Effect of Some Environmental Parameters on Hydrogen Production Using C. acetobutylicum

Abstract

The aim of this study was to investigate the influence of some environmental factors on bacterial metabolism. Fermentative hydrogen production by C. acetobutylicum, using glucose as the substrate. The effect of initial pH (4-8), inoculum size (1-20% (v/v)) and glucose concentration (1-30 g L(-1)) on hydrogen production were studied. The optimum cultivation temperature for hydrogen production was at 30 degrees C. The results show that substrate concentration and inoculum size resulted in hydrogen yield (Y(P/S)) of 391 mL g(-1) glucose utilized with maximum hydrogen productivity of 77.5 mL/L/h. Higher substrate concentration or inoculum size adversely affects hydrogen production, which decreases hydrogen yield by 15% to 334 mL g(-1) glucose utilized when 30% (v/v) inoculum size was used. The use of 30 g L(-1) substrate concentration resulted in a 75% decrease to 97 mL g(-1) glucose supplied. Concluded that proper Xo/So enhanced the hydrogen production.

Selection and identification of microorganisms present in the treatment of wastewater and activated sludge to produce biohydrogen from glycerol

“…Sodium is required by microorganisms in different quantities, since it regulates cell osmosis. At high concentrations, this element may inhibit cell growth [1,19]. Finally, iron influences cell growth and takes participates in the synthesis of several proteins, in electron transport, and in the constitution of cytochromes, in addition to hydrogenases functioning [1,19].…”

“…It is an essential element for the synthesis of proteins, amino acids and other cellular compounds [1,19].For the stability of the ribosome and synthesis of many enzymes, magnesium is essential [1,19]. Calcium helps in the stabilization of cell walls and in the thermostability of endospores [1,19]. Sodium is required by microorganisms in different quantities, since it regulates cell osmosis.…”

“…After carbon, nitrogen is the second most important macronutrient for microbial growth [1,19]. It is an essential element for the synthesis of proteins, amino acids and other cellular compounds [1,19].For the stability of the ribosome and synthesis of many enzymes, magnesium is essential [1,19].…”

“…After that, the gas was bubbled into the inverted flask containing a solution of NaOH at 5%, causing the CO 2 present in the biogas to react with the sodium hydroxide, according to Equation (1).…”

Investigating the effects of iron and nickel nanoparticles on dark hydrogen fermentation from starch using central composite design

“…However, at higher concentrations, iron and nickel may inhibit the process [11]. Alshiyab et al (2008) investigated the impact of Fe 2þ ions concentration (0e1000 mg/L) on the batch hydrogen fermentation from glucose using Clostridium acetobutylicum, and reported that the hydrogen yield was enhanced from 391 to 408 mL/g-VS at Fe 2þ ions concentration of 25 mg/L, while the higher concentrations of Fe 2þ ions negatively affected the yield [13]. Wang and Wan (2008) investigated the impact of Ni 2þ ions on the batch hydrogen fermentation using mixed culture and reported that Ni 2þ ions concentrations below 0.1 mg/L could increase the hydrogen yield.…”