Effect of solvents and oil content on direct transesterification of wet oil-bearing microalgal biomass of Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized lipase as the biocatalyst

Tran, Dang Thuan; Chen, Ching Lung; Chang, Jo Shu

doi:10.1016/j.biortech.2012.09.101

Cited by 117 publications

(37 citation statements)

References 34 publications

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“…To achieve this, water must be made available to the largest-possible portion of the oil, and a good measure of agitation is known to create a faster reaction and higher conversion yields [32,33].…”

Section: Agitation Effect and Improvement Of The Conversion Efficiencmentioning

confidence: 99%

Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase

Amoah

Hama³

et al. 2016

Biochemical Engineering Journal

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Graphical abstract HighlightsBioconversion of high phospholipid-containing oilThe catalyst used was immobilized whole-cell A. oryzae-expressing FHL Effect of reverse micelle formation on bioconversionThe reduction of reverse micelle formation was crucial for bioconversion Conversion was improved by approximately 3-fold to more than 90% AbstractThe presence of phospholipids in oil has been a major hurdle in the production of biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts. A density of phospholipids within the range of 10 to 30% could reduce both the rate of production and the final yield of biodiesel. Phospholipids in the oil leads to the formation of water-in-oil phospholipid-based reverse micelles. The water that activates the enzymatic process is observed to be trapped inside these reverse micelles. This has resulted in the inactivation of the reaction systems and has subsequently led to the deactivation of the immobilized lipase by the extended residence time of the added methanol. A reaction system involving gentle agitation and higher amount of water was found to reduce the reverse micelles formation. This simple technique improved the conversion efficiency by approximately 3-folds, producing a final biodiesel of more than 90 %, using immobilized A. oryzae whole cells expressing Fusarium heterosporum lipase. This demonstrates that, the above technique could be successfully applied to the enzymatic biodiesel conversion of oils containing high amounts of phospholipids such as that from microalgae.

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Section: Agitation Effect and Improvement Of The Conversion Efficiencmentioning

confidence: 99%

Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase

Amoah

Hama³

et al. 2016

Biochemical Engineering Journal

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show abstract

“…In recent years, the in situ transesterification process was developed to directly convert wet oil-bearing microalgae biomass into biodiesel. Dang-Thuan et al (2013) reported that Chlorella vulgaris ESP-31 biomass (water content of 86-91%, oil content of 14-63%) was pretreated by sonication to disrupt the cell walls and then directly mixed with methanol and solvent to carry out the enzymatic transesterification and achieve over 90% biodiesel conversion.…”

Section: In Situ Transesterificationmentioning

confidence: 99%

Current Status and Outlook in the Application of Microalgae in Biodiesel Production and Environmental Protection

et al. 2014

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“…Therefore, recent studies have focused on using ''wet microalgae'' as the raw materials to produce biodiesel (Olmstead et al, 2013;Tran et al, 2013). In addition, some of the downstream technologies still suffer the drawbacks of high energy consumption and low treatment capacity (as seen, for example, with centrifugation and supercritical fluid extraction), limiting the commercialization of these methods.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel production from wet microalgae feedstock using sequential wet extraction/transesterification and direct transesterification processes

Chen

Huang

et al. 2015

Bioresource Technology

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107

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Effect of solvents and oil content on direct transesterification of wet oil-bearing microalgal biomass of Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized lipase as the biocatalyst

Cited by 117 publications

References 34 publications

Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase

Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase

Current Status and Outlook in the Application of Microalgae in Biodiesel Production and Environmental Protection

Biodiesel production from wet microalgae feedstock using sequential wet extraction/transesterification and direct transesterification processes

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