Effect of some amino acids and peptides on silicic acid polymerization

Coradin, Thibaud; Livage, Jacques

doi:10.1016/s0927-7765(01)00143-6

Cited by 205 publications

(196 citation statements)

References 16 publications

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“…Bis-tris propane (1,(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) Bis(tris(hydroxymethyl)methylamino)propane,99%) was obtained from Acros Chemicals. Chemicals were used as received.…”

Section: Methodsmentioning

confidence: 99%

“…a protein) acts as a template or guides mineral formation, often 30 controlling the mineral's growth, resulting in materials with superior properties. [1][2][3] The bottom-up biomimetic approach to the formation of materials is a promising method, that achieves high level of control under mild synthetic conditions and has been successfully applied to silica and other minerals, 4-7 using peptides 35 and macromolecules to promote/ template the formation of specific nanostructures. Germanium dioxide, or germania (GeO 2 ), is a chemical analogue of silica with two main polymorphs: hexagonal (trigonal) quartz-like structure; and tetragonal (cubic) rutile-like 40 structure.…”

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confidence: 99%

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Preparation of hexagonal GeO₂ particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras

2014

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We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO 2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO 2 was prepared from germanium tetraethoxide (TEOG) in the presence of different silk-based proteins: Bombyx mori silk (native silk) and two chimeric proteins prepared by linking a germania binding peptide (Ge28: HATGTHGLSLSH) with Bombyx mori silk via chemical coupling at different peptide loadings (silk-Ge28 10% and silk-Ge28 50%). The mineralisation 10 activity of the silk-based proteins was compared with that of peptide Ge28 as a control system. GeO 2 mineralisation was investigated in water and in citric acid/bis-tris propane buffer at pH 6. Morphology, particle size, crystallinity, water and organic content of the materials obtained were analysed to study the effect of added biomolecules and mineralisation environment on material properties. In the presence of silk additives well-defined cube-shape hybrid materials composed of hexagonal germania and up to ca. 5 wt% organic content were obtained. The cubic particles 15 ranged from 0.4 to 1.4 m in size and were composed of crystalline domains in the range 35-106 nm depending on the additive used and synthesis conditions. The organic material incorporated in the mineral did not appear to affect the unit cell dimensions. The silk and chimeric proteins in water promote material formation and crystal growth, possibly via an effective ion-channelling mechanism. The germania binding peptide alone did not have any significant effect on reaction rate, yield or the material's properties compared to the blank. Interestingly, the peptide 20 content in the silk chimeras tested did not affect mineralisation. The presence of buffer inhibited mineral condensation rate and yield. The use of silk-based biomolecules allows control of crystallite/particle size of hybrid materials opening up opportunities for bio-inspired approaches to be applied for the synthesis of functional germania based devices and materials.

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“…Bis-tris propane (1,(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) Bis(tris(hydroxymethyl)methylamino)propane,99%) was obtained from Acros Chemicals. Chemicals were used as received.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Preparation of hexagonal GeO₂ particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras

2014

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“…FTIR and Confocal Micro Raman spectroscopy studies were carried out to confirm the presence of insulin, silica coating, and biologically active state of insulin in the formulations. Silica coated insulin loaded PC nanosphere formulation having infrared absorption (Figure 4 [37]. Similarly Si-O tetrahedral vibration at 495 cm −1 as observed in MicroRaman also indicates presence of silica coating.…”

Section: Resultsmentioning

confidence: 66%

Silica-Coated Liposomes for Insulin Delivery

Dwivedi

Arunagirinathan

Sharma

et al. 2010

Journal of Nanomaterials

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Liposomes coated with silica were explored as protein delivery vehicles for their enhanced stability and improved encapsulation efficiency. Insulin was encapsulated within the fluidic phosphatidylcholine lipid vesicles by thin film hydration at pH 2.5, and layer of silica was formed above lipid bilayer by acid catalysis. The presence of silica coating and encapsulated insulin was identified using confocal and electron microscopy. The native state of insulin present in the formulation was evident from Confocal MicroRaman spectroscopy. Silica coat enhances the stability of insulin-loaded delivery vehicles. In vivo study shows that these silica coated formulations were biologically active in reducing glucose levels.

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“…However, the dissolved Si may indicate a source of the observed reduction; as the Si-O network is broken there is a quantity of dissolved silica in solution most commonly ortho-silicic acid at a pH between 4 and 9 [8]. At a higher pH (greater than 8) it has been shown that polymerization of the ortho-silicic acid occurs producing a variety of negatively charged silicic acid species carrying a charge from −1 to −4 at a pH of 12 [58][59][60]. The existence of these negatively charged species may account for the neutralization of the ABTS radical cation through strong electrostatic interactions or electron donation thereby reducing the ionic charge on the silicic acid polymers.…”

Section: Discussionmentioning

confidence: 99%

Investigating the Effect of Glass Ion Release on the Cytocompatibility, Antibacterial Eflcacy and Antioxidant Activity of Y2O3 / CeO2 doped SiO2-SrO-Na2O glasses

et al. 2018

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Abstract:The effect on ion release and cytocompatibility of Yttrium (Y) and Cerium (Ce) are investigated when substituted for Sodium (Na) in a 0.52SiO 2 -0.24SrO-0.24-Na 2 O-MO glass series (where MO = Y 2 O 3 or CeO 2 ). Glass leaching was evaluated through pH measurements and Inductive Coupled Plasma-Optical Emission Spectrometry (ICP-OES) analysis where the extract pH increased during incubation (11.2 -12.5). Ion release of Silicon (Si), Na and Strontium (Sr) from the Con glass was at higher than that of glasses containing Y or Ce, and reached a limit after 1 day. Ion release from Y and Ce containing glasses reached a maximum of 1800 µg/mL, 1800 µg/mL, and 10 µg/mL for Si, Na, and Sr, respectively. Release of Y and Ce was below the ICP-OES detection limit <0.1 µg/mL. Cell viability of both L929 fibroblasts and MC3T3 osteoblasts decreased for Con, LY, and LCe extracts; HY extracts did not significantly decrease cell viability while YCe and HCe saw concentrationdependent viability decrease (20%, 33% extract concentrations). Bacterial studies saw Con and LCe eliminating >75% of bacteria at a 9% extract concentration. Antioxidant capacity (mechanism for neuroprotection) was evaluated using the ABTS assay. All glasses had inherent radical oxygen species (ROS) scavenging capability with Con reaching 9.5 mMTE.

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Effect of some amino acids and peptides on silicic acid polymerization

Cited by 205 publications

References 16 publications

Preparation of hexagonal GeO₂ particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras

Preparation of hexagonal GeO₂ particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras

Silica-Coated Liposomes for Insulin Delivery

Investigating the Effect of Glass Ion Release on the Cytocompatibility, Antibacterial Eflcacy and Antioxidant Activity of Y2O3 / CeO2 doped SiO2-SrO-Na2O glasses

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Effect of some amino acids and peptides on silicic acid polymerization

Cited by 205 publications

References 16 publications

Preparation of hexagonal GeO2 particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras

Preparation of hexagonal GeO2 particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras

Silica-Coated Liposomes for Insulin Delivery

Investigating the Effect of Glass Ion Release on the Cytocompatibility, Antibacterial Eflcacy and Antioxidant Activity of Y2O3 / CeO2 doped SiO2-SrO-Na2O glasses

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Product

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Preparation of hexagonal GeO₂ particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras

Preparation of hexagonal GeO₂ particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras