Effect of somatostatin on d-galactose transport across the small intestine of rats

Sorribas, Vı́ctor; Arruebo, Pilar; murillo, daniel; Alcalde, Ana Isabel

doi:10.1016/0300-9629(87)90277-5

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…Not only VIP, but several intestinal hormones have been shown to interfere with the intestinal transport of nutrients ( SORRIBAS et al, 1987). Furthermore, theophylline, and exogenous intestinal secretagogue which increases cyclic AMP, has been described as an inhibitor of intestinal sugar transport ( RANDLES and KIMMICH, 1978;ILUNDAIN et al, 1985) and this inhibition seems to be mediated by the increase in both cyclic AMP and intracellular free calcium.…”

Section: Introductionmentioning

confidence: 99%

Effect of VIP on Sugar Transport in Rabbit Small Intestine in vitro

Arruebo

Sorribas

Rodríguez-Yoldi

et al. 1990

Journal of Veterinary Medicine Series A

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The vasoactive intestinal peptide (VIP) has shown to be widely distributed in the gastrointestinal mucosa, submucosa and nerves, and the existence of VIP receptors on the basolateral membrane of enterocytes has been recently reported for many species. The interaction of VIP with its receptors seemed to increase cyclic AMP level, and this nucleotide has been shown to be responsible for the intestinal secretion produced by VIP. The present study confirms that VIP inhibits the intestinal absorption of D-galactose. This effect seems to be due to the inhibition of the Na+-independent basolateral intestinal sugar transport system. RMI 12330A, described as adenylate cyclase inhibitors, blocked the VIP action. These findings suggest that cyclic AMP might be responsible for the inhibition of Na+-independent transport of D-galactose across the basolateral membrane. Moreover, results obtained to determine the possible role of calcium in the action of VIP suggest that Ca*+ play a part, directly or indirectly, in the inhibition of the D-galactose transport across the basolateral membrane produced by VIP.

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Section: Introductionmentioning

confidence: 99%

Effect of VIP on Sugar Transport in Rabbit Small Intestine in vitro

Arruebo

Sorribas

Rodríguez-Yoldi

et al. 1990

Journal of Veterinary Medicine Series A

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“…However, as some secretagogues like theophylline (Randles et Kimmich, 1978;Ilundain et al, 1985) and gastrointestinal hormones such as somatostatin (Sorribas et aL, 1987) (Sheffe, 1953 Table 111). (Gershon et al, 1985).…”

Section: Serotonin (5-hydroxytryptamine) (5 Ht)mentioning

confidence: 99%

Effect of serotonin on D-galactose transport across the rabbit jejunum

Arruebo¹,

Mesonero²,

Murillo³

et al. 1989

Reprod. Nutr. Dévelop.

