While nano-hydroxyapatite (nano-HAP) has been well known for series of amazing properties in chemical or physical, the controversy on the risks of its applications has also been existed. The worries of nano-HAP applications in preclinic and clinic indicate the blank researches of nano-HAP pharmacodynamics. It is important and necessary to trace and clarify the localizations of HAP nanoparticles in vivo. In the present paper, 18F is used as radiotracer for Positron Emission Tomography (PET) imaging of HAP nanoparticles. Through the transverse plane slices and three-dimensional reconstruction pictures, it is very clear to observe the localization of nano-HAP in vivo at real time. Most nano-HAP particles were noted in organs lump, liver, spleen, stomach and existed for period of time. Therefore, PET can be a new powerful technique for tracing nano-biomaterial and their pharmacodynamics researches.