Effect of some antigens on leucocyte cultures

Inhibition of Tuberculin-Induced Mitogenesis in Cultures of Lymphocytes from Tuberculous Donors

“…Colchicine (2. (1,3,14). Care was taken to distinguish transformed lymphocytes from medium-sized and large phagocytic cells.…”

Prednisolone Inhibition of DNA Synthesis by Human Lymphocytes Induced in vitro by Phytohaemagglutinin

“…Qualitatively simi lar changes occurring later and to a lesser degree are induced by specific antigens (4,14). As corticosteroids are known to be lymphopenic in vivo and in vitro (7) their effect on PHA mitogenesis in vitro has previously been studied (3,6,9,12).…”

Mitogenic Activity of Bacterial Fractions in Lymphocyte Cultures

“…This phenomenon, as well as responsiveness to very small amounts of endotoxin had previously been described by O ppe n h e im and P e r r y [5]. An increase in the rate of transformation in cultures incubated longer than four days may have contributed to high values in some instances [3,5,6].…”

“…Criteria for estimating the proportion of transformed cells were not described in detail. C o w lin g and Q uaglino [3] examined the effect of an optimal concentration (1-250) of TAB on cultured lymphocytes from previously-immunized subjects. The average rate of transforma tion w'as rather low at three days of incubation, but somewhat higher values were obtained at 5 and 7 days.…”