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Summary ― The patterns of storage and release of serotonin found in the enterochromaffin cells of the intestinal mucosa suggest that this hormone may be an important modulator of intestinal functions. Serotonin has been shown to produce secretion of water and electrolytes in rabbit ileum, but the hormone does not appear to interact significantly with other transport processes. The aim of the present study was to determine the effect of serotonin on D-galactose absorption in rabbit jejunum. The results obtained show that serotonin (10--8 and 10--6 M) partially reduced (by 20 and 40 % respectively) D-galactose uptake across the mucosal border. This effect was concentrationdependent, and it seemed to be caused by the inhibition of Na+-dependent sugar transport. Methysergide, an antagonist of serotonin which binds with receptor 2 of serotonin, blocked the effect of serotonin. These findings suggest that serotonin may act as a regulator of sugar intestinal absorption, and that this serotonin regulation could be mediated by a direct or indirect action of the complex serotonin―receptor, which may inhibit the Na+-dependent transport system of sugars located in the brush-border membrane.serotonin -D-galactose -intestinal transport -rabbit Résumé ― Effet de la sérotonine sur le transport du D-galactose dans le jéjunum du lapin. La sérotonine est une hormone gastro-intestinale qui se trouve principalement dans les cellules entérochromaffines de la muqueuse intestinale. L'étude du stockage comme de la libération de la sérotonine dans le tractus gastro-intestinal, a suggéré que la sérotonine peut agir comme modulateur des fonctions intestinales. La sérotonine produit la sécrétion d'eau ainsi que des électrolytes dans I iléum du lapin, mais cette hormone n'a montré aucune interaction significative avec les autres processus de transport intestinal. Le but de ce travail a été de déterminer l'effet de la sérotonine sur l'absorption du D-galactose dans le jéjunum du lapin. Les résultats obtenus montrent que la sérotonine (10-e et 10-6 M) a diminué partiellement (20 et 40 % respectivement) l'entrée du D-galactose dans la muqueuse. Cet effet dépend du degré de concentration de la sérotonine et il nous semble qu'il pourrait être une inhibition du transport des sucres dépendant du Na+. La métisergide, un antagoniste de la sérotonine qui se lie au récepteur 2 de la sérotonine, a inhibé l'effet de la sérotonine. Ces résultats suggèrent que la sérotonine pourrait jouer une fonction régulatrice sur l'absorption intestinale des sucres et que cette régulation pourrait aussi être médiée par une action directe ou indirecte du complexe récepteur-sérotonine inhibé par le système de transport des sucres dépendant du Na+ situé dans la membrane de la bordure en brosse.sérotonine -D-galactose -transport intestinal -lapin

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Effects of somatostatin on luminal transit and absorption of nutrients in the proximal gut of minipigs

Eisenbraun

Ehrlein

1996

Digest Dis Sci

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A discrepancy exists on the effects of somatostatin on the absorption of nutrients: in humans, absorption was found to be reduced, whereas in rats no effects were observed. However, intestinal absorption might be influenced by the transit rate of contents. This was not considered in previous studies. Therefore, we investigated simultaneously the effects of somatostatin on the absorption of nutrients and on luminal transit. In five minipigs (44-62 kg), a 150-cm segment of the proximal jejunum was temporarily isolated by two cannulas and perfused with an oligomer diet (60% carbohydrate, 18% protein, and 22% fat). The perfusion rate was 2 kcal/min. Flow rate and mean transit time were determined by markers (Cr-EDTA and Cu-EDTA). Somatostatin was infused intravenously at rates of 0.5, 1.25, 2.5, and 5 micrograms/kg/hr. In control experiments saline was administered intravenously. Somatostatin dose-dependently diminished flow rate of luminal contents and increased the transit time. At the largest dose of somatostatin (5 micrograms/kg/hr) flow rate was reduced by 50% compared with control infusion of saline (1.0 +/- 0.4 vs 2.0 +/- 0.05 ml/min, P < 0.05), and transit time was increased 3.6-fold (39.8 +/- 4.7 vs 11.2 +/- 4.9 min; P < 0.05). Somatostatin also dose-dependently enhanced the absorption of nutrients and energy. However, the increase in absorption was small compared with the effects on flow rate and transit time. At the largest dose (5 micrograms/kg/hr) absorption of energy, carbohydrate, protein, and fat was enhanced only by 9.7%, 7.0%, 5.2%, and 15.3%, respectively (49.9 vs 40.2%, 50.9 vs 43.9%, 67.3 vs 62.1%, and 30.1 vs 14.8% during saline infusion; P < 0.05). Results indicate that the major effects of somatostatin consist in a marked reduction of flow rate and a delay of luminal transit. The small increase in absorption was caused by the delay in transit and the prolonged contact of the nutrients with the mucosa. Therefore, in absorption studies, effects on transit need to be considered.

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Effect of somatostatin on d-galactose transport across the small intestine of rats

Cited by 6 publications

References 18 publications

Effect of VIP on Sugar Transport in Rabbit Small Intestine in vitro

Effect of VIP on Sugar Transport in Rabbit Small Intestine in vitro

Effect of serotonin on D-galactose transport across the rabbit jejunum

Effects of somatostatin on luminal transit and absorption of nutrients in the proximal gut of minipigs

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