Effect of some plant flavonoids on in vitro behaviour of an arbuscular mycorrhizal fungus

Morandi, Dominique; Branzanti, B.; Gianinazzi‐Pearson, Vivienne

doi:10.1051/agro:19921012

Cited by 36 publications

(28 citation statements)

References 16 publications

(20 reference statements)

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“…This effect may due to the enhancement of phenol production in host tissues. This results are in agreement with Morandi et al [20], Metraux and Raskin [21] who emphasized that phenolic phytoalexin may involved in the resistance of plant to pathogenic microbial infection.…”

Section: Discussionsupporting

confidence: 93%

New Strategy for Managing Damping-off and Root Rot Disease of Cucumber Caused by Rhizoctonia solani by Seed Soaking in Formula of Antioxidant with Micronutrients

El-Metwally¹

2013

J Plant Pathol Microb

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Section: Discussionsupporting

confidence: 93%

New Strategy for Managing Damping-off and Root Rot Disease of Cucumber Caused by Rhizoctonia solani by Seed Soaking in Formula of Antioxidant with Micronutrients

El-Metwally¹

2013

J Plant Pathol Microb

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“…Coumestrol has been shown to be stimulatory to hyphal growth and root colonization (Morandi et al, 1992;Xie et al, 1995). AMF may also have adapted to recognizing this signal compound which was accumulated in the second greatest amount.…”

Section: Discussionmentioning

confidence: 99%

Specific flavonoids as interconnecting signals in the tripartite symbiosis formed by arbuscular mycorrhizal fungi, Bradyrhizobium japonicum (Kirchner) Jordan and soybean (Glycine max (L.) Merr.)

Antunes

Rajcan

Goss

2006

Soil Biology and Biochemistry

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Many legume plants benefit from the tripartite symbiosis of arbuscular mycorrhizal fungi (AMF) and rhizobia. Beneficial effects for the plant have been assumed to rely on increased P supply through the mycorrhizas. Recently, we demonstrated that P does not regulate the establishment of the tripartite symbiosis. Flavonoids appear to play a role as early signals for both rhizobia and AMF. Four soybean lines known to express different concentrations of the isoflavones genistein, daidzein and glycitein in the seed were used to test three hypotheses: (i) The establishment of the tripartite symbiosis is not dependent of a nutrient mediated effect; (ii) There is a positive correlation between seed isoflavone concentrations of different soybean lines and the progress of the tripartite symbiosis; (iii) Specific flavonoids control the establishment of the tripartite symbiosis in that a change in flavonoid root accumulation resulting from the development of one microbial partner can stimulate colonization of soybean roots by the other. Disturbed versus undisturbed soil treatments were produced to vary the potential for indigenous AMF colonization of soybean. In contrast, the potential for Bradyrhizobium was kept identical in both soil disturbance treatments. The uptake of P and Zn and the concentration of flavonoids in mycorrhizal soybean roots at 10 d after emergence were analysed either separately of Bradyrhizobium or in context of the tripartite symbiosis. Zinc nutrition did not differ between AMF treatments which supports the first hypothesis. The concentration of daidzein was at least four times greater in the root than in the seed reaching 3958G249 mg g K1 dry across soybean lines. Coumestrol, which was absent in the seed, was synthesized to reach 2154G64 mg g K1 dry. Conversely, the concentration of genistein was approximately three times smaller in the root that in the seed (301G15 mg g K1 dry), while glycitein and formononetin were never detected. The establishment of the tripartite symbiosis was identical across soybean lines which does not support the second hypothesis. Concentrations of flavonoids were significantly greater in roots under disturbed soil, for which both symbioses were not as developed as in plants from undisturbed soil. This clearly supports the third hypothesis. This research provides the first data linking the function of different flavonoids to the establishment of the tripartite symbiosis, and suggests that these compounds are produced and released into the rhizosphere as a function of the colonization process. q

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“…Coumestrol is capable of stimulating hyphal growth and root colonization (Morandi et al, 1992;Xie et al, 1995) as well as inducing nod genes in Bradyrhizobium (e.g. Kosslak et al, 1987).…”

Section: Establishment Of the Tripartite Symbiosismentioning

confidence: 99%

Accumulation of specific flavonoids in soybean (Glycine max (L.) Merr.) as a function of the early tripartite symbiosis with arbuscular mycorrhizal fungi and Bradyrhizobium japonicum (Kirchner) Jordan

Antunes

Varennes²,

Rajcan

et al. 2006

Soil Biology and Biochemistry

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Accumulation of specific flavonoids in soybean (Glycine max (L.) Merr.) as a function of the early tripartite symbiosis with arbuscular mycorrhizal fungi and Bradyrhizobium japonicum (Kirchner) Jordan AbstractThis study is the first report assessing the effect of soil inoculation on the signalling interaction of Bradyrhizobium japonicum, arbuscular mycorrhizal fungi (AMF) and soybean plants throughout the early stages of colonisation that lead to the tripartite symbiosis. In a study using soil disturbance to produce contrasting indigenous AMF treatments, the flavonoids daidzein, genistein and coumestrol were identified as possible signals for regulating the establishment of the tripartite symbiosis. However, it was unclear whether soil disturbance induced changes in flavonoid root accumulation other than through changing the potential for AMF colonization. In this study, soil treatments comprising all possible combinations of AMF and B. japonicum were established to test whether (1) modifications in root flavonoid accumulation depend on the potential for AMF colonization, and (2) synthesis and accumulation of flavonoids in the roots change over time as a function of the early plant-microbial interactions that lead to the tripartite symbiosis. The study was comprised of two phases. First, maize was grown over 3-week periods to promote the development of the AM fungus Glomus clarum. Second, the interaction between soybean, G. clarum and B. japonicum was evaluated at 6, 10, 14 and 40 days after plant emergence. Root colonization by G. clarum had a positive effect on nodulation 14 days after emergence, producing, 30% more nodules which were 40% heavier than those on roots solely inoculated with B. japonicum. The tripartite symbiosis resulted in 23% more N 2 being fixed than did the simpler symbiosis between soybean and B. japonicum. The presence of both symbionts changed accumulation of flavonoids in roots. Daidzein and coumestrol increased with plant growth. However, development of the tripartite symbiosis caused a decrease in coumestrol; accumulation of daidzein, the most abundant flavonoid, was reduced in the presence of AMF. q

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Effect of some plant flavonoids on in vitro behaviour of an arbuscular mycorrhizal fungus

Abstract: Summary — The effect of 2 isoflavonoids (the soybean phytoalexin, glyceollin I, and the coumestan, coumestrol) and 1 flavonoid, quercetin, were tested on in vitro spore germination of Gigaspora margarita. Glyceollin I and coumestrol were tested at 0, 0.05, 0.5, 5

Cited by 36 publications

References 16 publications

New Strategy for Managing Damping-off and Root Rot Disease of Cucumber Caused by Rhizoctonia solani by Seed Soaking in Formula of Antioxidant with Micronutrients

New Strategy for Managing Damping-off and Root Rot Disease of Cucumber Caused by Rhizoctonia solani by Seed Soaking in Formula of Antioxidant with Micronutrients

Specific flavonoids as interconnecting signals in the tripartite symbiosis formed by arbuscular mycorrhizal fungi, Bradyrhizobium japonicum (Kirchner) Jordan and soybean (Glycine max (L.) Merr.)

Accumulation of specific flavonoids in soybean (Glycine max (L.) Merr.) as a function of the early tripartite symbiosis with arbuscular mycorrhizal fungi and Bradyrhizobium japonicum (Kirchner) Jordan

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