. 3. In the case of SEF treated with Ca(OH)2, all of the MMP levels at different SEF concentrations were higher than untreated PMN group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05). [J Kor Acad Cons Dent 30(2):138-144, 2005]

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

“…Simpulan: Ekstrak daun jeruk purut memiliki aktivitas antibakteri terhadap bakteri Enteroccus faecalis dan konsentrasi 16% merupakan konsentrasi optimal dibandingkan dengan konsentrasi 4% dan 8%. Pada kandungan tersebut mekanisme antibakteri yaitu mengganggu komponen penyusun peptidoglikan pada dinding sel bakteri, 10 memecah komponen lipid dalam membran sel bakteri, 11 mengubah permeabilitas dinding sel bakteri, mengganggu stabilitas membran sel bakteri, 12 mendenaturasi protein sel bakteri sehingga aktivitas sel terganggu, menyebabkan keluarnya isi sel bakteri, dan akhirnya bakteri akan lisis atau mati. 13,14 Kandungan minyak atsiri pada daun jeruk purut mampu menghambat pembentukan biofilm bakteri S. mutans.…”